Orally administered peptides to ameliorate atherosclerosis

ABSTRACT

This invention provides novel peptides that ameliorate one or more symptoms of atherosclerosis. The peptides are highly stable and readily administered via an oral route.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation-in-part of U.S. Ser. No.09/896,841, filed on Jun. 29, 2001, which is a continuation-in-part ofU.S. Ser. No. 09/645,454, filed on Aug. 24, 2000, both of which areincorporated herein by reference in their entirety for all purposes.

STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSOREDRESEARCH AND DEVELOPMENT

[0002] This work was supported by United States Public Health Serviceand National Heart, Lung, and Blood Institute Grants HL30568 andHL34343. The Government of the United States of America may have certainrights in this invention.

FIELD OF THE INVENTION

[0003] This invention relates to the field of atherosclerosis. Inparticular, this invention pertains to the identification of a class ofpeptides that are orally administrable and that ameliorate one or moresymptoms of atherosclerosis.

BACKGROUND OF THE INVENTION

[0004] Cardiovascular disease is a leading cause of morbidity andmortality, particularly in the United States and in Western Europeancountries. Several causative factors are implicated in the developmentof cardiovascular disease including hereditary predisposition to thedisease, gender, lifestyle factors such as smoking and diet, age,hypertension, and hyperlipidemia, including hypercholesterolemia.Several of these factors, particularly hyperlipidemia, andhypercholesteremia (high blood cholesterol concentrations) provide asignificant risk factor associated with atherosclerosis.

[0005] Cholesterol is present in the blood as free and esterifiedcholesterol within lipoprotein particles, commonly known aschylomicrons, very low density lipoprotein (VLDLs), low densitylipoprotein (LDLs), and high density lipoprotein (HDLs). Concentrationof total cholesterol in the blood is influenced by (1) absorption ofcholesterol from the digestive tract, (2) synthesis of cholesterol fromdietary constituents such as carbohydrates, proteins, fats and ethanol,and (3) removal of cholesterol from blood by tissues, especially theliver, and subsequent conversion of the cholesterol to bile acids,steroid hormones, and biliary cholesterol.

[0006] Maintenance of blood cholesterol concentrations is influenced byboth genetic and environmental factors. Genetic factors includeconcentration of rate-limiting enzymes in cholesterol biosynthesis,concentration of receptors for low density lipoproteins in the liver,concentration of rate-limiting enzymes for conversion of cholesterolsbile acids, rates of synthesis and secretion of lipoprotein and genderof person. Environmental factors influencing the hemostasis of bloodcholesterol concentration in humans include dietary composition,incidence of smoking, physical activity, and use of a variety ofpharmaceutical agents. Dietary variables include amount and type of fat(saturated and polyunsaturated fatty acids), amount of cholesterol,amount and type of fiber, and perhaps amounts of vitamins such asvitamin C and D and minerals such as calcium.

[0007] Epidemiological studies show an inverse correlation of highdensity lipoprotein (HDL) and apolipoprotein (apo) A-I levels with theoccurrence of atherosclerotic events (Wilson et al. (1988)Arteriosclerosis 8: 737-741). Injection of HDL into rabbits fed anatherogenic diet has been shown to inhibit atherosclerotic lesionformation (Badimon et al. (1990) J. Clin. Invest. 85: 1234-1241).

[0008] Human apo A-I has been a subject of intense study because of itsanti-atherogenic properties. Exchangeable apolipoproteins, including apoA-I, possess lipid-associating domains (Brouillette and Anantharamaiah(1995) Biochim. Biophys. Acta 1256:103-129; Segrest et al. (1974) FEBSLett. 38: :247-253). Apo A-I has been postulated to possess eight tandemrepeating 22 mer sequences, most of which have the potential to formclass A amphipathic helical structures (Segrest et al. (1974) FEBS Lett.38: :247-253). Characteristics of the class A amphipathic helix includethe presence of positively charged residues at the polar-nonpolarinterface and negatively charged residues at the center of the polarface (Segrest et al. (1974) FEBS Lett. 38: 247 253; Segrest et al.(1990) Proteins: Structure, Function, and Genetics 8: 103-117). Apo A-Ihas been shown to strongly associate with phospholipids to formcomplexes and to promote cholesterol efflux from cholesterol-enrichedcells. The delivery and maintenance of serum levels of apo A-I toeffectively mitigate one or more symptoms of atherosclerosis hasheretofore proven elusive.

SUMMARY OF THE INVENTION

[0009] This invention provides novel peptides administration of whichmitigate one or more symptoms of atherosclerosis. In particular, it wasa discovery of this invention that peptides comprising a class Aamphipathic helix when formulated with “D” amino acid residue(s) and/orhaving protected amino and carboxyl termini can be orally administeredto an organism, are readily taken up and delivered to the serum, and areeffective to mitigate one or more symptoms of atherosclerosis.

[0010] Thus, in one embodiment, this invention provides a peptide thatameliorates a symptom of atherosclerosis, where the peptide ranges inlength from about 10 to about 30 amino acids, comprises at least oneclass A amphipathic helix, comprises at least one “D” amino acidresidue, protects a phospholipid against oxidation by an oxidizingagent, and is not the D-18A peptide (e.g.D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F (SEQ ID NO: 1) having all D formamino acid residues). In particularly preferred embodiments, the peptidefurther comprises a protecting group coupled to the amino and/orcarboxyl terminus. Preferred protecting groups include, but are notlimited to acetyl, amide, and 3 to 20 carbon alkyl groups, Fmoc, t-boc,9-fluoreneacetyl group, 1-fluorenecarboxylic group, 9-florenecarboxylicgroup, 9-fluorenone-1-carboxylic group, benzyloxycarbonyl, Xanthyl(Xan), Trityl (Trt), 4-methyltrityl (Mtt), 4-methoxytrityl (Mmt),4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr), Mesitylene-2-sulphonyl(Mts), 4,4-dimethoxybenzhydryl (Mbh),Tosyl (Tos), 2,2,5,7,8-pentamethylchroman-6-sulphonyl (Pmc), 4-methylbenzyl (MeBzl), 4-methoxybenzyl(MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl), Benzoyl (Bz),3-nitro-2-pyridinesulphenyl (Npys),1-(4,4-dimentyl-2,6-diaxocyclohexylidene)ethyl (Dde), 2,6-dichlorobenzyl(2,6-DiCl-Bzl), 2-chlorobenzyloxycarbonyl (2-Cl-Z),2-bromobenzyloxycarbonyl (2-Br-Z), Benzyloxymethyl (Bom),t-butoxycarbonyl (Boc), cyclohexyloxy (cHxO),t-butoxymethyl (Bum),t-butoxy (tBuO), t-Butyl (tBu), Acetyl (Ac), and Trifluoroacetyl (TFA).In certain particularly preferred embodiments the peptide furthercomprises a first protecting group coupled to the amino terminus and asecond protecting group Coupled to the carboxyl terminus. Particularlypreferred peptides closely mimic the class A amphipathic helix of humanor mouse apo A-I. In certain embodiments, preferred peptides comprisegreater than about 50% amino acid sequence identity with the polypeptideencoded by the exon encoding a class A amphipathic helix of human ormouse apo A-I. In certain preferred embodiments, at least about 10%,preferably at least 20%, more preferably at least about 30%, sill morepreferably at least about 50%, even more preferably at least about 75%,and most preferably at least 90% and even 100% of the enantiomeric aminoacids are “D” amino acids. The peptide may be combined with apharmacologically acceptable excipient (e.g. an excipient suitable fororal administration to a mammal).

[0011] In certain particularly preferred embodiments, the peptidecomprises one or more of the following amino acid sequences:D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 2),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 3),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 4),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 5),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 6),D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 7),D-W-F-K-A-F-Y-D-K-F-F-E K-F-K-E-F-F- (SEQ ID NO: 8),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 9),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 10),D-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 11),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 12),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 13),E-W-L-K-L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 14),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 15),E-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 16),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 17),E-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 18),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 19), E-W-LK-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 20),A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 21),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 22),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 23),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 24),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 25),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 26),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 27),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 28),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 29), A-F-Y-D-K-V-A-E-K-F-K-EF-F- (SEQ ID NO: 30), K-A-F-Y-D-K-V-F-E-K-F-K-E-F- (SEQ ID NO: 31),L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 32),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 33),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 34),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 35),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 36),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 37),A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 38),D-W-L-K-A-L-Y-D-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 39),D-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 40),D-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F (SEQ ID NO: 41),E-W-L-K-A-L-Y-E-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 42),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 43),E-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 44),E-W-L-K-A-F-Y-E-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 45),E-W-L-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 46),E-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 47),D-F-L-K-A-W-Y-D-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 48), E-F-L-K-AW-Y-E-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 49),D-F-W-K-A-W-Y-D-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 50),E-F-W-K-A-W-Y-E-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 51),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 52),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 53),E-K-L-K-A-F-Y-E-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 54),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 55),D-W-L-K-A-F-V-D-K-F-A-E-K-F-K-E-A-Y- (SEQ ID NO: 56),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 57),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 58),E-W-L-K-A-F-V-Y-E-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 59),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 60),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 61),D-W-L-K-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 62),E-W-L-K-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 63),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 64),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 65),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 66),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 67),D-W-L-K-A-F-Y-D-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 68),E-W-L-K-A-F-Y-E-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 69),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 70),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 71),D-W-L-R-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 72),E-W-L-R-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 73),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 74),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 75),D-W-L-R-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 76),E-W-L-R-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 77),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 78),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F(SEQ ID NO: 79),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 80),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-P-D-K-L-K-A-F-Y-D-K-V-F-E-W-L-K-E-A-F(SEQ ID NO: 81),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-P-D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L(SEQ ID NO: 82),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F(SEQ ID NO: 83),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-P-D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F(SEQ ID NO: 84),D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F(SEQ ID NO: 85), truncations of the above sequences, multimericcombinations (e.g. preferably ranging from dimers to trimers, tetramers,5 mers, 8 mers, or 10 mers) of the above sequences, conservativesubstitutions of the above sequences, and/or the above sequencescomprising amino acid analogs. The enantiomeric amino acids of suchsequences preferably comprise at least one “D” amino acid. In certainpreferred embodiments, at least 50%, more preferably at lease 75%, andmost preferably at least 90% and even 100% of the enantiomeric aminoacids are “D” amino acids as described herein. Such peptides can alsoinclude a protecting group (e.g., amide, acetyl, propeonyl, and a 3 to20 carbon alkyl, etc.) coupled to the amino or carboxyl terminus. Incertain embodiments, the protecting group coupled to the carboxylterminus is an amide. In certain embodiments, the protecting groupcoupled to the amino terminus is an acetyl, a propeonyl, or a 3 to 20carbon alkyl. Certain peptides comprise both a carboxyl- and anamino-terminus protecting group. In one such embodiment, the aminoterminus protecting group is a protecting group selected from the groupconsisting of acetyl, propeonyl, and a 3 to 20 carbon alkyl; and thecarboxyl terminal protecting group is an amide.

[0012] In certain embodiments, the peptide is one that protects aphospholipid against oxidation by an oxidizing agent selected from thegroup consisting of lipids such as hydrogen peroxide, 13(S)-HPODE,15(S)-HPETE, HPODE, HPETE, HODE, and IIETE. The phospholipid can be aphospholipid selected from the group consisting of1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine (SAPE).Thus the peptide prevents the formation of lipids such as oxidized1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (Ox-PAPC),1-palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine (POVPC),1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine (PGPC),1-palmitoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylcholine (PEIPC),oxidized 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine(Ox-SAPC),1-stearoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine(SOVPC),1-stearoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine (SGPC),1-stearoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylcholine (SEIPC),oxidized 1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine(Ox-SAPE), 1-stearoyl-2-oxovaleroyl-sn-glycero-3-phosphorylethanolamine(SOVPE), 1-stearoyl-2-glutaroyl-sn-glycero-3-phosphorylethanolamine(SGPE), and1-stearoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylethanolamine(SEIPE).

[0013] In another embodiment, this invention provides a composition,suitable for oral administration, that ameliorates a symptom ofatherosclerosis. The composition comprises a peptide that is a human apoA-I peptide or fragment thereof comprising a class A amphipathic helix,or an analogue of a human apo A-I peptide wherein said peptide has afirst protecting group attached to an amino terminal and a secondprotecting group attached to a carboxyl terminal and further whereinsaid peptide comprises a plurality of D amino acid residues. Theprotecting groups include, but are not limited to the protecting groupsdescribed herein. In certain embodiments, more than half, morepreferably more than 80%, and most preferably more than 90% or even allof the enantiomeric amino acids comprising the peptide are D aminoacids. The composition can further comprise a pharmaceuticallyacceptable excipient (e.g., an excipient suitable for oraladministration or an excipient suitable for injection). Preferredpeptides are capable of protecting a phospholipid [e.g.,1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine (SAPE)]from oxidization by an oxidizing agent (e.g. hydrogen peroxide,13(S)-HPODE, 15(S)-HPETE, HPODE, HPETE, HODE, and HETE). Thus thepeptide prevents the formation of oxidized1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (Ox-PAPC),1-palmitoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine (POVPC),1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine (PGPC),1-palmitoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylcholine (PEIPC),oxidized 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine(Ox-SAPC), 1-stearoyl-2-oxovaleroyl-sn-glycero-3-phosphorylcholine(SOVPC), 1-stearoyl-2-glutaroyl-sn-glycero 3-phosphorylcholine (SGPC),1-stearoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylcholine (SEIPC),oxidized 1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine(Ox-SAPE), 1-stearoyl-2-oxovaleroyl-sn-glycero-3-phosphorylethanolamine(SOVPE), 1-stearoyl-2-glutaroyl-sn-glycero-3-phosphorylethanolamine(SGPE), and1-stearoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylethanolamine(SEIPE).

[0014] This invention also provides methods of ameliorating a symptom ofatherosclerosis. The methods comprise administering to an organism (e.g.human or non-human mammal) one or more of the peptides described herein.In particularly preferred embodiments, such peptides comprise aplurality of “D” amino acids and/or are protected as described herein.The peptide is preferably orally administered to the organism and theorganism is preferably an organism diagnosed as having or as at risk forone or more symptoms of atherosclerosis. In certain embodiments, thepeptide can be provided as an isolated peptide or combined with apharmacological excipient as described herein. The administration ispreferably at a dosage sufficient to ameliorate one or more symptoms ofatherosclerosis and/or to significantly reduce the likelihood ofoccurrence of one or more symptoms of atherosclerosis.

[0015] In still another embodiment, this invention provides a kit forameliorating a symptom of atherosclerosis. Preferred kits include acontainer containing one or more of the peptides described herein. Thepeptides preferably comprise a plurality of “D” amino acids and/or areprotected as described herein. In certain embodiments, the kit canoptionally further include a pharmaceutically acceptable excipientand/or the peptide is provided combined with a with a pharmaceuticallyacceptable excipient (e.g. in a unit dosage formulation). Preferred kitsprovided the peptide(s) as a unit dosage formulation is for oraladministration. The kits also, optionally, include instructionalmaterials teaching the use of said peptide for ameliorating one or moresymptoms of atherosclerosis and/or for reducing the likelihood ofoccurrence of one or more symptoms of atherosclerosis.

[0016] In certain embodiments, this invention excludes any one or morepeptides disclosed in U.S. Pat. No. 4,643,988 and/or in Garber et al.(1992) Arteriosclerosis and Thrombosis, 12: 886-894. In certainembodiments this invention excludes any one or more peptides disclosedin U.S. Pat. No. 4,643,988 and/or in Garber et al (1992) that weresynthesized with all enantiomeric amino acids being L amino acids orsynthesized with D amino acids where the peptides are blocking groups.In certain embodiments, this invention excludes peptides having theformula A₁-B₁-B₂-C₁-D-B₃-B₄-A₂-C₂-B₅-B₆-A₃-C₃-B₇-C₄-A₄-B₈-B₉ (SEQ ID NO:87) wherein A₁, A₂, A₃ and A₄ are independently aspartic acid orglutamic acid, or homologues or analogues thereof; B₁, B₂, B₃, B₄, B₅,B₆, B₇, B₈ and B₉ are independently tryptophan, phenylalanine, alanine,leucine, tyrosine, isoleucine, valine or α-naphthylalanine, orhomologues or analogues thereof; C₁, C₂, C₃ and C₄ are independentlylysine or arginine, and D is serine, threonine, alanine, glycine,histidine, or homologues or analogues thereof; provided that, when A₁and A₂ are aspartic acid, A₃ and A₄ are glutamic acid, B₂ and B₉ areleucine, B₃ and B₇ are phenylalanine, B₄ is tyrosine, B₅ is valine, B₆,B₈, and D are alanine, and C₁, C₂, C₃ and C₄ are lysine, B₁ is nottryptophan.

[0017] Definitions.

[0018] The terms “polypeptide”, “peptide” and “protein” are usedinterchangeably herein to refer to a polymer of amino acid residues. Theterms apply to amino acid polymers in which one or more amino acidresidues is an artificial chemical analogue of a corresponding naturallyoccurring amino acid, as well as to naturally occurring amino acidpolymers.

[0019] The term “class A amphipathic helix” refers to a proteinstructure that forms an α-helix producing a segregation of a polar andnonpolar faces with the positively charged residues residing at thepolar-nonpolar interface and the negatively charged residues residing atthe center of the polar face (see, e.g., ”Segrest et al. (1990)Proteins: Structure, Function, and Genetics 8: 103-117).

[0020] The term “ameliorating” when used with respect to “amelioratingone or more symptoms of atherosclerosis” refers to a reduction,prevention, or elimination of one or more symptoms characteristic ofatherosclerosis and/or associated pathologies. Such a reductionincludes, but is not limited to a reduction or elimination of oxidizedphospholipids, a reduction in atherosclerotic plaque formation andrupture, a reduction in clinical events such as heart attack, angina, orstroke, a decrease in hypertension, a decrease in inflammatory proteinbiosynthesis, reduction in plasma cholesterol, and the like.

[0021] The term “enantiomeric amino acids” refers to amino acids thatcan exist in at least two forms that are nonsuperimposable mirror imagesof each other. Most amino acids (except glycine) are enantiomeric andexist in a so-called L-form (L amino acid) or D-form (D amino acid).Most naturally occurring amino acids are “L” amino acids. The terms “Damino acid” and “L amino acid” are used to refer to absoluteconfiguration of the amino acid, rather than a particular direction ofrotation of plane-polarized light. The usage herein is consistent withstandard usage by those of skill in the art.

[0022] The term “protecting group” refers to a chemical group that, whenattached to a functional group in an amino acid (e.g. a side chain, analpha amino group, an alpha carboxyl group, etc.) blocks or masks theproperties of that functional group. Preferred amino-terminal protectinggroups include, but are not limited to acetyl, or amino groups. Otheramino-terminal protecting groups include, but are not limited to alkylchains as in fatty acids, propeonyl, formyl and others. Preferredcarboxyl terminal protecting groups include, but are not limited togroups that form amides or esters.

[0023] The phrase “protect a phospholipid from oxidation by an oxidizingagent” refers to the ability of a compound to reduce the rate ofoxidation of a phospholipid (or the amount of oxidized phospholipidproduced) when that phospholipid is contacted with an oxidizing agent(e.g. hydrogen peroxide, 13-(S)-HPODE, 15-(S)-HPETE, HPODE, HPETE, HODE,HETE, etc.).

[0024] The terms “low density lipoprotein” or “LDL” is defined inaccordance with common usage of those of skill in the art. Generally,LDL refers to the lipid-protein complex which when isolated byultracentrifugation is found in the density range d=1.019 to d=1.063.

[0025] The terms “high density lipoprotein” or “HDL” is defined inaccordance with common usage of those of skill in the art. Generally“HDL” refers to a lipid-protein complex which when isolated byultracentrifugation is found in the density range of d=1.063 to d=1.21.

[0026] The term “Group I HDL” refers to a high density lipoprotein orcomponents thereof (e.g. apo A-I, paraoxonase, platelet activatingfactor acetylhydrolase, etc.) that reduce oxidized lipids (e.g. in lowdensity lipoprotein) or that protect oxidized lipids from oxidation byoxidizing agents.

[0027] The term “Group II HDL” refers to an HDL that offers reducedactivity or no activity in protecting lipids from oxidation or inrepairing (e.g. reducing) oxidized lipids.

[0028] The term “HDL component” refers to a component (e.g. molecules)that comprises a high density lipoprotein (HDL). Assays for HDL thatprotect lipids from oxidation or that repair (e.g. reduce oxidizedlipids) also include assays for components of HDL (e.g. apo A-I,paraoxonase, platelet activating factor acetylhydrolase, etc.) thatdisplay such activity.

[0029] The term “human apo A-I peptide” refers to a full-length humanapo A-I peptide or to a fragment or domain thereof comprising a class Aamphipathic helix.

[0030] A “monocytic reaction” as used herein refers to monocyte activitycharacteristic of the “inflammatory response” associated withatherosclerotic plaque formation. The monocytic reaction ischaracterized by monocyte adhesion to cells of the vascular wall (e.g.cells of the vascular endothelium), and/or chemotaxis into thesubendothelial space, and/or differentiation of monocytes intomacrophages.

[0031] The term “absence of change” when referring to the amount ofoxidized phospholipid refers to the lack of a detectable change, morepreferably the lack of a statistically significant change (e.g. at leastat the 85%, preferably at least at the 90%, more preferably at least atthe 95%, and most preferably at least at the 98% or 99% confidencelevel). The absence of a detectable change can also refer to assays inwhich oxidized phospholipid level changes, but not as much as in theabsence of the protein(s) described herein or with reference to otherpositive or negative controls.

[0032] The following abbreviations are used herein: PAPC:L-α-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine; POVPC:1-palmitoyl-2-(5-oxovaleryl)-sn-glycero-3-phosphocholine; PGPC:1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine; PEIPC:1-palmitoyl-2-(5,6-epoxyisoprostane E₂)-sn-glycero-3-phsophocholine;ChC18:2: cholesteryl linoleate; ChC18:2-OOH: cholesteryl linoleatehydroperoxide; DMPC: 1,2-ditetradecanoyl-rac-glycerol-3-phosphocholine;PON: paraoxonase; HPF: Standardized high power field; PAPC:L-α-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine; POVPC:1-palmitoyl-2-(5-oxovaleryl)-sn-glycero-3-phosphocholine; PGPC:1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine; PEIPC:1-palmitoyl-2-(5,6-epoxyisoprostane E₂)-sn-glycero-3-phosphocholine;PON: paraoxonase; BL/6: C57BL/6J; C3H:C3H/HeJ.

[0033] The term “conservative substitution” is used in reference toproteins or peptides to reflect amino acid substitutions that do notsubstantially alter the activity (specificity (e.g. for lipoprotein)) orbinding affinity (e.g. for lipids or lipoproteins)) of the molecule.Typically conservative amino acid substitutions involve substitution oneamino acid for another amino acid with similar chemical properties (e.g.charge or hydrophobicity). The following six groups each contain aminoacids that are typical conservative substitutions for one another: 1)Alanine (A), Serine (S), Threonine (T); 2) Aspartic acid (D), Glutamicacid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K);5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); and 6)Phenylalanine (F), Tyrosine (Y), Tryptophan (W).

[0034] The terms “identical” or percent “identity,” in the context oftwo or more nucleic acids or polypeptide sequences, refer to two or moresequences or subsequences that are the same or have a specifiedpercentage of amino acid residues or nucleotides that are the same, whencompared and aligned for maximum correspondence, as measured using oneof the following sequence comparison algorithms or by visual inspection.With respect to the peptides of this invention sequence identity isdetermined over the full length of the peptide.

[0035] For sequence comparison, typically one sequence acts as areference sequence, to which test sequences are compared. When using asequence comparison algorithm, test and reference sequences are inputinto a computer, subsequence coordinates are designated, if necessary,and sequence algorithm program parameters are designated. The sequencecomparison algorithm then calculates the percent sequence identity forthe test sequence(s) relative to the reference sequence, based on thedesignated program parameters.

[0036] Optimal alignment of sequences for comparison can be conducted,e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl.Math. 2:482 (1981), by the homology alignment algorithm of Needleman &Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity methodof Pearson & Lipman (1988) Proc. Natl. Acad. Sci. USA 85:2444, bycomputerized implementations of these algorithms (GAP, BESTFIT, FASTA,and TFASTA in the Wisconsin Genetics Software Package, Genetics ComputerGroup, 575 Science Dr., Madison, Wis.), or by visual inspection (seegenerally Ausubel et al., supra).

[0037] One example of a useful algorithm is PILEUP. PILEUP creates amultiple sequence alignment from a group of related sequences usingprogressive, pairwise alignments to show relationship and percentsequence identity. It also plots a tree or dendogram showing theclustering relationships used to create the alignment. PILEUP uses asimplification of the progressive alignment method of Feng & Doolittle(1987) J. Mol. Evol. 35:351-360. The method used is similar to themethod described by Higgins & Sharp (1989) CABIOS 5: 151-153. Theprogram can align up to 300 sequences, each of a maximum length of 5,000nucleotides or amino acids. The multiple alignment procedure begins withthe pairwise alignment of the two most similar sequences, producing acluster of two aligned sequences. This cluster is then aligned to thenext most related sequence or cluster of aligned sequences. Two clustersof sequences are aligned by a simple extension of the pairwise alignmentof two individual sequences. The final alignment is achieved by a seriesof progressive, pairwise alignments. The program is run by designatingspecific sequences and their amino acid or nucleotide coordinates forregions of sequence comparison and by designating the programparameters. For example, a reference sequence can be compared to othertest sequences to determine the percent sequence identity relationshipusing the following parameters: default gap weight (3.00), default gaplength weight (0.10), and weighted end gaps.

[0038] Another example of algorithm that is suitable for determiningpercent sequence identity and sequence similarity is the BLASTalgorithm, which is described in Altschul et al. (1990) J. Mol. Biol.215: 403-410. Software for performing BLAST analyses is publiclyavailable through the National Center for Biotechnology Information(http://www.ncbi.nlm.nih.gov/). This algorithm involves firstidentifying high scoring sequence pairs (HSPs) by identifying shortwords of length W in the query sequence, which either match or satisfysome positive-valued threshold score T when aligned with a word of thesame length in a database sequence. T is referred to as the neighborhoodword score threshold (Altschul et al, supra). These initial neighborhoodword hits act as seeds for initiating searches to find longer HSPscontaining them. The word hits are then extended in both directionsalong each sequence for as far as the cumulative alignment score can beincreased. Cumulative scores are calculated using, for nucleotidesequences, the parameters M (reward score for a pair of matchingresidues; always >0) and N (penalty score for mismatching residues;always <0). For amino acid sequences, a scoring matrix is used tocalculate the cumulative score. Extension of the word hits in eachdirection are halted when: the cumulative alignment score falls off bythe quantity X from its maximum achieved value; the cumulative scoregoes to zero or below, due to the accumulation of one or morenegative-scoring residue alignments; or the end of either sequence isreached. The BLAST algorithm parameters W, T, and X determine thesensitivity and speed of the alignment. The BLASTN program (fornucleotide sequences) uses as defaults a wordlength (W) of 11, anexpectation (E) of 10, M=5, N=4, and a comparison of both strands. Foramino acid sequences, the BLASTP program uses as defaults a wordlength(W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (seeHenikoff & Henikoff (1989) Proc. Natl. Acad. Sci. USA 89:10915).

[0039] In addition to calculating percent sequence identity, the BLASTalgorithm also performs a statistical analysis of the similarity betweentwo sequences (see, e.g., Karlin & Altschul (1993) Proc. Natl. Acad.Sci. USA, 90: 5873-5787). One measure of similarity provided by theBLAST algorithm is the smallest sum probability (P(N)), which providesan indication of the probability by which a match between two nucleotideor amino acid sequences would occur by chance. For example, a nucleicacid is considered similar to a reference sequence if the smallest sumprobability in a comparison of the test nucleic acid to the referencenucleic acid is less than about 0.1, more preferably less than about0.01, and most preferably less than about 0.001.

[0040] The term “D-18A peptide” refers to a peptide having the sequence:D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F (SEQ ID NO: 1) where all of theenantiomeric amino acids are D form amino acids.

BRIEF DESCRIPTION OF THE DRAWINGS

[0041]FIGS. 1A, 1B, 1C, and 1D show the association of ¹⁴C-D-5F withblood components in an ApoE null mouse. ApoA-I mimetic peptide D-5Flabeled with ¹⁴C amino acids was administered by oral gavage to apo Edeficient mice (n=5) or incubated with their plasma in vitro. Fastingblood was collected 6 hrs after gavage and ¹⁴C association with blood,plasma, and lipoproteins determined.

[0042]FIGS. 2A and 2B illustrate that orally administered D peptide isactive. ApoA-I mimetic peptides D-5F and L-5F (100 μg per animal) wereadministered to LDL. receptor null mice (n=5) by oral gavage. Blood wascollected after 6 hrs, LDL and HDL were isolated by gel filtration(FPLC) and examined in the artery wall model system for HDL protectivecapacity (FIG. 2A) and LDL resistance (FIG. 2B) to oxidation bydetermining monocyte chemotactic activity generated. As seen. D-5F butnot L-5F rendered the HDL markedly more protective and LDL after D-5Fbecame highly resistant to oxidation.

[0043]FIGS. 3A and 3B show the plasma concentration of D vs L peptideafter gavage. ApoA-I mimetic peptides D-4F (FIG. 3B) and L-4F (FIG. 3B)were labeled with ¹²⁵I and administered by oral gavage to LDL receptornull mice (n=4). Blood was collected after 3 hrs, plasma fractionated byFPLC and radioactivity determined in the eluted fractions. Less than 15%of the L peptide eluted as intact 18 mer whereas more than 70% of theD-4F was intact. These studies demonstrate that the D peptide isdramatically more resistant to degradation in vivo compared with the Lpeptide.

[0044]FIG. 4 illustrates the absence of antibody to D-4F in treatedmice. No antibody (white precipitation line) to D-4F was detected in LDLreceptor null mouse plasma following 6 weeks of treatment with peptideat 5 mg per day (lower panel). The positive control (upper panel) showsthe presence of a precipitation line for apoA-I in mouse plasma. Upperpanel: Center: rabbit anti ApoA-I and periphery: plasma from D-4F mice.Lower panel: Center: Plasma from LDL R−/−mice treated with D 4F, andPeriphery: Pure D-4F peptide at 0 to 80 μg.

[0045]FIG. 5 shows the incidence of fatty streak lesions in the aorticroot of LDL receptor null mice on a Western Diet. Groups of LDL receptornull mice were placed on a Western type diet and were given orally,vehicle (Control) (n=9) or peptide D-4F (n=6), twice daily for 6 weeks.The mice were subsequently sacrificed, aortic arch fixed and sectionedand fatty streak lesions quantified. The mice receiving D-4F had an 81%reduction in lesion area (p<0.01).

[0046]FIGS. 6A, 6B, and 6C illustrate the plasma distributions ofpeptide 5F or apo A-I following intraperitoneal injection. Human apoA-I, mouse apo A-I, and peptide 5F were labeled with ¹²⁵I and injectedintraperitoneally into C57BL/6 mice that had been fed the atherogenicdiet for at least three weeks. Samples were taken during the kineticstudies described in Table 3. Representative samples were analyzed bythe CLiP method, and fractions were collected for determination ofradioactivity. The elution volume was based on the column pump rateonly; the volume contributed by the enzymatic reagent pump wasneglected. Data shown are cholesterol (as absorbance at 500 nm inarbitrary units; solid lines) and radioactivity (in counts per minute;dashed lines). FIGS. are 6A: human apo A-I (one hour followinginjection); 6B: mouse apo A-I (one hour), 6C: 5F (1.5 hours)

[0047]FIGS. 7A and 7B illustrate the interaction of mouse lipoproteinwith human artery wall cells. LDL and HDL were isolated by FPLC from theplasma of mice fed the atherogenic diet and injected with vehicle (PBS),or with peptide 5F at 20 μg/mouse/day. The cocultures were treatedwithout (No Addition) or with human LDL (hLDL) at 200 μg/ml LDL protein,or mouse LDL (MoLDL) at 200 μg/ml or with 200 μg/ml human LDL+human HDL(hHDL) at 350 μg/ml of HDL protein or mouse HDL (MoHDL) at 300 μg/ml.The cocultures were incubated with the above additions for 8 hrs at 37°C. in the presence of 10% lipoprotein deficient serum (LPDS). Thesupernatants were collected and analyzed for Auerbach lipidhydroperoxide equivalents (FIG. 7A). The cocultures were then washed andincubated with fresh culture medium without serum or LPDS for anadditional 8 hrs. The conditioned medium was collected and analyzed formonocyte chemotactic activity (FIG. 7B). A no cell blank (No Cell Blank)is included in both figures for comparison.

[0048]FIG. 8 shows mean lesion cross-sectional areas. Data shownrepresent the mean lesion cross-sectional area for each animal (◯ andthe mean±SEM of all animals in each group () with error bars.Abbreviations: PBS, mice fed the atherogenic diet and injected dailywith 200 μl phosphate-buffered saline; 5F, mice fed the atherogenic dietand injected daily with 20 μg of 5F in 200 μl PBS; MoAI, mice fed theatherogenic diet and injected daily with 50 μg of mouse apo A-I in 200μl PBS. *=p<0.002 as determined by two-tailed t-test. A significantdifference was also shown using one way analysis of variance on ranks(p<0.001).

[0049]FIG. 9 shows that both the D and L isomers of apo A-I peptidemimetics prevent monocyte chemotactic activity induced by mildlyoxidized LDL in vitro. Medium alone (LDL, NO CELLS or CELLS, NO LDL),control LDL from normal subjects at 250 μg/ml (LDL), and LDL pluscontrol HDL from normal subjects at 350 μg/ml (+HDL). Other cocultureswere incubated with the control LDL together with varying amounts(micrograms shown on the abscissa) of either D-2F, or L-2F (third panelfrom the left, 2F) or D-37-pA or L-37pA (last panel on the right, 37pA).The data represent mean±SD of values obtained from quadruplicatecocultures. Values for HDL or added peptides were all significantlydifferent from LDL alone (first panel on the left) at the level ofp<0.01.

[0050]FIGS. 10A and 10B illustrate that feeding mice ApoA-I peptidemimetics of this invention renders red cells resistant to n vitro lysis.FIGS. 10A and 10B show the results of in vitro red cell lysis assay at18 hours (FIG. 10A) and at 48 hours (FIG. 10B). The asterisks reflectthe presence of a significant difference (p<0001) between the red celllysis for animals that received the vehicle versus those that receivedthe peptides.

[0051]FIG. 11 shows that feeding mice ApoA-I mimetic D peptides of thisinvention renders circulating LDL resistant to oxidation. Groups of LDLreceptor-deficient mice (n=3) were administered the D-peptides or thesaline vehicle by gavage. Each animal was given 100 μl of saline, 100μg/100 μl of peptide D-2F or peptide D-37pA. Blood was collected fromretroorbital sinus under mild anesthesia 17 hrs later. LDL was isolatedfrom plasma by FPLC. Cocultures of artery wall cells were incubated withmedium alone (NO ADDITION), control LDL from normal subjects (LDL), LDLplus control HDL from normal subjects (+HDL). Other cocultures wereincubated with murine LDL following gavage with saline (SALINE LDL),with D-2F (D-2F LDL) or with D-37pA peptide (D-37pA LDL). The cocultureswere incubated for 4 hrs at 37° C. in the presence of 10% LPDS. Thesupernatants were then discarded, the cocultures were washed andincubated with culture medium without serum or LPDS for an additional 4hrs. This conditioned medium was collected and analyzed for monocytechemotactic activity. The values are mean±SD of quadruplicatecocultures. The asterisks indicate p<0.001.

[0052]FIG. 12 illustrates the results of a chemotaxis assay comparinglipoproteins from mice given the D-form and or L-form peptides bygavage.

[0053]FIG. 13A illustrates the results of a chemotaxis assay comparingcontrol HDL and HDL from mice given the D-peptide by gavage. FIG. 13Billustrates the results of a chemotaxis assay comparing LDL and VLDL/IDLfrom mice given the D-peptide by gavage.

[0054]FIGS. 14A and 14B show electrophoresis of 2F indicating itsself-association. FIG. 14A: SDS PAGE (18%) of 2F. Lane 1 shows themolecular weight standard and line 2 shows the band corresponding to 2F(molecular weight is 2242) moving slightly lower than the lowestmolecular weight standard (3.5-2.5 kDa). FIG. 14B: Non-denaturing PAGE(4-12%) showing the mobilities of 100 μg/ml (lane 2) and 250 μg/ml(lane 1) of 2F indicating self-association in solution. Lane 3 shows themobility of the high molecular weight standard.

[0055]FIG. 15 shows that the homologous series of peptides stabilize thehex-phase transition of DiPoPE bilayers. Shift in T_(H) of DiPoPE as afunction of the mole fraction of added peptide. Measured by DSC at aheating scan rate of 37°/h.  2F; ◯ 3F³; ▪ 4F; □ 5F; ▾ 6F; ▴ 7F; Δ apoA-I.

[0056]FIG. 16: Relative right angle light scattering monitoring of thedissolution of EPC MLVs by homologous series of peptides as a functionof time. A representative EPC MLV clarification curve is shown for eachof the homologous peptides. An equimolar concentration of peptide andEPC was used (105 μM). Both excitation and emission wavelengths were 400nm. Triton X-100 achieved complete dissolution at a final concentrationof 1 mM. --EPC; -◯-2F; -▪-3F³; -□-3F¹⁴; -▴-4F; -Δ-5F; -▾-6F; -∇-7F;-⋄-human apo A-I; -♦-Triton X-100.

[0057]FIG. 17 illustrates LCAT activating ability of homologouspeptides. Histograms representing activation of LCAT by the F-peptides.LCAT activity was measured using small unilamellar vesicles ofEPC-cholesterol and the activity is represented as a percentage comparedto that of apo A-I activity, where apo A-I activity is taken to be 100%.Each value represents an average value from triplicates. The peptideconcentration used was 20 μg/ml.

[0058]FIG. 18 shows that LDL-induced monocyte chemotaxis was inhibitedby the homologous series of peptides. LDL alone or LDL incubated witheither human HDL or the homologous series of peptides was added to thehuman artery wall cell cocultures for 8 h in the presence of 10% LPDS.The supernatants were removed and the cocultures were washed withculture medium without serum or LPDS. The conditioned medium was thencollected and analyzed for monocyte chemotactic activity. The datarepresent mean±SEM values (n=9 in each case). By pair-wise comparisonswith LDL all peptides except the 3F peptides were significantly moreeffective (at least p<0.001, signified by ‘†’ and *). Comparisonsbetween all peptides were analyzed by one-way ANOVA. The asteriskindicates that peptides 4F, 5F and 6F were significantly more effectivethan the homologues 2F and 7F (p<0.05 by Duckett comparison). Thebracket indicates no significant difference in the ability to inhibitLDL-induced chemotaxis among these three peptides.

[0059]FIG. 19 shows that Influenza A infection causes an increase inhepatic oxidized phospholipids two days after infection. C57BL/6 mice ona chow diet were infected with a dose of influenza A virus intranasallysuch that no viremia resulted as described by Van Lenten et al. (2001)Circulation, 103: 2283-2288. Zero, 2, 3, 5, 7, and 9 days afterinfection the livers were removed and oxidized phospholipid contentdetermined by ESI-MS.

[0060]FIG. 20 shows that D-4F prevents the decrease in paraoxonaseactivity after Influenza A infection. Some of the mice described in FIG.19 were injected intraperitoneally with 20 μg daily of D-4F and theothers were injected with phosphate buffered saline (PBS). Paraoxonaseactivity (PON) was measured in the plasma at zero, 2, 7, and 9 daysafter infection.

[0061]FIG. 21 shows that D-4F prevents the induction of oxidizedphospholipids in aortas of mice infected with Influenza A virus. Some ofthe mice described in FIG. 19 were injected intraperitoneally with 20 μgdaily of D-4F and the others were injected with phosphate bufferedsaline (PBS). The aortas of the mice were harvested at days zero, 2, 7and 9 days after infection and oxidized phospholipid content wasdetermined by ESI-MS.

[0062]FIGS. 22A through 22C show that, after oral administration, D-4F,but not L-4F remains intact in the circulation of LDL receptor null miceand enhances HDL's ability to protect LDL against oxidation by humanartery wall cells and reduces LDL-induced monocyte chemotactic activity.FIG. 22A: Peptides L-4F and D-4F were radiolabeled using the lodo-beadreagent and were administered by oral gavage to LDL receptor null mice(100 μl of saline containing 100 μg of unlabeled peptide plus¹²⁵I-labeled peptide with specific activity of 11×10⁶ cpm per μg peptideper animal, n=3). Blood was collected after 4 hrs, plasma was separated,delipidated and analyzed by reverse phase HPLC as described herein. FIG.22B and 22C: Peptides L-4F and D-4F (100 μg in 100 μl of saline peranimal) were administered to LDL receptor deficient mice (n=5) by oralgavage. Blood was collect after 6 hrs, plasma HDL and LDL were isolatedby gel filtration (FPLC) and examined in the cocultures. FIG. 22B: Theability of mouse and human LDL to protect a control human LDL (hLDL)against oxidation by human artery wall cells and to inhibit LDL-inducedmonocyte chemotactic activity is shown. Human LDL at 200 μg protein/mlwas added to human artery wall cell cocultures together with human HDL(hHDL) at 350 μg protein/ml or mouse HDL (mHDL) at 100 μg cholesterol/mltaken from mice that received saline (Saline Rx) or L-4F (L-4F Rx) orD-4F (D-4F Rx) and monocyte chemotactic activity was determined asdescribed herein. FIG. 22C: The ability of mouse LDL to induce monocytechemotactic activity is shown. The assay controls are shown on the leftas in FIG. 22B. On the right, mouse LDL (mLDL) was isolated from micereceiving saline (Saline Rx) or L-4F (L4F Rx) or D-4F (D-4F Rx) and wasadded at 100 μg cholesterol/ml to the artery wall cell cocultureswithout HDL and monocyte chemotactic activity determined. Values aremean±SD) for four wells in two independent experiments.

[0063] FIGS. 23 shows that oral administration of D-4F dramaticallyreduces lesions in LDL receptor null mice on a Western diet. Groups ofLDL receptor null mice were placed on a Western diet and were given byoral gavage, 100 μl of saline alone (Saline), n=4 animals, or 100 μl ofliposomes without D-4F (Liposomes), n=5 animals, or 2.5 mg D-4F peptidein 100 μl liposomes (D-4F in liposomes), n=6 animals, twice daily for 6weeks. The mice were bled, and subsequently sacrificed, aortic rootfixed, sectioned and the extent of Oil Red O staining in fatty streaklesions quantified.

[0064]FIG. 24 shows that oral administration of D-4F dramaticallyreduces lesions in apo E null mice on a chow diet. At 4 weeks of age,D-4F was added to the drinking water of some of the apo E null mice togive a concentration of 1 mg/ml of D-4F (n=4 mice). D-4F at aconcentration of 2 mg/ml was added to the drinking water of anothergroup of mice (n=4 mice) and no peptide was added to the drinking waterof a third group of mice (n=5 mice). The mice all consumed approximately2.5 ml of water per day so that one group received no peptide (Water), asecond group received 2.5 mg D-4F/mouse/day (2.5 mg D-4F) and the thirdgroup received 5.0 mg D-4F/mouse/day (5.0 mg D-4F). All were continuedon the chow diet for 5 weeks at which time the mice were bled andsubsequently sacrificed, aortic root fixed, sectioned and the extent ofOil Red O staining in fatty streak lesions quantified.

DETAILED DESCRIPTION

[0065] I. Mitigation of a Symptom of Atherosclerosis.

[0066] This invention pertains to the discovery that synthetic peptidesdesigned to mimic the class A amphipathic helical motif (Segrest et al.(1990) Proteins: Structure, Function and Genetics 8: 103-117) are ableto associate with phospholipids and exhibit many biological propertiessimilar to human apo-A-I. In particular, it was a discovery of thisinvention that when such peptides are formulated using D amino acids,the peptides show dramatically elevated serum half-lives and,particularly when the amino and/or carboxy termini are blocked, can evenbe orally administered.

[0067] Moreover, it was a surprising discovery of this invention thatsuch D-form peptides retain the biological activity of the correspondingL-form peptide. In vivo animal studies using such D-form peptides showedeffective oral delivery, elevated serum half-life, and the ability tomitigate or prevent/inhibit one or more symptoms of artherosclerosis.

[0068] We discovered that normal HDL inhibits three steps in theformation of mildly oxidized LDL. In those studies (see, copendingapplication U.S. Ser. No. 09/541,468, filed on Mar. 31, 2000) wedemonstrated that treating human LDL in vitro with apo A-I or an apo A-Imimetic peptide (37pA) removed seeding molecules from the LDL thatincluded HPODE and HPETE. These seeding molecules were required forcocultures of human artery wall cells to be able to oxidize LDL and forthe LDL to induce the artery wall cells to produce monocyte chemotacticactivity. We also demonstrated that after injection of apo A-I into miceor infusion into humans, the LDL isolated from the mice or humanvolunteers after injection/infusion of apo A-I was resistant tooxidation by human artery wall cells and did not induce monocytechemotactic activity in the artery wall cell cocultures.

[0069] The protective function of the D peptides of this invention isillustrated in FIGS. 1 through 5. FIGS. 1A, 1B, 1C, and 1D show theassociation of 14C-D-5F with blood components in an ApoE null mouse. Itis also demonstrated herein, that HDL from mice that were fed anatherogenic diet and injected with PBS failed to inhibit the oxidationof human LDL and failed to inhibit LDL-induced monocyte chemotacticactivity in human artery wall cocultures. In contrast, HDL from mice fedan atherogenic diet and injected daily with peptides described hereinwas as effective in inhibiting human LDL oxidation and preventingLDL-induced monocyte chemotactic activity in the cocultures as wasnormal human HDL (FIGS. 2A and 2B). In addition, LDL taken from mice fedthe atherogenic diet and injected daily with PBS was more readilyoxidized and more readily induced monocyte chemotactic activity than LDLtaken from mice fed the same diet but injected with 20 μg daily ofpeptide 5F. The D peptide did not appear to be immunogenic (FIG. 4).

[0070] The in vitro responses of human artery wall cells to HDL and frommice fed the atherogenic diet and injected with a peptide according tothis invention are consistent with the protective action of shown bysuch peptides in vivo. Despite similar levels of total cholesterol,LDL-cholesterol, IDL+VLDL-cholesterol, and lower HDL-cholesterol as apercent of total cholesterol, the animals fed the atherogenic diet andinjected with the peptide had significantly lower lesion scores (FIG.5). The peptides of this invention thus prevented progression ofatherosclerotic lesions in mice fed an atherogenic diet.

[0071] Thus, in one embodiment, this invention provides methods forameliorating and/or preventing one or more symptoms of atherosclerosis.The methods preferably involve administering to an organism, preferablya mammal, more preferably a human one or more of the peptides of thisinvention (or mimetics of such peptides). The peptide(s) can beadministered, as described herein, according to any of a number ofstandard methods including, but not limited to injection, suppository,nasal spray, time-release implant, transdermal patch, and the like. Inone particularly preferred embodiment, the peptide(s) are administeredorally (e.g. as a syrup, capsule, or tablet).

[0072] The methods involve the administration of a single polypeptide ofthis invention or the administration of two or more differentpolypeptides. The polypeptides can be provided as monomers or indimeric, oligomeric or polymeric forms. In certain embodiments, themultimeric forms may comprise associated monomers (e.g. ionically orhydrophobically linked) while certain other multimeric forms comprisecovalently linked monomers (directly linked or through a linker).

[0073] While the invention is described with respect to use in humans,it is also suitable for animal, e.g. veterinary use. Thus preferredorganisms include, but are not limited to humans, non-human primates,canines, equines, felines, porcines, ungulates, largomorphs, and thelike.

[0074] The methods of this invention are not limited to humans ornon-human animals showing one or more symptom(s) of atherosclerosis(e.g. hypertension, plaque formation and rupture, reduction in clinicalevents such as heart attack, angina, or stroke, high levels of plasmacholesterol, high levels of low density lipoprotein, high levels of verylow density lipoprotein, or inflammatory proteins, etc.), but are usefulin a prophylactic context. Thus, the peptides of this invention (ormimetics thereof) may be administered to organisms to prevent theonset/development of one or more symptoms of atherosclerosis.Particularly preferred subjects in this context are subjects showing oneor more risk factors for atherosclerosis (e.g. family history,hypertension, obesity, high alcohol consumption, smoking, high bloodcholesterol, high blood triglycerides, elevated blood LDL, VLDL, IDL, orlow HDL, diabetes, or a family history of diabetes, high blood lipids,heart attack, angina or stroke, etc.).

[0075] In addition to methods of use of the atherosclerosis-inhibitingpeptides of this invention, this invention also provides the peptidesthemselves, the peptides formulated as pharmaceuticals, particularly fororal delivery, and kits for the treatment and/or prevention of one ormore symptoms of atherosclerosis.

[0076] II. Mitigation of a Symptom of Atheroscloerosis Associated withan Acute Inflammatory Response.

[0077] The atherosclerosis-inhibiting peptides of this invention arealso useful in a number of other contexts. In particular, we haveobserved that cardiovascular complications (e.g. atherosclerosis,stroke, etc.) frequently accompany or follow the onset of an acute phaseinflammatory response. Such an acute state inflammatory response isoften associated with a recurrent inflammatory disease (e.g., leprosy,tuberculosis, systemic lupus erythematosus, and rheumatoid arthritis), aviral infection (e.g. influenza), a bacterial infection, a fungalinfection, an organ transplant, a wound or other trauma, an implantedprosthesis, a biofilm, and the like.

[0078] It was a surprising discovery of this invention thatadministration of one or more of the peptide described herein, canreduce or prevent the formation of oxidized phospholipids during orfollowing an acute phase response and thereby mitigate or eliminatecardiovascular complications associated with such a condition.

[0079] Thus, for example, we have demonstrated that a consequence ofinfluenza infection is the dimunition in paraoxonase and plateletactivating acetylhydrolase activity in the HDL. Without being bound by aparticular theory, we believe that, as a result of the loss of these HDLenzymatic activities and also as a result of the association ofpro-oxidant proteins with HDL during the acute phase response, HDL is nolonger able to prevent LDL oxidation and was no longer able to preventthe LDL-induced production of monocyte chemotactic activity byendothelial cells.

[0080] We observed that in a subject injected with very low dosages ofthe polypeptides of this invention (e.g. 20 micrograms for mice) dailyafter infection with the influenza A virus paraoxonase levels did notfall and the biologically active oxidized phospholipids were notgenerated beyond background. This indicates that D-4F (and/or otherpeptides of this invention) can be administered (e.g. orally or byinjection) to patients with known coronary artery disease duringinfluenza infection or other events that can generate an acute phaseinflammatory response (e.g. due to viral infection, bacterial infection,trauma, transplant, various autoimmune conditions, etc.) and thus we canprevent by this short term treatment the increased incidence of heartattack and stroke associated with pathologies that generate suchinflammatory states.

[0081] Thus, in certain embodiments, this invention contemplatesadministering one or more of the peptides of this invention to a subjectat risk for, or incurring, an acute inflammatory response and/or at riskfor or incurring a symptom of atherosclerosis.

[0082] Thus, for example, a person having or at risk for coronarydisease may prophylactically be administered a polypeptide of thisinvention during flu season. A person (or animal) subject to a recurrentinflammatory condition, e.g. rheumatoid arthritis, various autoimmunediseases, etc., can be treated with a polypeptide of this invention tomitigate or prevent the development of atherosclerosis or stroke. Aperson (or animal) subject to trauma, e.g. acute injury, tissuetransplant, etc. can be treated with a polypeptide of this invention lomitigate the development of atherosclerosis or stroke.

[0083] In certain instances such methods will entail a diagnosis of theoccurrence or risk of an acute inflammatory response. The acuteinflammatory response typically involves alterations in metabolism andgene regulation in the liver. It is a dynamic homeostatic process thatinvolves all of the major systems of the body, in addition to theimmune, cardiovascular and central nervous system. Normally, the acutephase response lasts only a few days; however, in cases of chronic orrecurring inflammation, an aberrant continuation of some aspects of theacute phase response may contribute to the underlying tissue damage thataccompanies the disease, and may also lead to further complications, forexample cardiovascular diseases or protein deposition diseases such asamyloidosis.

[0084] An important aspect of the acute phase response is the radicallyaltered biosynthetic profile of the liver. Under normal circumstances,the liver synthesizes a characteristic range of plasma proteins atsteady state concentrations. Many of these proteins have importantfunctions and higher plasma levels of these acute phase reactants (APRs)or acute phase proteins (APPs) are required during the acute phaseresponse following an inflammatory stimulus. Although most APRs aresynthesized by hepatocytes, some are produced by other cell types,including monocytes, endothelial cells, fibroblasts and adipocytes. MostAPRs are induced between 50% and several-fold over normal levels. Incontrast, the major APRs can increase to 1000-fold over normal levels.This group includes serum amyloid A (SAA) and either C-reactive protein(CRP) in humans or its homologue in mice, serum amyloid P component(SAP). So-called negative APRs are decreased in plasma concentrationduring the acute phase response to allow an increase in the capacity ofthe liver to synthesize the induced APRs.

[0085] In certain embodiments, the acute phase response, or risktherefore is evaluated by measuring one or more APPs. Measuring suchmarkers is well known to those of skill in the art, and commercialcompanies exist that provide such measurement (e.g. AGP measured byCardiotech Services, Louisville, Ky.)

[0086] III. Mitigation of a Symptom or Condition Associated withCoronary Calcification and Osteoporosis.

[0087] We have also identified oxidized lipids as a cause of coronarycalcification and osteoporosis. Moreover, without being bound to aparticularly theory, we believe the same mechanisms are involved in thepathogenesis of calcific aortic stenosis.

[0088] Thus, in certain embodiments, this invention contemplates the useof the peptides described herein to inhibit or prevent a symptom of adisease such as polymyalgia rheumatica, polyateritis nodosa,scleroderma, idiopathic pulmonary fibrosis, chronic obstructivepulmonary disease, Alzheimers Disease, AIDS, coronary calcification,calcific aortic stenosis, osteoporosis, and the like.

[0089] IV. Preferred Peptides and their Preparation.

[0090] Preferred Peptides.

[0091] It was a discovery of this invention that class A peptides, arecapable of mitigating one or more symptoms of atherosclerosis. Class Apeptides are characterized by formation of an α-helix that produces asegregation of polar and non-polar residues thereby forming a polar anda nonpolar face with the positively charged residues residing at thepolar-nonpolar interface and the negatively charged residues residing atthe center of the polar face (see, e.g., Anantharamaiah (1986) Meth.Enzymol, 128: 626-668). It is noted that the fourth exon of apo A-I,when folded into 3.667 residues/turn produces a class A amphipathichelical structure.

[0092] One particularly preferred class A peptide, designated 18A (see,Table 1, and also Anantharamaiah (1986) Meth. Enzymol, 128: 626-668) wasmodified as described herein to produce peptides orally administrableand highly effective at inhibiting or preventing one or more symptoms ofatherosclerosis. Without being bound by a particular theory, it isbelieved that the peptides of this invention act in vivo may by pickingLup seeding molecule(s) that mitigate oxidation of LDL.

[0093] We determined that increasing the number of Phe residues on thehydrophobic face of 18A would theoretically increase lipid affinity asdetermined by the computation described by Palgunachari et al. (1996)Arteriosclerosis, Thrombosis, & Vascular Biology 16: 328-338.Theoretically, a systematic substitution of residues in the nonpolarface of 18A with Phe could yield six peptides. Peptides with anadditional 2, 3 and 4 Phe would have theoretical lipid affinity (λ)values of 13, 14 and 15 units, respectively. However, the 1 valuesjumped four units if the additional Phe were increased from 4 to 5 (to19 λ units). Increasing to 6 or 7 Phe would produce a less dramaticincrease (to 20 and 21 λ units, respectively). Therefore, we chose 5additional Phe (and hence the peptides designation as 5F). In oneparticularly preferred embodiment, the 5F peptide was blocked in thatthe amino terminal residue was acetylated and the carboxyl terminalresidue was amidated.

[0094] The new class A peptide analog, 5F inhibited, lesion developmentin atherosclerosis-susceptible mice. The new peptide analog, 5F, wascompared with mouse apo A-I (MoA-I) for efficacy in inhibitingdiet-induced atherosclerosis in these mice using peptide dosages basedon the study by Levine et al. (Levine et al. (1993) Proc. Natl. Acad.Sci. USA 90:12040-12044).

[0095] A number of other class A peptides were also produced and showedvarying, but significant degrees of efficacy in mitigating one or moresymptoms of atherosclerosis. A number of such peptides are illustratedin Table 1. TABLE 1 Preferred peptides for use in this invention.Peptide Name Amino Acid Sequence SEQ ID NO. 18A     D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F 1  2F  Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ 2  3F  Ac-D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ 3  3F14Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 4  4F  Ac-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 5  5F  Ac-D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F-NH₂ 6  6F  Ac-D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH₂ 7  7F  Ac-D-W-F-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH₂ 8Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-NH₂ 9Ac-D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F-NH₂ 10Ac-D-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F-NH₂ 11Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F-NH₂ 12Ac-D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F-NH₂ 13Ac-E-W-L-K-L-F-Y-E-K-V-L-E-K-F-K-E-A-F-NH₂ 14Ac-E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 15Ac-E-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-NH₂ 16Ac-E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F-NH₂ 17Ac-E-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F-NH₂ 18Ac-E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F-NH₂ 19Ac-E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F-NH₂ 20        AC-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ 21        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 22        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 23        Ac-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH₂ 24        Ac-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH₂ 25        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 26        Ac-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-NH₂ 27        Ac-A-F-Y-D-K-V-F-E-K-F-K-E-A-F-NH₂ 28        Ac-A-F-Y-D-K-V-F-E-K-L-K-E-F-F-NH₂ 29        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-F-F-NH₂ 30        Ac-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-NH₂ 31        Ac-L-F-Y-E-K-V-L-E-K-F-K-E-A-F-NH₂ 32        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ 33        Ac-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-NH₂ 34        Ac-A-F-Y-D-K-V-F-E-K-F-K-E-A-F-NH₂ 35        Ac-A-F-Y-D-K-V-F-E-K-L-K-E-F-F-NH₂ 36        Ac-A-F-Y-D-K-V-A-E-K-F-K-E-F-F-NH₂ 37        Ac-A-F-Y-D-K-V-F-E-K-F-K-E-F-F-NH₂ 38Ac-D-W-L-K-A-L-Y-D-K-V-A-E-K-L-K-E-A-L-NH₂ 39Ac-D-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F-NH₂ 40Ac-D-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F-NH₂ 41Ac-E-W-L-K-A-L-Y-E-K-V-A-E-K-L-K-E-A-L-NH₂ 42Ac-E-W-L-K-A-F-Y-E-K-V-A-E-K-L-K-E-A-F-NH₂ 43Ac-E-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F-NH₂ 44Ac-E-W-L-K-A-F-Y-E-K-V-F-E-K-F-K-E-F-F-NH₂ 45Ac-E-W-L-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F-NH₂ 46Ac-E-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F-NH₂ 47Ac-D-F-L-K-A-W-Y-D-K-V-A-E-K-L-K-E-A-W-NH₂ 48Ac-E-F-L-K-A-W-Y-E-K-V-A-E-K-L-K-E-A-W-NH₂ 49Ac-D-F-W-K-A-W-Y-D-K-V-A-E-K-L-K-E-W-W-NH₂ 50Ac-E-F-W-K-A-W-Y-E-K-V-A-E-K-L-K-E-W-W-NH₂ 51Ac-D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-NH₂ 52Ac-D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-NH₂ 53Ac-E-K-L-K-A-F-Y-E-K-V-F-E-W-A-K-E-A-F-NH₂ 54Ac-E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L-NH₂ 55Ac-D-W-L-K-A-F-V-D-K-F-A-E-K-F-K-E-A-Y-NH₂ 56Ac-E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L-NH₂ 57Ac-D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-NH₂ 58Ac-E-W-L-K-A-F-V-Y-E-K-V-F-K-L-K-E-F-F-NH₂ 59Ac-D-W-L-R-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ 60Ac-E-W-L-R-A-F-Y-E-K-V-A-E-K-L-K-E-A-F-NH₂ 61Ac-D-W-L-K-A-F-Y-D-R-V-A-E-K-L-K-E-A-F-NH₂ 62Ac-E-W-L-K-A-F-Y-E-R-V-A-E-K-L-K-E-A-F-NH₂ 63Ac-D-W-L-K-A-F-Y-D-K-V-A-E-R-L-K-E-A-F-NH₂ 64Ac-E-W-L-K-A-F-Y-E-K-V-A-E-R-L-K-E-A-F-NH₂ 65Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-R-E-A-F-NH₂ 66Ac-E-W-L-K-A-F-Y-E-K-V-A-E-K-L-R-E-A-F-NH₂ 67Ac-D-W-L-K-A-F-Y-D-R-V-A-E-R-L-K-E-A-F-NH₂ 68Ac-E-W-L-K-A-F-Y-E-R-V-A-E-R-L-K-E-A-F-NH₂ 69Ac-D-W-L-R-A-F-Y-D-K-V-A-E-K-L-R-E-A-F-NH₂ 70Ac-E-W-L-R-A-F-Y-E-K-V-A-E-K-L-R-E-A-F-NH₂ 71Ac-D-W-L-R-A-F-Y-D-R-V-A-E-K-L-K-E-A-F-NH₂ 72Ac-E-W-L-R-A-F-Y-E-R-V-A-E-K-L-K-E-A-F-NH₂ 73Ac-D-W-L-K-A-F-Y-D-K-V-A-E-R-L-R-E-A-F-NH₂ 74Ac-E-W-L-K-A-F-Y-E-K-V-A-E-R-L-R-E-A-F-NH₂ 75Ac-D-W-L-R-A-F-Y-D-K-V-A-E-R-L-K-E-A-F-NH₂ 76Ac-E-W-L-R-A-F-Y-E-K-V-A-E-R-L-K-E-A-F-NH₂ 77D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D- 78W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-FD-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-P-D- 79W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-FD-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D- 80W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-FD-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-P-D- 81K-L-K-A-F-Y-D-K-V-F-E-W-L-K-E-A-FD-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-P-D- 82K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-LD-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-P-D- 83W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-FD-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-P-D- 84W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-FD-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F-P-D- 85W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F

[0096] While various peptides of Table 1, are illustrated with an acetylgroup protecting the amino terminus and an amide group protecting thecarboxyl terminus, either or both of these protecting groups may beeliminated and/or substituted with another protecting group as describedherein. In particularly preferred embodiments, the peptides comprise oneor more D-form amino acids as described herein. In certain embodiments,every amino acid (e.g. every enantiomeric amino acid) of the peptides ofTable 1 is a D-form amino acid.

[0097] It is also noted that Table 1 is not fully inclusive. Using theteaching provided herein, other suitable peptides can routinely beproduced (e.g. by conservative or semi-conservative substitutions (e.g.D replaced by E), extensions, deletions, and the like). Thus, forexample, one embodiment utilizes truncations of any one or more ofpeptides identified by SEQ ID Nos: 2-20 and 39-85. Thus, for example,SEQ ID NO: 21 illustrates a peptide comprising 14 amino acids from theC-terminus of 18A comprising one or more D amino acids, while SEQ IDNOS: 22-38 illustrate other truncations. Longer peptides are alsosuitable. Such longer peptides may entirely form a class A amphipathichelix, or the class A amphipathic helix (helices) may form one or moredomains of the peptide. In addition, this invention contemplatesmultimeric versions of the peptides. Thus, for example, the peptidesillustrated in Table 1 can be coupled together (directly or through alinker (e.g. a carbon linker, or one or more amino acids) with one ormore intervening amino acids). Illustrative polymeric peptides include18A-Pro-18A and the peptides of SEQ ID NOs: 79-85 preferably comprisingone or more D amino acids, more preferably with every amino acid a Damino acid as described herein and/or having one or both terminiprotected.

[0098] It was a surprising discovery of this invention that, when theclass A peptides (e.g. as illustrated in Table 1) incorporated D aminoacids they retained their activity and, but could be administeredorally. Moreover this oral administration resulted in relativelyefficient uptake and significant serum half-life thereby providing anefficacious method of mitigating one or more symptoms ofatherosclerosis.

[0099] Using the teaching provided herein, one of skill can routinelymodify the illustrated class A peptides to produce other suitable classA peptides of this invention. For example, routine conservative orsemi-conservative substitutions (e.g. E for D) can be made of theexisting amino acids. The effect of various substitutions on lipidaffinity of the resulting peptide can be predicted using thecomputational method described by Palgunachari et al. (1996)Arteriosclerosis, Thrombosis & Vascular Biology 16: 328 338. Thepeptides can be lengthened or shortened as long as the class A α-helixstructure is preserved. In addition, substitutions can be made to renderthe resulting peptide more similar to peptide(s) endogenously producedby the subject species.

[0100] In certain embodiments, the peptides of this invention comprise“D” forms of the peptides described in U.S. Pat. No. 4,643,988, morepreferably “D” forms having one or both termini coupled to protectinggroups. Such peptides include peptides having the formulaA₁-B₁-B₂-C₁-D-B₃-B₄-A₂-C₂-B₅-B₆-A₃-C₃-B₇-C₄-A₄-B₈-B₉ (SEQ ID NO: 86)wherein A₁, A₂, A₃ and A₄ are independently aspartic acid or glutamicacid, or homologues or analogues thereof; B₁, B₂, B₃, B₄, B₅, B₆, B₇, B₈and B₉ are independently tryptophan, phenylalanine, alanine, leucine,tyrosine, isoleucine, valine or α-naphthylalanine, or homologues oranalogues thereof; C₁, C₂, C₃ and C₄ are independently lysine orarginine, and D is serine, threonine, alanine, glycine, histidine, orhomologues or analogues thereof; provided that, when A₁ and A₂ areaspartic acid, A₃ and A₄ are glutamic acid, B₂ and B₉ are leucine, B₃and B₇ are phenylalanine, B₄ is tyrosine, B₅ is valine, B₆, B₈, and Dare alanine, and C₁, C₂, C₃ and C₄ are lysine, B₁ is not Tryptophan,where at one enantiomeric amino acid is a “D” form amino acids.Preferably at least 50% of the enantiomeric amino acids are “D” form,more preferably at least 80% of the enantiomeric amino acids are “D”form, and most preferably at least 90% or even all of the enantiomericamino acids are “D” form amino acids.

[0101] While, in preferred embodiments, the peptides of this inventionutilize naturally-occurring amino acids or D forms of naturallyoccurring amino acids, substitutions with non-naturally occurring aminoacids (e.g., methionine sulfoxide, methionine methylsulfonium,norleucine, episilon-aminocaproic acid, 4-aminobutanoic acid,tetrahydroisoquinoline-3-carboxylic acid, 8-aminocaprylic acid,4-aminobutyric acid, Lys(N(epsilon)-trifluoroacetyl), α-aminoisobutyricacid, and the like) are also contemplated.

[0102] In addition to the class A peptides described herein,peptidomimetics are also contemplated herein. Peptide analogs arecommonly used in the pharmaceutical industry as non-peptide drugs withproperties analogous to those of the template peptide. These types ofnon-peptide compound are termed “peptide mimetics” or “peptidomimetics”(Fauchere (1986) Adv. Drug Res. 15: 29; Veber and Freidinger (1985) TINSp.392; and Evans et al. (1987) J. Med. Chem. 30: 1229) and are usuallydeveloped with the aid of computerized molecular modeling. Peptidemimetics that are structurally similar to therapeutically usefulpeptides may be used to produce an equivalent therapeutic orprophylactic effect.

[0103] Generally, peptidomimetics are structurally similar to a paradigmpolypeptide (i.e., 5F described herein), but have one or more peptidelinkages optionally replaced by a linkage selected from the groupconsisting of: —CH₂NH—, —CH₂S—, —CH₂—CH₂—, —CH═CH— (cis and trans),—COCH₂—, —CH(OH)CH₂—, —CH₂SO—, etc. by methods known in the art andfurther described in the following references: Spatola (1983) p. 267 inChemistry and Biochemistry of Amino Acids, Peptides, and Proteins, B.Weinstein, eds., Marcel Dekker, New York,; Spatola (1983) Vega Data 1(3)Peptide Backbone Modifications. (general review); Morley (1980) TrendsPharm Sci pp. 463-468 (general review); Hudson et al. (1979) Int J PeptProt Res 14:177-185 (—CH₂NH—, CH₂CH₂—); Spatola et al. (1986) Life Sci38:1243-1249 (—CH₂—S); Hann, (1982) J Chem Soc Perkin Trans I 307-314(—CH—CH—, cis and trans); Almquist et al. (1980) J Med Chem.23:1392-1398 (—COCH₂—); Jennings-White et al. (1982) Tetrahedron Lett.23:2533 (—COCH₂—); Szelke, M. et al., European Appln. EP 45665 (1982)CA: 97:39405 (1982) (—CH(OH)CH2—); Holladay et al. (1983) TetrahedronLett 24:4401-4404 (—C(OH)CH₂—); and Hruby (1982) Life Sci., 31:189-199(—CH₂—S—)).

[0104] A particularly preferred non-peptide linkage is —CH₂NH—. Suchpeptide mimetics may have significant advantages over polypeptideembodiments, including, for example: more economical production, greaterchemical stability, enhanced pharmacological properties (half-life,absorption, potency, efficacy, etc.), reduced antigenicity, and others.

[0105] In addition, circularly permutations of the peptides describedherein or constrained peptides (including cyclized peptides) comprisinga consensus sequence or a substantially identical consensus sequencevariation may be generated by methods known in the art (Rizo andGierasch (1992) Ann. Rev. Biochem. 61: 387); for example, by addinginternal cysteine residues capable of forming intramolecular disulfidebridges which cyclize the peptide.

[0106] Peptide Preparation.

[0107] The peptides used in this invention are chemically synthesizedusing standard chemical peptide synthesis techniques or, particularlywhere the peptide does not comprise “D” amino acid residues, arerecombinantly expressed. Where the polypeptides are recombinantitlyexpressed, a host organism (e.g. bacteria, plant, fungal cells, etc.) incultured in an environment where one or more of the amino acids isprovided to the organism exclusively in a D form. Recombinantlyexpressed peptides in such a system then incorporate those D aminoacids.

[0108] In preferred embodiments the peptides are chemically synthesizedby any of a number of fluid or solid phase peptide synthesis techniquesknown to those of skill in the art. Solid phase synthesis in which theC-terminal amino acid of the sequence is attached to an insolublesupport followed by sequential addition of the remaining amino acids inthe sequence is a preferred method for the chemical synthesis of thepolypeptides of this invention. Techniques for solid phase synthesis arewell known to those of skill in the art and are described, for example,by Barany and Merrifield (1963) Solid-Phase Peptide Synthesis; pp. 3-284in The Peptides: Analysis, Synthesis, Biology. Vol. 2: Special Methodsin Peptide Synthesis, Part A.; Merrifield et al. (1963) J. Am. Chem.Soc. 85: 2149-2156, and Stewart et al. (1984) Solid Phase PeptideSynthesis, 2nd ed. Pierce Chem. Co., Rockford, Ill.

[0109] In a most preferred embodiment, the peptides are synthesized bythe solid phase peptide synthesis procedure using a benzhyderylamineresin (Beckman Bioproducts, 0.59 mmol of NH₂/g of resin) as the solidsupport. The COOH terminal amino acid (e.g., t-butylcarbonyl-Phe) isattached to the solid support through a 4-(oxymethyl)phenacetyl group.This is a more stable linkage than the conventional benzyl esterlinkage, yet the finished peptide can still be cleaved by hydrogenation.Transfer hydrogenation using formic acid as the hydrogen donor is usedfor this purpose. Detailed protocols used for peptide synthesis andanalysis of synthesized peptides are describe in a miniprint supplementaccompanying Anantharamaiah et al. (1985) J. Biol. Chem., 260(16):10248-10255.

[0110] It is noted that in the chemical synthesis of peptides,particularly peptides comprising D amino acids, the synthesis usuallyproduces a number of truncated peptides in addition to the desiredfull-length product. The purification process (e.g. HPLC) typicallyresults in the loss of a significant amount of the full-length product.

[0111] It was a discovery of this invention that, in the synthesis of aD peptide (e.g. D-4), in order to prevent loss in purifying the longestform one can dialyze and use the mixture and thereby eliminate the lastHPLC purification. Such a mixture loses about 50% of the potency of thehighly purified product (e.g. per wt of protein product), but themixture contains about 6 times more peptide and thus greater totalactivity.

[0112] D-Form Amino Acids.

[0113] D-amino acids are incorporated at one or more positions in thepeptide simply by using a D-form derivatized amino acid residue in thechemical synthesis. D-form residues for solid phase peptide synthesisare commercially available from a number of suppliers (see, e.g.,Advanced Chem Tech, Louisville; Nova Biochem, San Diego; Sigma, StLouis; Bachem California Inc., Torrance, etc.). The D-form amino acidscan be incorporated at any position in the peptide as desired. Thus, forexample, in one embodiment, the peptide can comprise a single D-aminoacid, while in other embodiments, the peptide comprises at least two,generally at least three, more generally at least four, most generallyat least five, preferably at least six, more preferably at least sevenand most preferably at least eight D amino acids. In particularlypreferred embodiments, essentially every other (enantiomeric) amino acidis a D-form amino acid. In certain embodiments at least 90%, preferablyat least 90%, more preferably at least 95% of the enantiomeric aminoacids are D-form amino acids. In one particularly preferred embodiment,essentially every enantiomeric amino acid is a D-form amino acid.

[0114] Protecting Groups.

[0115] In certain embodiments, the one or more R-groups on theconstituent amino acids and/or the terminal amino acids are blocked witha protecting group. Without being bound by a particular theory, it was adiscovery of this invention that blockage, particularly of the aminoand/or carboxyl termini of the subject peptides of this inventiongreatly improves oral delivery and significantly increases serumhalf-life.

[0116] A wide number of protecting groups are suitable for this purpose.Such groups include, but are not limited to acetyl, amide, and alkylgroups with acetyl and alkyl groups being particularly preferred forN-terminal protection and amide groups being preferred for carboxylterminal protection. In certain particularly preferred embodiments, theprotecting groups include, but are not limited to alkyl chains as infatty acids, propeonyl, formyl, and others. Particularly preferredcarboxyl protecting groups include amides, esters, and ether-formingprotecting groups. In one preferred embodiment, an acetyl group is usedto protect the amino terminus and an amide group is used to protect thecarboxyl terminus. These blocking groups enhance the helix-formingtendencies of the peptides. Certain particularly preferred blockinggroups include alkyl groups of various lengths, e.g. groups having theformula: CH₃—(CH₂)_(n)—CO— where n ranges from about 1 to about 20,preferably from about 1 to about 16 or 18, more preferably from about 3to about 13, and most preferably from about 3 to about 10.

[0117] In certain particularly preferred embodiments, the protectinggroups include, but are not limited to alkyl chains as in fatty acids,propeonyl, formyl, and others. Particularly preferred carboxylprotecting groups include amides, esters, and ether-forming protectinggroups. In one preferred embodiment, an acetyl group is used to protectthe amino terminus and an amide group is used to protect the carboxylterminus. These blocking groups enhance the helix-forming tendencies ofthe peptides. Certain particularly preferred blocking groups includealkyl groups of various lengths, e.g. groups having the formula:CH₃—(CH₂)_(n)—CO— where n ranges from about 3 to about 20, preferablyfrom about 3 to about 16, more preferably from about 3 to about 13, andmost preferably from about 3 to about 10.

[0118] Other protecting groups include, but are not limited to Fmoc,t-butoxycarbonyl (t-BOC), 9-fluoreneacetyl group, 1-fluorenecarboxylicgroup, 9-florenecarboxylic group, 9-fluorenone-1-carboxylic group,henzyloxycarbonyl, Xanthyl (Xan), Trityl (Trt), 4-methyltrityl (Mtt),4-methoxytrityl (Mmt), 4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr),Mesitylene-2-sulphonyl (Mts), 4,4-dimethoxybenzhydryl (Mbh), Tosyl(Tos), 2,2,5,7,8-pentamethyl chroman-6-sulphonyl (Pmc), 4-methylbenzyl(MeBzl), 4-methoxybenzyl (MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl),Benzoyl (Bz), 3-nitro 2-pyridinesulphenyl (Npys),1-(4,4-dimentyl-2,6-diaxocyclohcxylidene)ethyl (Dde), 2,6-dichlorobenzyl(2,6-DiCl-Bzl), 2-chlorobenzyloxycarbonyl (2-Cl-Z),2-bromobenzyloxycarbonyl (2-Br-Z), Benzyloxymethyl (Bom), cyclohexyloxy(cHxO), t-butoxymethyl (Bum), t-butoxy (tBuO), t-Butyl (tBu), Acetyl(Ac), and Trifluoroacetyl (TFA).

[0119] Protecting/blocking groups are well known to those of skill asare methods of coupling such groups to the appropriate residue(s)comprising the peptides of this invention (see, e.g. Greene et al.,(1991) Protective Groups in Organic Synthesis, 2nd ed., John Wiley &Sons, Inc. Somerset, N.J.). In one preferred embodiment, for example,acetylation is accomplished during the synthesis when the peptide is onthe resin using acetic anhydride. Amide protection can be achieved bythe selection of a proper resin for the synthesis. During the synthesisof the peptides described herein in the examples, rink amide resin wasused. After the completion of the synthesis, the semipermanentprotecting groups on acidic bifunctional amino acids such as Asp and Gluand basic amino acid Lys, hydroxyl of Tyr are all simultaneouslyremoved. The peptides released from such a resin using acidic treatmentcomes out with the n-terminal protected as acetyl and the carboxylprotected as NH₂ and with the simultaneous removal of all of the otherprotecting groups.

[0120] V. Enhancing Peptide Uptake.

[0121] It was also a surprising discovery of this invention that when anall L amino acid peptide (e.g. otherwise having the sequence of thepeptides of this invention) is administered in conjunction with theD-form (i.e. a peptide of this invention) the uptake of the D-formpeptide is increased. Thus, in certain embodiments, this inventioncontemplates the use of combinations of D-form and L-form peptides inthe methods of this invention. The D-form peptide and the L-form peptidecan have different amino acid sequences, however, in preferredembodiments, they both have amino acid sequences of peptides describedherein, and in still more preferred embodiments, they have the sameamino acid sequence.

[0122] It was also a discovery of this invention that concatamers of theclass A amphipathic helix peptides of this invention are also effectivein mitigating one or more symptoms of atherosclerosis. The monomerscomprising the concatamers can be coupled directly together or joined bya linker. In certain embodiments, the linker is an amino acid linker(e.g. a proline), or a peptide linker (e.g. Gly₄Ser₃). In certainembodiments, the concatamer is a 2 mer, more preferably a 3 mer, stillmore preferably a 4 mer, and most preferably 5 mer, 8 mer or 10 mer.

[0123] VI. Pharmaceutical Formulations.

[0124] In order to carry out the methods of the invention, one or morepeptides or peptide mimetics of this invention are administered, e.g. toan individual diagnosed as having one or more symptoms ofatherosclerosis, or as being at risk for atherosclerosis. The peptidesor peptide mimetics can be administered in the “native” form or, ifdesired, in the form of salts, esters, amides, prodrugs, derivatives,and the like, provided the salt, ester, amide, prodrugs or derivative issuitable pharmacologically, i.e., effective in the present method.Salts, esters, amides, prodrugs and other derivatives of the activeagents may be prepared using standard procedures known to those skilledin the art of synthetic organic chemistry and described, for example, byMarch (1992) Advanced Organic Chemistry; Reactions, Mechanisms andStructure, 4th Ed. N.Y. Wiley-Interscience.

[0125] For example, acid addition salts are prepared from the free baseusing conventional methodology, that typically involves reaction with asuitable acid. Generally, the base form of the drug is dissolved in apolar organic solvent such as methanol or ethanol and the acid is addedthereto. The resulting salt either precipitates or may be brought out ofsolution by addition of a less polar solvent. Suitable acids forpreparing acid addition salts include both organic acids, e.g., aceticacid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, malicacid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaricacid, citric acid, benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid,salicylic acid, and the like, as well as inorganic acids, e.g.,hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid,phosphoric acid, and the like. An acid addition salt may be reconvertedto the free base by treatment with a suitable base. Particularlypreferred acid addition salts of the active agents herein are halidesalts, such as may be prepared using hydrochloric or hydrobromic acids.Conversely, preparation of basic salts of the peptides or mimetics areprepared in a similar manner using a pharmaceutically acceptable basesuch as sodium hydroxide, potassium hydroxide, ammonium hydroxide,calcium hydroxide, trimethylamine, or the like. Particularly preferredbasic salts include alkali metal salts, e.g., the sodium salt, andcopper salts.

[0126] Preparation of esters typically involves functionalization ofhydroxyl and/or carboxyl groups which may be present within themolecular structure of the drug. The esters are typicallyacyl-substituted derivatives of free alcohol groups, i.e., moieties thatare derived from carboxylic acids of the formula RCOOH where R is alky,and preferably is lower alkyl. Esters can be reconverted to the freeacids, if desired, by using conventional hydrogenolysis or hydrolysisprocedures.

[0127] Amides and prodrugs may also be prepared using techniques knownto those skilled in the art or described in the pertinent literature.For example, amides may be prepared from esters, using suitable aminereactants, or they may be prepared from an anhydride or an acid chlorideby reaction with ammonia or a lower alkyl amine. Prodrugs are typicallyprepared by covalent attachment of a moiety that results in a compoundthat is therapeutically inactive until modified by an individual'smetabolic system.

[0128] The peptides or mimetics identified herein are useful forparenteral, topical, oral, nasal (or otherwise inhaled), rectal, orlocal administration, such as by aerosol or transdermally, forprophylactic and/or therapeutic treatment of atherosclerosis and/orsymptoms thereof. The pharmaceutical compositions can be administered ina variety of unit dosage forms depending upon the method ofadministration. Suitable unit dosage forms, include, but are not limitedto powders, tablets, pills, capsules, lozenges, suppositories, patches,nasal sprays, injectibles, implantable sustained-release formulations,lipid complexes, etc.

[0129] The peptides and/or peptide mimetics of this invention aretypically combined with a pharmaceutically acceptable carrier(excipient) to form a pharmacological composition. Pharmaceuticallyacceptable carriers can contain one or more physiologically acceptablecompound(s) that act, for example, to stabilize the composition or toincrease or decrease the absorption of the active agent(s).Physiologically acceptable compounds can include, for example,carbohydrates, such as glucose, sucrose, or dextrans, antioxidants, suchas ascorbic acid or glutathione, chelating agents, low molecular weightproteins, protection and uptake enhancers such as lipids, compositionsthat reduce the clearance or hydrolysis of the active agents, orexcipients or other stabilizers and/or buffers.

[0130] Other physiologically acceptable compounds include wettingagents, emulsifying agents, dispersing agents or preservatives that areparticularly useful for preventing the growth or action ofmicroorganisms. Various preservatives are well known and include, forexample, phenol and ascorbic acid. One skilled in the art wouldappreciate that the choice of pharmaceutically acceptable carrier(s),including a physiologically acceptable compound depends, for example, onthe route of administration of the active agent(s) and on the particularphysio-chemical characteristics of the active agent(s).

[0131] The excipients are preferably sterile and generally free ofundesirable matter. These compositions may be sterilized byconventional, well-known sterilization techniques.

[0132] In therapeutic applications, the compositions of this inventionare administered to a patient suffering from one or more symptoms ofatherosclerosis or at risk for atherosclerosis in an amount sufficientto cure or at least partially prevent or arrest the disease and/or itscomplications. An amount adequate to accomplish this is defined as a“therapeutically effective dose.” Amounts effective for this use willdepend upon the severity of the disease and the general state of thepatient's health. Single or multiple administrations of the compositionsmay be administered depending on the dosage and frequency as requiredand tolerated by the patient. In any event, the composition shouldprovide a sufficient quantity of the active agents of the formulationsof this invention to effectively treat (ameliorate one or more symptoms)the patient.

[0133] The concentration of peptide or mimetic can vary widely, and willbe selected primarily based on fluid volumes, viscosities, body weightand the like in accordance with the particular mode of administrationselected and the patient's needs. Concentrations, however, willtypically be selected to provide dosages ranging from about 0.1 or 1mg/kg/day to about 50 mg/kg/day and sometimes higher. Typical dosagesrange from about 3 mg/kg/day to about 3.5 mg/kg/day, preferably fromabout 3.5 mg/kg/day to about 7.2 mg/kg/day, more preferably from about7.2 mg/kg/day to about 11.0 mg/kg/day, and most preferably from about11.0 mg/kg/day to about 15.0 mg/kg/day. In certain preferredembodiments, dosages range from about 10 mg/kg/day to about 50mg/kg/day. It will be appreciated that such dosages may be varied tooptimize a therapeutic regimen in a particular subject or group ofsubjects.

[0134] In certain preferred embodiments, the peptides or peptidemimetics of this invention are administered orally (e.g. via a tablet)or as an injectable in accordance with standard methods well known tothose of skill in the art. In other preferred embodiments, the peptides,may also be delivered through the skin using conventional transdermaldrug delivery systems, i.e., transdermal “patches” wherein the activeagent(s) are typically contained within a laminated structure thatserves as a drug delivery device to be affixed to the skin. In such astructure, the drug composition is typically contained in a layer, or“reservoir,” underlying an upper backing layer. It will be appreciatedthat the term “reservoir” in this context refers to a quantity of“active ingredient(s)” that is ultimately available for delivery to thesurface of the skin. Thus, for example, the “reservoir” may include theactive ingredient(s) in an adhesive on a backing layer of the patch, orin any of a variety of different matrix formulations known to those ofskill in the art. The patch may contain a single reservoir, or it maycontain multiple reservoirs.

[0135] In one embodiment, the reservoir comprises a polymeric matrix ofa pharmaceutically acceptable contact adhesive material that serves toaffix the system to the skin during drug delivery. Examples of suitableskin contact adhesive materials include, but are not limited to,polyethylenes, polysiloxanes, polyisobutylenes, polyacrylates,polyurethanes, and the like. Alternatively, the drug-containingreservoir and skin contact adhesive are present as separate and distinctlayers, with the adhesive underlying the reservoir which, in this case,may be either a polymeric matrix as described above, or it may be aliquid or hydrogel reservoir, or may take some other form. The backinglayer in these laminates, which serves as the upper surface of thedevice, preferably functions as a primary structural element of the“patch” and provides the device with much of its flexibility. Thematerial selected for the backing layer is preferably substantiallyimpermeable to the active agent(s) and any other materials that arepresent.

[0136] Other preferred formulations for topical drug delivery include,but are not limited to, ointments and creams. Ointments are semisolidpreparations which are typically based on petrolatum or other petroleumderivatives. Creams containing the selected active agent, are typicallyviscous liquid or semisolid emulsions, often either oil-in-water orwater-in-oil. Cream bases are typically water-washable, and contain anoil phase, an emulsifier and an aqueous phase. The oil phase, alsosometimes called the “internal” phase, is generally comprised ofpetrolatum and a fatty alcohol such as cetyl or stearyl alcohol; theaqueous phase usually, although not necessarily, exceeds the oil phasein volume, and generally contains a humectant. The emulsifier in a creamformulation is generally a nonionic, anionic, cationic or amphotericsurfactant. The specific ointment or cream base to be used, as will beappreciated by those skilled in the art, is one that will provide foroptimum drug delivery. As with other carriers or vehicles, an ointmentbase should be inert, stable, nonirritating and nonsensitizing.

[0137] Unlike typical peptide formulations, the peptides of thisinvention comprising D-form amino acids can be administered, evenorally, without protection against proteolysis by stomach acid, etc.Nevertheless, in certain embodiments, peptide delivery can be enhancedby the use of protective excipients. This is typically accomplishedeither by complexing the polypeptide with a composition to render itresistant to acidic and enzymatic hydrolysis or by packaging thepolypeptide in an appropriately resistant carrier such as a liposome.Means of protecting polypeptides for oral delivery are well known in theart (see, e.g., U.S. Pat. No. 5,391,377 describing lipid compositionsfor oral delivery of therapeutic agents).

[0138] Elevated serum half-life can be maintained by the use ofsustained-release protein “packaging” systems. Such sustained releasesystems are well known to those of skill in the art. In one preferredembodiment, the ProLease biodegradable microsphere delivery system forproteins and peptides (Tracy (1998) Biotechnol. Prog. 14: 108; Johnsonet al. (1996), Nature Med. 2: 795; Herbert et al. (1998), Pharmaceut.Res. 15, 357) a dry powder composed of biodegradable polymericmicrospheres containing the protein in a polymer matrix that can becompounded as a dry formulation with or without other agents.

[0139] The ProLease microsphere fabrication process was specificallydesigned to achieve a high protein encapsulation efficiency whilemaintaining protein integrity. The process consists of (i) preparationof freeze-dried protein particles from bulk protein by sprayfreeze-drying the drug solution with stabilizing excipients, (ii)preparation of a drug-polymer suspension followed by sonication orhomogenization to reduce the drug particle size, (iii) production offrozen drug-polymer microspheres by atomization into liquid nitrogen,(iv) extraction of the polymer solvent with ethanol, and (v) filtrationand vacuum drying to produce the final dry-powder product. The resultingpowder contains the solid form of the protein, which is homogeneouslyand rigidly dispersed within porous polymer particles. The polymer mostcommonly used in the process, poly(lactide-co-glycolide) (PLG), is bothbiocompatible and biodegradable.

[0140] Encapsulation can be achieved at low temperatures (e.g., −40°C.). During encapsulation, the protein is maintained in the solid statein the absence of water, thus minimizing water-induced conformationalmobility of the protein, preventing protein degradation reactions thatinclude water as a reactant, and avoiding organic-aqueous interfaceswhere proteins may undergo denaturation. A preferred process usessolvents in which most proteins are insoluble, thus yielding highencapsulation efficiencies (e.g., greater than 95%).

[0141] In another embodiment, one or more components of the solution canbe provided as a “concentrate”, e.g., in a storage container (e.g., in apremeasured volume) ready for dilution, or in a soluble capsule readyfor addition to a volume of water.

[0142] The foregoing formulations and administration methods areintended to be illustrative and not limiting. It will be appreciatedthat, using the teaching provided herein, other suitable formulationsand modes of administration can be readily devised.

[0143] VII. Lipid-Based Formulations.

[0144] In certain embodiments, the peptides of this invention areadministered in conjunction with one or more lipids. The lipids can beformulated as an excipient to protect and/or enhance transport/uptake ofthe peptides or they can be administered separately.

[0145] Without being bound by a particular theory, it was discovered ofthis invention that administration (e.g. oral administration) of certainphospholipids can significantly increase HDL/LDL ratios. In addition, itis believed that certain medium-length phospholipids are transported bya process different than that involved in general lipid transport. Thus,co-administration of certain medium-length phospholipids with thepeptides of this invention confer a number of advantages: They protectthe phospholipids from digestion or hydrolysis, they improve peptideuptake, and they improve HDL/LDL ratios.

[0146] The lipids can be formed into liposomes that encapsulate thepolypeptides of this invention and/or they can be simplycomplexed/admixed with the polypeptides. Methods of making liposomes andencapsulating reagents are well known to those of skill in the art (see,e.g., Martin and Papahadjopoulos (1982) J. Biol. Chem., 257: 286-288;Papahadjopoulos et al. (1991) Proc. Natl. Acad. Sci. USA, 88:11460-11464: Huang et al. (1992) Cancer Res., 52:6774-6781; Lasic et al.(1992) FEBS Lett., 312: 255-258., and the like).

[0147] Preferred phospholipids for use in these methods have fatty acidsranging from about 4 carbons to about 24 carbons in the sn-1 and sn-2positions. In certain preferred embodiments, the fatty acids aresaturated. In other preferred embodiments, the fatty acids can beunsaturated. Various preferred fatty acids are illustrated in Table 2.TABLE 2 Preferred fatty acids in the sn-1 and/or sn-2 position of thepreferred phospholipids for administration of D polypeptides. Carbon No.Common Name IUPAC Name  3:0 Propionoyl Trianoic  4:0 Butanoyl Tetranoic 5:0 Pentanoyl Pentanoic  6:0 Caproyl Hexanoic  7:0 Heptanoyl Heptanoic 8:0 Capryloyl Octanoic  9:0 Nonanoyl Nonanoic 10:0 Capryl Decanoic 11:0Undcanoyl Undecanoic 12:0 Lauroyl Dodecanoic 13:0 TridecanoylTridecanoic 14:0 Myristoyl Tetradecanoic 15:0 PentadecanoylPentadecanoic 16:0 Palmitoyl Hexadecanoic 17:0 HeptadecanoylHeptadecanoic 18:0 Stearoyl Octadecanoic 19:0 Nonadecanoyl Nonadecanoic20:0 Arachidoyl Eicosanoic 21:0 Heniccosanoyl Heniecosanoic 22:0Behenoyl Docosanoic 23:0 Trucisanoyl Trocosanoic 24:0 LignoceroylTetracosanoic 14:1 Myristolcoyl (9-cis) 14:1 Myristelaidoyl (9-trans)16:1 Palmitoleoyl (9-cis) 16:1 Palmitelaidoyl (9-trans)

[0148] The fatty acids in these positions can be the same or different.Particularly preferred phospholipids have phosphorylcholine at the sn-3position.

[0149] VIII. Additional Pharmacologically Active Agents.

[0150] Additional pharmacologically active agents may be delivered alongwith the primary active agents, e.g., the peptides of this invention. Inone embodiment, such agents include, but are not limited to agents thatreduce the risk of atherosclerotic events and/or complications thereof.Such agents include, but are not limited to beta blockers, beta blockersand thiazide diuretic combinations, statins, aspirin, ace inhibitors,ace receptor inhibitors (ARBs), and the like.

[0151] Suitable beta blockers include, but are not limited tocardioselective (selective beta 1 blockers), e.g., acebutolol(Sectral™), atenolol (Tenormin™), betaxolol (Kerlone™), bisoprolol(Zebeta™), metoprolol (Lopressor™), and the like. Suitable non-selectiveblockers (block beta 1 and beta 2 equally) include, but are not limitedto carteolol (Cartrol™), nadolol (Corgard™), penbutolol (Levatol™),pindolol (Visken™), propranolol (Inderal™), timolol (Blockadren™),labetalol (Normodyne™, Trandate™), and the like.

[0152] Suitable beta blocker thiazide diuretic combinations include, butare not limited to Lopressor HCT, ZIAC, Tenoretic, Corzide, Timolide,Inderal LA 40/25, Inderide, Normozide, and the like.

[0153] Suitable statins include, but are not limited to pravastatin(Pravachol/Bristol-Myers Squibb), simvastatin (Zocor/Merck), lovastatin(Mevacor/Merck), and the like.

[0154] Suitable ace inhibitors include, but are not limited to captopril(e.g. Capoten™ by Squibb), benazepril (e.g., Lotensin™ by Novartis),enalapril (e.g., Vasotec™ by Merck), fosinopril (e.g., Monopril™ byBristol-Myers), lisinopril (e.g. Prinivil™ by Merck or Zestril™ byAstra-Zeneca), quinapril (e.g. Accupril™ by Parke-Davis), ramipril(e.g., Altace™ by Hoechst Marion Roussel, King Pharmaceuticals),imidapril, perindopril erbumine (e.g., Aceon™ by Rhone-Polene Rorer),trandolapril (e.g., Mavik™ by Knoll Pharmaceutical), and the like.Suitable ARBS (Ace Receptor Blockers) include but are not limited tolosartan (e.g. Cozaar™ by Merck), irbesartan (e.g., Avapro™ by Sanofi),candesartan (e.g., Atacand™ by Astra Merck), valsartan (e.g., Diovan™ byNovartis),and in the like.

[0155] IX. Kits for the Amelioration of One or More Symptoms ofAtherosclerosis.

[0156] In another embodiment this invention provides kits foramelioration of one or more symptoms of atherosclerosis or for theprophylactic treatment of a subject (human or animal) at risk foratherosclerosis. The kits preferably comprise a container containing oneor more of the peptides or peptide mimetics of this invention. Thepeptide or peptide mimetic may be provided in a unit dosage formulation(e.g. suppository, tablet, caplet, patch, etc.) and/or may be optionallycombined with one or more pharmaceutically acceptable excipients.

[0157] The kit can, optionally, further comprise one or more otheragents used in the treatment of heart disease and/or atherosclerosis.Such agents include, but are not limited to, beta blockers,vasodilators, aspirin, statins, ace inhibitors or ace receptorinhibitors (ARBs) and the like, e.g. as described above.

[0158] In addition, the kits optionally include labeling and/orinstructional materials providing directions (i.e., protocols) for thepractice of the methods or use of the “therapeutics” or “prophylactics”of this invention. Preferred instructional materials describe the use ofone or more polypeptides of this invention to mitigate one or moresymptoms of atherosclerosis and/or to prevent the onset or increase ofone or more of such symptoms in an individual at risk foratherosclerosis. The instructional materials may also, optionally, teachpreferred dosages/therapeutic regiment, counter indications and thelike.

[0159] While the instructional materials typically comprise written orprinted materials they are not limited to such. Any medium capable ofstoring such instructions and communicating them to an end user iscontemplated by this invention. Such media include, but are not limitedto electronic storage media (e.g., magnetic discs, tapes, cartridges,chips), optical media (e.g., CD ROM), and the like. Such media mayinclude addresses to internet sites that provide such instructionalmaterials.

EXAMPLES

[0160] The following examples are offered to illustrate, but not tolimit the claimed invention.

Example 1

[0161] Several synthetic class A peptide analogs have been shown tomimic many of the properties of human apo A-I in vitro. In this example,a new peptide (5F) with increased amphipathicity, was given byintraperitoneal injection, 20 μg/daily, for 16 weeks to C57BL/6J micefed an atherogenic diet. Mouse apo A-I (MoAI) (50 μg/daily) or phosphatebuffer saline (PBS) injections were given to other mice as controls.Total plasma cholesterol levels and lipoprotein profiles were notsignificantly different among the treated group and the control groupsexcept that the mice receiving 5F or MoAI had lower high densitylipoprotein (HDL)-cholesterol when calculated as a percent of totalcholesterol. No toxicity or production of antibodies to the injectedmaterials was observed. When LDL was taken from animals injected with 5Fand presented to human artery wall cells in vitro it produced less lipidhydrodroperoxides and less LDL-induced chemotactic activity than LDLtaken from controls. Additionally, when HDL was taken from mice injectedwith 5F and presented to human artery wall cells in vitro together withhuman LDL, there were substantially less lipid hydroperoxides formed andsubstantially less LDL-induced monocyte chemotactic activity. Micereceiving peptide 5F had significantly less aortic atheroscleroticlesion area compared to mice receiving PBS. Lesion area in micereceiving MoAI was similar to that of the PBS-injected animals. Weconclude that 5F may have potential in the prevention and treatment ofatherosclerosis.

[0162] Materials and Methods

[0163] Peptides

[0164] Peptide 5F (Ac-18A[Asp Trp Leu Lys Ala Phe Tyr Asp Lys Val PheGlu Lys Phe Lys Glu Phe Phe]-NH₂) was synthesized by solid-phase peptidesynthesis (see, e.g., Anantharamaiah and Garber (1996) Meth. Enzymol.263: 267-282; Palgunachari et al. (1996) Arteriosclerosis, Thrombosis, &Vascular Biology 16: 328-338). The purity of the synthetic peptide wasestablished by analytical HPLC and ion-spray mass spectrometry. Thepeptide was dialyzed against distilled water and lyophilized beforeusing.

[0165] MoAI was isolated from the plasma of C57BL/6J mice (EDTA plasmawas purchased from Harlan Bioproducts for Science, Indianapolis, Ind.).MoAI was isolated using a combination of size-exclusion andreversed-phase column chromatography. Briefly, plasma density wasadjusted to 1.21 g/ml by addition of KBr, and centrifuged at 50,000 rpmfor 24 hours at 4° C. (Ti70 rotor; Beckman, Fullerton, Calif.). The topfraction was collected, dialyzed against water to remove KBr,lyophilized, and delipidated. The pellet was dissolved in Gn:DTT:Trissolution (3 M guanidine HCl, 1 mM dithiothreitol, and 10 mM Tris;pH=8.0), then dialyzed against the same solution using 12,000 MW-cutoffdialysis tubing in order to remove much of the apo A-II and Capolipoproteins from the sample. The sample was then dialyzed againstwater and lyophilized. The pellet was dissolved in fresh Gn:DTT:Trissolution, and proteins were separated by size-exclusion columnchromatography, using an XK26/100 column (2.6×100 cm) packed withbulk-phase Superose 12 (Pharmacia Biotech, Piscataway, N.J.)equilibrated with Gn:DTT:Tris solution. The flow rate was 0.5 ml/min,and 2.5 ml fractions were collected. Fractions corresponding to the apoA-I peak were analyzed by SDS-PACE, and further purified by preparativeC-18 reverse-phase HPLC (Anantharamaiah and Garber (1996) Meth. Enzymol.263: 267-282).

[0166] Mice

[0167] All experiments were performed using female C57BL/6J mice(Jackson Laboratory, Bar Harbor, Me.). Mice were purchased at six weeksof age, and the diet studies were begun with mice at eight weeks of age.Mice weighing 20 to 22 grams were used in the turnover studies. Allanimal studies were prospectively reviewed and approved by theInstitutional Animal Care and Use Committee of the University of Alabamaat Birmingham.

[0168] Kinetic Studies

[0169] The 5F peptide, MoAI, and human apo A-I were labeled with ¹²⁵I bythe method of Bilheimer et al. (1972) Biochim. Biophys. Acta 260:212-221. Mice were placed on a modified Thomas-Hartroft atherogenic diet(#TD88051; Teklad, Madison, Wis.) for four weeks at which time dailyintraperitoneal injections of peptide or protein dissolved in 200 μlphosphate-buffered saline (PBS) were begun. Animals injected with MoAIor human apo A-I received 50 μg per animal; those injected with 5Freceived 20 μg. Animals were not fasted for the kinetic studies andblood samples were taken under xylazine:ketamine anesthesia from theretro-orbital sinus at 15, 30, and 45 minutes, and 1, 1.5, 2, 3, 4, 6,8, 12, and 24 hours following injection. Each animal provided threeblood samples at different time points (all retro-orbital andalternating eyes), and at least three samples were collected (fromdifferent animals) at each time point. Samples were collected intoheparinized capillary tubes, then placed in microcentrifuge tubes; theplasma was separated by centrifugation. Duplicate 10 μl aliquots of eachsample were taken for radioactivity determination, using gamma counting(Cobra; Packard Instruments, Downers Grove, Ill.) for 10 minutes persample. Total plasma volume was calculated as 4.2% body weight. Eachsample was expressed as percent of injected CPM in total plasma. Free¹²⁵I was determined by trichloroacetic acid (TCA) precipitation (1 ml of10% TCA per 10 μl plasma sample). Fitting to the kinetic model was doneusing all data points, rather than averages at each time point(PKAnalyst, MicroMath Scientific Software, Salt Lake City, Utah).

[0170] Injection Protocol and Sample Collection for Lesion Studies

[0171] Mice were acquired at six weeks of age, and randomized intogroups of 20, except that a negative control group of 10 received notreatments and was given standard rodent chow. At eight weeks of age,the treatment groups were placed on a modified Thomas-Hartroftatherogenic diet (#TD88051; Teklad, Madison, Wis.), and injections werebegun. The diet was stored at 4° C. and was used for no longer thanthree months after the manufacture date in order to minimize lipidoxidation. Animals were injected intraperitoneally daily for 16 weeks,including weekends and holidays. Twenty mice in each group receiveddaily injections of 200 μl PBS (as positive controls), or 20 μg 5F in200 μPBS, or 50 μg MoAI in 200 μl PBS.

[0172] Lyophilized 5F peptide was prepared in vials, with each bottlecontaining sufficient peptide for one day's injection. The 5F peptidewas lyophilized in PBS, and was dissolved in autoclaved Milli-Q water(Millipore Corp., Bedford, Mass.) on the day of injection. The injectionvolume for all groups was maintained at 200 μl/mouse per day.

[0173] Blood samples were taken under anesthesia by retro-orbitalbleeding at study entry (pie-diet) and at the time of organ harvesting.At the end of the study (week 16), at the last bleeding, the heart andthe liver were excised. The hearts were kept in 0.9% saline solution forabout 1 hour to eliminate blood and to permit the heart muscle to relax.They were then fixed in phosphate-buffered 4% formaldehyde for at leastone week until sectioned. The livers were removed and weighed.

[0174] Histological Evaluation

[0175] Histological evaluations were performed according to the methodof Paigen et al. (Paigen et al. (1990) Arteriosclerosis 10: 316-323)with some modifications. Briefly, hearts were fixed for at least oneweek in the phosphate-buffered formaldehyde solution. After removing thelower ⅔ of the hearts, the remaining tissue was frozen in OCT medium(Tissue-Tek, Miles Inc., Elkhart, Ind.) and sectioned in a cryostat at−20° C. Alternate 20 μm sections were saved on slides, and observed forthe beginning of the aortic root. Sections were then collected for anadditional 600 μm, or until the aortic cross-section was rounded and thevalve cusps were no longer evident. Slides were stained with Oil Red O,and counterstained with hematoxylin. Stained lesion cross-sectionalareas were measured in consecutive slides 80 μm apart by image analysis(SigmaScan Pro, SPSS Scientific, Chicago, Ill.), and the average lesionarea was determined for each aortic sinus over the 400 μm length (fiveslides) providing the greatest mean lesion area.

[0176] Cocultures, Monocyte Isolation, Isolation of Lipoproteins,Determination of Lipid Hydroperoxides, and Monocyte Chemotactic Activity

[0177] Cocultures of human artery wall cells, monocyte isolation,isolation of lipoproteins by ultracentrifugation from the plasma ofnormal human donors or from mouse plasma by FPLC, and determination oflipid hydroperoxides and monocyte chemotactic activity were performedaccording to standard methods. All human subject participation was withinformed consent approved by the UCLA Human Subjects ProtectionCommittee. The protocol for testing mouse lipoproteins in the coculturewas also performed as follows: Briefly, LDL and HDL were isolated byFPLC from mouse plasma from mice fed the atherogenic diet and injectedwith vehicle (PBS), or with peptide 5F at 20 μg/mouse/day. Thecocultures were treated with human LDL at 200 μg/ml LDL protein, ormouse LDL at 200 μg/ml or with 200 μg/ml human LDL+human HDL at 350μg/ml of HDL protein or mouse HDL at 300 μg/ml or with mouse HDL aloneat 300 μg/ml. The cocultures were incubated with or without the aboveadditions for 8 hrs at 37° C. in the presence of 10% lipoproteindeficient serum (LPDS). The supernatants were collected and analyzed forAuerbach lipid hydroperoxide equivalents. The cocultures were thenwashed and incubated with fresh culture medium without serum or LPDS foran additional 8 hrs. The conditioned medium was collected and analyzedfor monocyte chemotactic activity.

[0178] Chemical and Analytical Methods-Column Cholesterol LipoproteinProfiles (CLiP)

[0179] Plasma cholesterol lipoprotein profiles were measured using ourrecently-developed CLiP method (Garber et al. (2000) J. Lipid Res.41:1020-1026). Briefly, 5 to 10 μl of plasma were analyzed using asingle Superose 6 (Pharmacia, Piscataway N.J.) column. Immediatelyfollowing the column, cholesterol reagent was introduced through amixing tee, and the eluent:reagent mixture entered a post-columnreaction coil. Cholesterol content of the eluent mixture wasspectrophotometrically detected at 500 nm, and data points werecollected into a computer. The resulting profiles were decomposed intocomponent peaks and analyzed for relative area using PeakFit (SPSSScience, Chicago, Ill.); absolute cholesterol values for totalcholesterol and each component peak were determined by comparison with acontrol sample of known values. In some cases fractions were collectedto determine distribution of radioactivity. The CLiP method allowedanalysis of individual mouse samples, avoiding the use of pooledsamples.

[0180] Antibody Detection

[0181] To determine whether daily injections of peptides elicited anyimmune response in mice, indirect ELISA titration (Engvall (1980) Meth.Enzymol. 70:419-439) was carried out with plasma taken from mice at thetime of organ collection (following sixteen weeks of daily injection).Plates were coated with the injected peptides or MoAI (10 μg/ml). Plateswere incubated overnight. After thorough washing with borate bufferedsaline (pH 8.2) containing 0.05% Tween 20, and blocking with buffer(0.1% gelatin and 0.1% BSA in borate buffer) for 1 h, 200 μl of thediluted mouse plasma (1:100 dilution) samples were serially diluted 1:1with borate-buffered saline. Biotinylated goat antibody to mouse IgG(0.1 μg/ml) was then added to the wells and the plates were treated withSA-HRP (Streptavidin-horse radish peroxidase) for an hour and developedwith ABTS and peroxide as substrate. The plates were incubated overnightat room temperature after every addition of antigen/antibody and washedthoroughly with borate buffered saline (pH 8.2) containing 0.05% Tween20, and blocked with buffer (0.1% gelatin and 0.1% BSA in borate buffer)for 1 h before the next addition.

[0182] Statistical Methods

[0183] Treatment groups were compared by two-tailed t-tests or one wayanalysis of variance (where the data were normally distributed), or byone way analysis of variance on ranks (SigmaStat; SPSS Science, Chicago,Ill.). Kinetics of peptide or protein turnover were analyzed by fittingto a first order one-compartment kinetic model assuming non-equal inputand output rates (PKAnalyst; MicroMath Scientific Software, Salt LakeCity, Utah).

[0184] Results

[0185] Kinetic Studies

[0186] The kinetics of the clearance of peptide 5F and human and mouseapo A-I from mouse plasma following intraperitoneal injection aresummarized in Table 3. TABLE 3 Summary of fitted data from kineticexperiments Time (h) to Max. % in Injected Material T½ (h) max. CPMplasma r² Human apo A-I 15.6 3.61 23.7 0.947 (50 μg/mouse) Mouse apo A-I15.7 1.74 13.5 0.928 (50 μg) 5F (20 μg) 6.22 2.36 14.29 0.895

[0187] Human and mouse apo A-I had greatly prolonged clearance comparedwith the 5F peptide. Human apo A-I and 5F had longer times to peakplasma levels than did mouse apo A-I, although peak levels achieved weregenerally similar (human apo A-I reached higher peak levels than did theother materials). Analysis of plasma samples by column chromatographydemonstrated that peptide 5F and apo A-I (both human and mouse)associated with plasma lipoproteins, especially with particles in theHDL-sized region (FIG. 6). The HDL:VLDL ratio of peptide radioactivity1.5 h following injection of 5F was 4.19±0.58 (n=3, p<0.05). Similarresults were found 5 h following injection of 5F (6.44±1.10, p<0.02).The injected peptide initially had less than 3% free ¹²⁵I by TCAprecipitation. However, 1.5 hours after injection, free ¹²⁵Iradioactivity in the plasma as a percent of total eluted radioactivitywas substantially greater for 5F being 26.9±9.4% and at 5 hours34.4±4.8%, reflecting the expected clearance of lipoproteins andlipoprotein-associated peptides. The rate of increase in theradioactivity due to free iodine from 1.5 to 5 hours was less than thatfrom injection to 1.5 hours, possibly suggesting considerable initialdegradation of the peptide in the peritoneal cavity.

[0188] Survival and Gross Morphology on the Chow or Atherogenic Diets

[0189] Only three mice died from unexplained causes during the course ofthe prolonged diet studies. Two of the animals had been receiving MoAI,and one was receiving 5F peptide. At the time of organ collection, nogross morphological differences were observed between the groups. Liverswere enlarged in all animals fed the atherogenic diet, but neither liverweights nor liver weight as a percent of body weight were differentbetween groups (Table 4). All animals on the atherogenic diet (includingPBS-injected animals) had lower body weights than the chow-fed controls(Table 4). TABLE 4 Body and liver weights following treatment. BodyWeight Liver Weight Liver: Body Diet & Subgroup (g) (g) (percentage)Chow 23.38 ± 0.52 0.99 ± 0.02 4.24 ± 0.04% Atherogenic PBS (n = 14)20.55 ± 0.32* 1.60 ± 0.04 7.84 ± 0.26% 5F (n = 15) 21.60 ± 0.28 1.61 ±0.04 7.46 ± 0.23% MoAI (n = 14) 21.16 ± 0.34 1.72 ± 0.04 8.15 ± 0.23%*

[0190] Antigenicity

[0191] Blood samples taken at the conclusion of the 16-week injectionperiod were tested for the presence of antibodies against the peptides.No antibodies were detected against peptide 5F or against MoAI (data notshown). Cross experiments, where the ELISA plates were coated withpeptides or protein which was not injected into the series of animals,produced results essentially identical to those in the directdetermination of the presence of antibodies (data not shown).

[0192] Lipoprotein and Apolipoprotein Characterization

[0193] Total and lipoprotein cholesterol values as determined by theCLiP method are presented in Table 3. Accuracy of total cholesterolvalues was confirmed by a manual cholesterol assay (Cholesterol 1000;Sigma, St. Louis, Mo.) (data not shown). No significant differences intotal or lipoprotein-fraction cholesterol levels were seen between thetreatment groups. However, when lipoprotein fractions were expressed asa percent of total cholesterol (Table 5), HDL-cholesterol comprised asignificantly lower percentage in the 5F and MoAI groups compared withthe PBS group. TABLE 5 Total and lipoprotein cholesterol levels (mg/dland percent of total cholesterol) after 16 weeks of chow or atherogenicdiet. VLDL IDL + LDL HDL TC Chow Diet   11.66 ± 2.34  23.68 ± 3.51 37.30 ± 2.52  72.64 ± 5.58  (16.61 ± 3.55%) (31.66 ± 3.61%) (51.73 ±1.75%) Atherogenic Diet PBS  88.36 ± 5.48  75.82 ± 7.64  24.36 ± 2.19188.54 ± 14.22  (47.26 ± 1.37%) (39.83 ± 1.34%) (12.91 ± 0.68%) 5F 100.34 ± 15.72  83.37 ± 8.15  17.92 ± 2.91 201.63 ± 25.21  (47.96 ±3.26%) (42.80 ± 2.51%)  (9.24 ± 1.18%*) MoAI  100.08 ± 9.73  87.86 ±8.34  19.50 ± 3.07 207.45 ± 16.94  (48.23 ± 2.75%) (42.44 ± 2.46%) (9.34 ± 1.19%*)

[0194] Interaction of Mouse Lipoproteins with Human Artery Wall Cells

[0195] We recently discovered that normal HDL inhibits three steps inthe formation of mildly oxidized LDL. In those studies (see, copendingapplication U.S. Ser. No. 09/541,468, filed on Mar. 31, 2000) wedemonstrated that treating human LDL in vitro with apo A-I or an apo A-Imimetic peptide (37pA) removed seeding molecules from the LDL thatincluded HPODE and HPETE. These seeding molecules were required forcocultures of human artery wall cells to be able to oxidize LDL and forthe LDL to induce the artery wall cells to produce monocyte chemotacticactivity. We also demonstrated that after injection of apo A-I into miceor infusion into humans, the LDL isolated from the mice or humanvolunteers after injection/infusion of apo A-I was resistant tooxidation by human artery wall cells and did not induce monocytechemotactic activity in the artery wall cell cocultures. FIGS. 7A and 7Bdemonstrates that HDL from the mice in the present study that were fedthe atherogenic diet and injected with PBS failed to inhibit theoxidation of human LDL (FIG. 7A) and failed to inhibit LDL-inducedmonocyte chemotactic activity (FIG. 7B) in human artery wall coculures.In contrast, HDL from mice fed the atherogenic diet and injected dailywith peptide 5F was as effective in inhibiting human LDL oxidation andpreventing LDL-induced monocyte chemotactic activity in the coculturesas was normal human HDL. FIGS. 7A and 7B also show that LDL taken frommice fed the atherogenic diet and injected daily with PBS was morereadily oxidized and more readily induced monocyte chemotactic activitythan LDL taken from mice fed the same diet but injected with 20 μg dailyof peptide 5F. No cytotoxicity was noted in the artery wall cellstreated with any of the lipoproteins (data not shown). Similar resultswere obtained in three of three separate experiments (data not shown).

[0196] Lesion Formation

[0197] Mean lesion cross-sectional areas are presented in FIG. 8. Asexpected, no lesions were observed in the group given normal mouse chow(data not shown). As previously reported (Paigen et al. (1990)Arteriosclerosis 10: 316-323), considerable variations in lesion areawere observed in all groups receiving the atherogenic diet. However, the5F-injected animals had significantly lower mean lesion area thanPBS-injected animals, whether analyzed by two tailed t-test (p<0.002) orby one-way analysis of variance on ranks (p<0.001; determined due to thenon-normal distribution of mean lesion areas). MoAI injection producedno difference in lesion area compared with PBS injection, and lesionarea was significantly greater than in 5F-injected animals, both byt-test (p<0.002) and by one way analysis of variance on ranks (p<0.001).

[0198] Discussion

[0199] We previously demonstrated that synthetic peptides that weredesigned to mimic the class A amphipathic helical motif were able toassociate with phospholipids, and exhibited many biological propertiessimilar to human apo A-I (3, 8, 10, 14, 15, 20). We also have shown thatwhen these peptides are administered intravenously in animals, they arefound to be associated with plasma lipoproteins (11). This study wasdesigned to address the hypothesis that a new peptide, 5F, withincreased theoretical lipid affinity, would possess anti-atherogenicproperties.

[0200] The studies presented here demonstrated that this peptide 5Fentered the plasma after interperitoneal injection and achieved plasmalevels that were roughly comparable to MoAI, but less than human apo A-I(Table 3 and FIG. 6). The plasma clearance half-time of 5F was shorterthan either mouse or human apo A-I after peritoneal injection. Afterinjection the majority of 5F was found in the region of HDL (FIG. 6),despite the fact that the preponderance of circulating cholesterol wasin the VLDL-, IDL-, and LDL-sized regions on the atherogenic diet.

[0201] Plasma cholesterol levels and distributions were notsignificantly different among the injected groups on the atherogenicdiet (Table 5). However, when the lipoprotein fractions were expressedas a percent of total cholesterol (Table 5), HDL-cholesterol comprised asignificantly lower percentage in the 5F and MoAI groups compared withthe PBS group.

[0202] Normal HDL inhibits three steps in the formation of mildlyoxidized LDL. We demonstrated that treating human LDL in vitro with apoA-I or an apo A-I mimetic peptide removed seeding molecules from the LDLthat included HPODE and HPETE. These seeding molecules were required forcocultures of human artery wall cells to be able to oxidize LDL and forthe LDL to induce the artery wall cells to produce monocyte chemotacticactivity (see copending copending application U.S. Ser. No. 09/541,468,filed on Mar. 31, 2000). We also demonstrated that after injection ofapo A-I into mice or infusion into humans, the LDL isolated from themice or human volunteers after injection/infusion of apo A-I wasresistant to oxidation by human artery wall cells and did not inducemonocyte chemotactic activity in the artery wall cell cocultures. In thepresent studies, HDL from mice that were fed the atherogenic diet andinjected with PBS failed to inhibit the oxidation of human LDL (FIG. 7A)and failed to inhibit LDL-induced monocyte chemotactic activity (FIG.7B) in the human artery wall coculures. In stark contrast, HDL from micefed the same atherogenic diet but injected with peptide 5F was found tobe as effective in inhibiting human LDL oxidation and preventingLDL-induced monocyte chemotactic activity in the cocultures as wasnormal human HDL (FIGS. 7A and 7B). LDL taken from mice fed theatherogenic diet and injected with 5F was less readily oxidized andinduced less monocyte chemotactic activity than LDL taken from mice fedthe same diet but injected with PBS (FIGS. 7A and 7B). It is possiblethat 5F interacted with LDL in the circulation (either before or afterassociating with HDL) and removed seeding molecules necessary for LDLoxidation and LDL-induced monocyte chemotactic activity in a mannersimilar to that described in vitro for a related peptide, 37pA(copending application U.S. Ser. No. 09/541,468, filed on Mar. 31,2000).

[0203] The in vitro responses of human artery wall cells to HDL and LDLfrom mice fed the atherogenic diet and injected with peptide 5F areconsistent with the protective action of 5F in vivo. Despite, similarlevels of total cholesterol, LDL-cholesterol, IDL+VLDL-cholesterol, andlower HDL-cholesterol as a percent of total cholesterol, the animals fedthe atherogenic diet and injected with 5F had significantly lower lesionscores (FIG. 8). These results are somewhat analogous to those of Shahet al. (Shah et al. (1998) Circulation 97:780-785) who found that,despite persistence of hypercholesterolemia, apo A-I preventedprogression of atherosclerotic lesions in apo E-deficient mice.

[0204] The reason that human apo A-I has been used successfully toprevent/reduce atherosclerosis in animals (Wilson et al. (1988)Arteriosclerosis 8: 737-741; Rubin et al. (1991) Nature 353:265-267;Paszty et al. (1994) J. Clin. Invest. 94:899-903; Plump et al. (1994)Proc. Natl. Acad. Sci. USA 91:9607-9611; Shah et al. (1998) Circulation97:780-785) but injection of MoAI at a close of 50 μg daily in thesestudies did not is not clear. It has been shown that MoAI does not formprotein:lipid complexes as stable as does human apo A-I (Gong et al.(1994) Biochim. Biophys. Acta 1213:335-342). Mouse HDL has also beenshown to be more easily denatured by guanidine hydrochloride than humanHDL (Gong et al. (1994) Biochim. Biophys. Acta 1213:335-342) suggestingthat amphipathic helical peptides might displace MoAI more easily frommouse HDL than human apo A-I from human HDL. These differences may ormay not explain why MoAI did not significantly reduce lesions in thisstudy. It may also be that a higher dose of MoAI is required under theconditions that we employed. In any event, the 5F peptide was highlyeffective under these conditions and MoAI was not.

[0205] The ELISA analysis of plasma at the conclusion of the injectionprotocol indicated that antibodies were not formed against the 5Fpeptide. This was not surprising in that lipid-associating peptides havebeen shown not to produce antibodies, presumably because these peptidesbind lipids in such a way as to prevent the exposure of epitopesnecessary to elicit an immune response (Muranishi (1997) J. Pharm. Soc.Japan 117:394-404; Fricker and Drewer (1996) J Peptide Sci. 2:195-211).

[0206] A preliminary study by us suggested that transgenic miceexpressing a class A amphipathic helical peptide (37pA) withtheoretically less lipid affinity than the peptide used in this studymay have been resistant to atherosclerosis (Garber et al. (1997)Circulation 96:1-490). The current study suggests that peptide 5F likelyhas great potential for elucidating the mechanisms involved inatherogenesis and also has therapeutic potential.

Example 2 Efficacy of D Peptides

[0207] This example demonstrates the efficacy of D peptides of thisinvention. Human aortic wall cocultures were incubated with medium alone(LDL, NO CELLS or CELLS, NO LDL, see FIG. 9), control LDL from normalsubjects at 250 μg/ml (LDL) and LDL plus control HDL from normalsubjects at 350 μg/ml (+HDL). Other cocultures were incubated With thecontrol LDL together with varying amounts (micrograms shown on theabscissa) of either D-2F, or L-2F (third panel from the left, 2F) orD-37-pA or L-37pA (last panel on the right, 37pA). The data representmean±SD of values obtained from quadruplicate cocultures. Values for HDLor added peptides were all significantly different from LDL, alone(first panel on the left) at the level of p<0.01.

[0208] The cocultures were incubated for 4 hrs at 37° C. in the presenceof 10% LPDS to produce mildly oxidized LDL. The supernatants were thendiscarded, the cocultures were washed and incubated with culture mediumwithout serum or LPDS for an additional 4 hrs. This conditioned mediumwas collected and analyzed for monocyte chemotactic activity. As shownin FIG. 9, treating LDL with the D peptides in vitro prevents theiroxidation by artery wall cells.

[0209]FIG. 10 demonstrates that giving the D peptides to mice renderstheir red blood cells resistant to hemolysis (a phenomenon due tooxidation as it can be prevented with Vitamin E, data not shown). Groupsof LDL receptor deficient mice (n=3) commonly used as an animal model ofatherosclerotic lesion formation were administered the D-peptides or thesaline vehicle by gavage. Each animal was administered 100 μl of saline,100 μg/100 μl of peptide D-2F or peptide D-37pA. Blood was collectedfrom retroorbital sinus under mild anesthesia 17 n and 48 hrs later. Redcells were separated by centrifugation, were diluted to 10% hematocritwith PBS and incubated at 37° C. with gentle mixing. Aliquots wereremoved at time points t=0, 2, 6 and 18 hrs, cell pellets spun down andthe optical density due to the released hemoglobin determined.

[0210]FIG. 11 demonstrates that administering the D peptides to mice bygavage and then isolating their LDL renders the LDL resistant to arterywall cell oxidation as measured by the monocyte chemotaxis bioassay.

[0211] Another experiment demonstrated that the D-peptide was absorbedfrom the stomach and rendered LDL unable to induce monocyte chemotacticactivity in our human artery wall cell coculture model while theL-peptide of 2F did not have this property. Either saline or 2Fsynthesized from D amino acids or from L amino acids was instilled inthe stomachs of mice by gavage (instillation in the stomach by tube).After gavage the mice were bled and their LDL isolated and added to thehuman artery wall cell cocultures. The D-peptide when given by gavageprotected the LDL as evidenced by the reduced monocyte chemotaxisinduced by the LDL taken from the mice that received the D-2F peptide(D2FLDL) (synthesized from D amino acids), while the LDL taken from micethat received the L-2F (synthesized from the natural L amino acids)(L2FLDL) readily induced monocyte chemotaxis (see FIG. 12).

[0212] 2F synthesized from L amino acids when presented to LDL in vitrowas as effective as the 2F synthesized from the D amino acids (see FIG.9). Thus, the difference in the results with this experiment where thepeptides were given in vivo by gavage indicate that the 2F synthesizedfrom D amino acids must have been absorbed intact from the stomach whilethe 2F peptide synthesized from the natural L amino acids must have beendegraded in the stomach in the process of digestion and/or in the plasmaas we hypothesized would be the case. In other studies we have not seenevidence of antibody formation against the D-2F peptide.

[0213]FIG. 13A and FIG. 13B are two graphs from experiments in which LDLreceptor knockout mice were given 50 micrograms of D-5F by gavage. Theanimals were bled 1.5, 3 or 6 hours later and their HDL, LDL, andVLDL/IDL isolated. As indicated in the graph, HDL taken 1.5 hours aftergavage did not protect control (cont.) LDL from modification but the HDLtaken after 3 hours and slightly less after 6 hours following gavagewere as protective against LDL-induced monocyte chemotactic activityproduction by human artery wall cells as a control HDL (FIG. 13A). Inthe other graph (FIG. 13B), 1.5, 3, or 6 h after administration of 50micrograms of D-5F by gavage mouse LDL and VLDL/IDL were isolated. Inthe left panel a control LDL was added to the human artery wall cellswithout or with a control HDL and monocyte chemotactic activity producedby the artery wall cells was measured. In the middle panel the mouse LDLtaken after 1.5, 3, or 6 hours after gavage of 50 micrograms of D-5Fwere added to the artery wall cells. The results indicate that after 3 hand 6 h the LDL induced significantly less monocyte chemotacticactivity. On the right side of the graph the VLDL/IDL fraction oflipoproteins (V/I LDL) were added and as shown the 3 hour time pointinduced significantly less monocyte chemotactic activity.

Example 3 Effects of Increasing Hydrophobicity on the Physical-Chemicaland Biological Properties of a Class A Amphipathic Helical Peptide

[0214] List of Abbreviations

[0215] Ac₂O, acetic anhydride; apo A-I, apolipoprotein A-I; BSA, Bovineserum albumin; CAD, coronary artery disease; CD, circular dichroism;DMPC, dimyristoyl phosphatidylcholine; DiPoPE, Di (16:1) palmitoleoylphosphatidylethanomine; DSC, Differential Scanning Calorimetry; EDTA,ethylene diamine tetraacetic acid; EPC, Egg phosphatidylcholine; FMOC,Fluorinylmethyloxycarbonyl; Gdn HCl, Guanidine Hydrochloride; HAEC,human aortic endothelial cells; HASMC, human aortic smooth muscle cells;HBTU, 2-(H-benzotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate; HDL, high density lipoprotein; HPLC, HighPerformance Liquid Chromatography; LCAT, lecithin cholesterol acyltransferase; MCP-1, monocyte chemotactic protein-1; M-CSF, macrophagecolony-stimulating factor; MLV multilamellar vesicles; NMM,N-methylmorpholine; PBS, phosphate buffered saline; PIPES,piperazine-N,N′-bis[2-ethanesulfonic acid]; RP-HPLC, reverse phase highperformance liquid chromatography; TFA, trifloroacetic acid.

[0216] Abstract

[0217] We have shown that a class A amphipathic peptide 5F withincreased amphipathicity protected mice from diet-inducedatherosclerosis. We also examined the effects of increasing thehydrophobicity of a series of homologous class A amphipathic peptides,including 5F, on physical and functional properties related toatherosclerosis inhibition by systematically replacing existing nonpolaramino acids with phenylalanine. The peptides, based on the sequenceAc-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ (SEQ ID NO: 1, Ac-18A-NH₂ or2F) were: 3F³(Ac-F³18A-NH₂), 3F¹⁴(Ac-F¹⁴18A-NH₂),4F(Ac-F^(3, 14)18A-NH₂), 5F(Ac-F^(11, 14, 17)18A-NH₂),6F(Ac-F^(10, 11, 14, 17)18A-NH₂) and7F(Ac-F^(3, 10, 11, 14, 17)18A-NH₂). Measurements of aqueous solubility,HPLC retention time, exclusion pressure for penetration into an egg PCmonolayer, and rates of egg PC solubilization revealed an abruptincrease in the hydrophobicity between peptides 4F and 5F; this wasaccompanied by increased ability to associate with phospholipids. Thepeptides 6F and 7F were less effective, indicating a limit to increasedhydrophobicity for promoting lipid interaction in these peptides.Despite this marked increase in lipid affinity, these peptides were lesseffective than apoA-I in activating the plasma enzyme,lecithin:cholesterol acyl transferase (LCAT), with 5F activating LCATthe best (80% of apoA-I). Peptides 4F, 5F and 6F were equally potent ininhibiting LDL-induced monocyte chemotactic activity. These studiessuggest that an appropriate balance between peptide-peptide andpeptide-lipid interactions is required for optimal biological activityof amphipathic peptides. These studies provide a rationale for thedesign of small apoA-I-mimetics with increased potency foratherosclerosis inhibition.

[0218] Introduction.

[0219] Plasma levels of high density lipoproteins (HDL) andapolipoprotein A-I (apo A-I), the major protein constituent of HDL, areinversely correlated to coronary artery disease (CAD) (Sprecher et al.(1993) Arterioscler. Thromb. 13: 495-504; Philips et al. (1993)Circulation 88: 2762-2770). Human apo A-I is a 243 residue protein,containing eight 22-mer amphipathic helical repeats, the majority ofwhich have been shown to possess the Class A motif (Segrest et al.(1990) Proteins 8: 103-117; Anantharamaiah et al. (1993) pp. 109-142 In:The Amphipathic Helix (Epand, R. M., ed), CRC Press, Boca Raton, Fla.).Class A amphipathic helices have a characteristic charge distribution;they have a cluster of positively charged amino acids at thepolar/nonpolar boundary of the α helix and negatively charged residuesat the center of the polar face (Segrest et al. (1990) Proteins 8:103-117; Anantharamaiah et al. (1993) pp. 109-142 In: The AmphiphathicHelix (Epand, R. M., ed), CRC Press, Boca Raton, Fla.; Segrest et al.(1992) J. Lipid Res. 33: 141-166). This unique secondary structuralmotif has been postulated to be responsible for the lipid-associatingproperty of apo A-I (Segrest et al. (1990) Proteins 8: 103-117). Manystudies with synthetic analogues of Class A amphipathic helices havesupported this concept (Segrest et al. (1994) Adv. Prot. Chem. 45:303-369; Brouillette and Anantharamaiah (1995) Biochim. Biophys. Acta1256: 103-129). Recently, we have synthesized each of the putative 22mer helices present in human apo A-I as monomers and tandem dimers andshown that the N- and C-terminal amphipathic helices possess the maximumlipid-associating ability (Mishra et al. (1998) Biochemistry 37:10313-10324). X-ray crystal structure and molecular modeling studies ofthe exon 4 (44-243 residues) of apo A-I suggests that a self-associatedstate of the entire apo A-I is necessary for lipid association (Borhaniet al. (1999) Proc. Natl. Acad. Sci. USA. 94:12291-12296; Segrest et al.(2000)Current Opin. Lipidol. 11:105-115). In this model, two moleculesof apo A-I are arranged in the form of a head-to-tail dimer with themonomers interacting with each other to stabilize the lipid-associatedstructure of apo A-I.

[0220] Experimental evidence suggests that the protective effect of apoA-I and HDL against coronary artery disease could be due to their rolein “reverse cholesterol transport” (Fielding and Fielding (1995) J.Lipid Res. 36: 211-228; Glomset (1968) J. Lipid Res. 9:155-167). Reversecholesterol transport is the sum of three steps involving HDL/apo A-I,a) efflux of cholesterol from xx cells ( Johnson et al. (1991) Biochim.Biophys. Acta. 1085: 273-298; Oram and Yokoyama (1996) J. Lipid Res. 37:2473-2491), b) esterification by LCAT of HDL-associated cholesterol(Fielding et al. (1972) Biochem. Biophys. Res. Comm. 46: 1493-1498;Jonas (1991) Biochim. Biophys. Acta 1084: 205-220) and c)receptor-mediated delivery of cholesterol ester to the liver (Kreiger(1999) Ann Rev. Biochem. 68: 523-558). In vivo studies have shown thatboth human apo A-I and a class A synthetic amphipathic helical peptideinhibit atherosclerosis without altering plasma cholesterol levels by amechanism that is independent of reverse cholesterol transport (Shah etal. (1998) Circulation 97: 780-785). Recently, we have suggested thatinhibition of LDL-induced monocyte chemotaxis into artery wall cells hasbeen suggested to be another major role played by apo A-I and HDL inpreventing atherosclerosis (Navab et al. (2000) J. Lipid Res. 41:1481-1494; Navab et al. (2000) J. Lipid Res. 41: 1495-1508).

[0221] A peptide that has been shown to mimic the properties of humanapo A-I, 18A, has also been shown to possess LCAT activating(Anantharamaiah et al. (1990) Arteriosclerosis 10: 95-105; Epand et al.(1987) J. Biol. Chem. 262: 9389-9396) and cholesterol effluxingabilities (Davidson et al. (1994) J. Biol. Chem. 269: 22975-22982;Yancey et al. (1995) Biochemistry, 34: 7955-7965). Neutralizing theterminal charges of 18A to form Ac-18A-NH₂ was shown to increase itslipid affinity, and biological activities (Yancey et al. (1995)Biochemistry, 34: 7955-7965; Venkatachalapathi et al. (1993) Proteins:Structure, Function and Genetics. 15: 349-359). Several modifications ofthe amino acid sequence of this ‘parent’ molecule, 18A, have been madein an attempt to improve its apo A-I mimicking properties (Brouilletteand Anantharamaiah (1995) Biochim. Biophys. Acta 1256: 103-1291; Mishraet al. (1994) J. Biol. Chem. 269: 7185-7191; Mishra et al. (1995) J.Biol. Chem. 270:1602-1611). Our earlier studies (Brouillette andAnantharamaiah (1995) Biochim. Biophys. Acta 1256: 103-1291; Epand etal. (1987) J. Biol. Chem. 262: 9389-9396) have shown that an increase inthe hydrophobicity of this peptide increases its lipid affinity and apoA-I-mimicking properties. A synthetic peptide 5F, an analog ofAc-18A-NH₂ with increased amphipathicity has been shown to inhibitdiet-induced atherosclerosis in mice (see, e.g., Examples 1 and 2).However, the peptide 2F did not significantly inhibit diet-inducedlesion formation in C57 BL6 mice (Garber et al. (1999) Circulation 100:1538). A study of 18A dimer peptides indicated that increasedpeptide-peptide association decreased peptide:lipid association (Mishraet al. (1995) J. Biol. Chem. 270: 1602-1611). To determine the maximumextent to which the lipid affinity of the 18A peptide can be increasedwith a positive effect on lipid-associating and apo A-I-mimickingproperties, we designed a homologous series of peptides in which Pheresidues were systematically increased by substituting hydrophobic aminoacids such as, Leu and Ala on the nonpolar face with Phe. According tothe experimentally determined hydrophobicity scale of Wimley and White(Wimley and White (1996) Nature Struc. Biol. 3: 842-848), Trp and Pheare the most hydrophobic amino acids in the sense that they exhibit thegreatest partitioning into the membrane from the aqueous phase. Weelected to use Phe to increase the hydrophobicity of the peptide becauseit is the most acid-resistant hydrophobic amino acid in membrane activepeptides and Phe-containing peptides can be synthesized more easily thanTrp-containing peptides. The effects of this increase in hydrophobicityon the physical and lipid associating properties, and apo A-I-mimickingbiological properties such as LCAT activation and inhibition ofLDL-induced chemotactic activities, were studied.

[0222] Experimental Procedures.

[0223] Peptide Synthesis.

[0224] The peptides were synthesized by the solid phase method using anautomated solid phase synthesizer (PS3 Protein Technologies, Woburn,Mass.). FMOC-amino acids were coupled to a rink amide resin [0.536mEq/g], (Peninsula Laboratories, Inc. Belmont, Calif.) in the presenceof HBTU and NMM, and acetylated with acetic anhydride at the N-terminus.The peptides were cleaved from the solid support using 70% TFA indichloromethane in presence of anisolc (1%), mercaptoeathanol (0.1%) andtryptophan (20% by weight of the peptide resin) and purified on a VYDACC-4 (22 mm×25 cm, particle size 10 μm) reversed phase HPLC (RP-HPLC)column using a gradient of 25% to 58% acetonitrile in water containing0.1% TFA in 66 min. with a flow rate of 4.8 ml/min. The purity of thepeptides was verified by analytical RP-HPLC using a C₁₈ column (VYDAC,4.6 mm×25 cm, 5 μm) and a linear acetonitrile-water (in presence of 0.1%TFA) gradient of 25% to 58% in 33 min., and by the mass spectralanalysis.

[0225] Circular Dichroism.

[0226] CD spectra were recorded on an AVIV 62DS spectropolarimeter asdescribed by Mishra et al. (1994) J. Biol. Chem. 269: 7185-7191.Briefly, spectra were obtained using a cell with a 0.1 cm path lengthand measurements were taken every nm from 260 nm to 190 nm at 25° C. Allthe CD spectra were signal averaged by adding four scans, base linecorrected and smoothed. Peptide solutions in PBS, pH 7.4, were used at aconcentration of 11 μM. Peptide-DMPC complexes (1:20 mol:mol) were usedto determine the effect of lipid binding on the helicity of thesepeptides. These complexes were prepared by adding the appropriate volumeof peptide solution to DMPC multilamellar vesicles. DMPC multilamellarvesicles were prepared as follows: A known amount of lipid was dissolvedin ethanol and the solvent was removed by evaporating slowly under athin stream of nitrogen. Residual solvent was removed by storing thelipid film under vacuum overnight. An appropriate volume of PBS, pH 7.4was added to the thin lipid film to give the required finalconcentration of DMPC. The lipid-peptide complexes were prepared byadding the required volume of peptide solutions to give a lipid topeptide molar ratio of 20:1. Due to the poor solubility of thesepeptides, a peptide concentration of 11 μM was used. The mean residueellipticity, [θ]_(MRE) (deg. cm². dmol⁻¹) at 222 nm was calculated usingthe following equation:

[θ]_(MRE) =MRW[θ]/10cl

[0227] where, MRW is mean residue weight of the peptide, θ is theobserved ellipticity in degrees, c is the concentration of the peptidein g/ml, and l is the path length of the cell in centimeters. Thepercent helicity of the peptide was estimated from the followingequation as described by Morrisett et al. (1973) Biochemistry, 12:1290-1299:

% α helicity=([θ]₂₂₂+3,000)/(36,000+3,000)

[0228] where, [θ]₂₂₂ is the mean residue ellipticity at 222 nm.

[0229] Differential Scanning Calorimetry.

[0230] DSC studies were carried out using a Microcal MC-2 scanningcalorimeter (MicroCal, Inc., Amherst, Mass.) at a scan rate of 20° h⁻¹for DMPC, and 37° C. h⁻¹ for DiPoPE, using the procedure described byMishra et al. (1994) J. Biol. Chem. 269: 7185-7191. A known amount ofphospholipid was dissolved in chloroform. For one set of samples,peptide was dissolved in methanol and added to a solution of DiPoPE inchloroforn/methanol (2:1, v:v). For both, pure lipid samples and theorganic solutions of lipid and peptide, solvent was removed under a slowstream of nitrogen. Residual solvent was removed under vacuum. Buffer(PBS, pH 7.4, for DMPC or 20 mM PIPES, 1 mM EDTA, 150 mM NaCl and 0.002%NaN₃, pH 7.4, for DiPoPE) alone or a known concentration of peptidesolution in buffer to give a specific lipid/peptide molar ratio wasadded to the dried film and hydrated by vortexing at room temperaturefor 30 min. For DMPC, four consecutive scans with a 60 minuteequilibration time between scans were taken. DSC thermograms wereanalyzed using the software provided by MicroCal Inc., Amherst, Mass.,and Origin, version 5.0.

[0231] Surface Pressure Measurements.

[0232] Monolayer exclusion pressure measurements give the affinity ofthe peptides for a lipid-water interface; the procedure of Phillips andKrebs (Phillips and Krebs (1986) Methods Enzymol. 128: 387-403; Ibdah etal. (1989) Biochim. Biophys. Acta 1004: 300-308) was followed. Aninsoluble monolayer of egg phosphatidylcholine (EPC) was spread at theair-water interface in a Teflon dish at room temperature to give aninitial surface pressure (π_(i)) in the range of 5-45 dyn/cm. A solutionof peptides in PBS containing 1.5M Gdn. HCl was carefully injected in tothe subphase to give a final concentration of 50 μg/dL. The Gdn. HCl wasdiluted in the subphase to a final concentration of ≦1 mM to allow thepeptides to renature. The subphase was stirred continuously and theincrease in EPC monolayer surface pressure (Δπ) was recorded until asteady state value was obtained. The value of the initial surfacepressure (π_(i)) at which the peptides no longer penetrate the EYPCmonolayer i.e. the exclusion pressure (π_(e)), was calculated byextrapolating the π_(i) vs Δπ linear regression fit to Δπ=0 dyn/cm.

[0233] Right Angle Light Scattering Measurements.

[0234] Association of these peptides with egg phosphatidylcholine wasdetermined by following the dissolution of EPC multilamellar vesicles(MLV) by right angle light scattering using a SLM 8000C photon countingspectrofluorometer as described in (Mishra et al. (1994) J. Biol. Chem.269: 7185-719 1). EPC MLVs were prepared by evaporating a solution ofEPC (Avanti Polar, AL) under nitrogen and hydrating the lipid film withphosphate-buffered saline (pH 7.4). The sample containing 105 μM EPC andan equimolar amount of peptide was maintained at 25° C. and continuouslystirred. Turbidity clarification was monitored for 30 min. Completedissolution of EPC vesicles was achieved by addition of Triton X-100 toa final concentration of 1 mM.

[0235] Lecithin:Cholesterol Acyltransferase (LCAT) Purification.

[0236] LCAT was isolated from fresh normolipidemic plasma by the methodof Albers et al. (1986) Methods Enzymol. 129: 763-783, with somemodifications. The density of the plasma was adjusted to 1.21 g/ml andit was centrifuged at 175,000 g for 24 h. The LCAT containing fractionwas subjected to Affi-Gel Blue chromatography followed by DE-52chromatography. LCAT was eluted from the DE-52 column using a 75 to 200mM NaCl gradient in Tris buffer (10 mM, pH 7.6). SDS-PAGE showed greaterthan 90% purity of the enzyme with no human apo A-I contamination.

[0237] Assay of LCAT Activity:

[0238] The substrate was prepared by sonicating egg PC/cholesterol(90:20 mol/mol) containing trace amounts of 7α-³H cholesterol in aBranson 250 sonifier for 12 mins to obtain small unilamellar vesicles.The substrate (50 μl) was incubated with 5 μg of peptide or human apoA-I and 50 μl of BSA (40 μg/ml) for 1 h at 37° C. The total volume wasbrought up to 150 μl. After incubating for 1 h, 100 μl of LCAT was addedand incubated for 1 h at 37° C. and the reaction was quenched byspotting 10 μl on a silica strip. Cholesterol and cholesteryl ester wereseparated by thin layer chromatography of the silica strip inhexane:chloroform (2:1 v/v) mixture. Cholesterol and cholesteryl oleatestandards were visualized by immersing the TLC plate in a 3% cupricacetate, 8% phosphoric acid buffer and heating it. The positions of thestandards were used to cut the strip into two and the two parts werecounted in scintillation fluid in a Packard Tri Carb 4530. All reactionswere done in triplicate. The activation of LCAT by the peptides isexpressed as a percentage of the total activation by apo A-I .

[0239] Electrophoresis:

[0240] Non-denaturing and SDS-PAGE and was carried out using the methodof Laemmli (1970) Nature 227: 680-685. Premade Novex gels were used andthe gel was stained with Coomassie blue to identify the protein bands.

[0241] LDL-Induced Monocyte Chemotactic Activity LDL-Induced MonocyteChemotactic Activity:

[0242] Cocultures of human artery wall cells, monocyte isolation,isolation of lipoproteins by ultracentrifugation from the plasma ofnormal human donors or from mouse plasma by FPLC, and determination oflipid hydroperoxides and monocyte chemotactic activity were performed asas described by Navab et al. (Navab et al. (1991) J. Clin. Invest. 88:2039-2046; Navab et al. (1977) J. Clin. Invest. 99: 2005-2019). Briefly,LDL and HDL were isolated from human plasma by the method of Havel etal. (Havel et al. (1955) J. Clin. Invest. 43:1345-1353). Human aorticendothelial cells (HAEC) and smooth muscle cells (HASMC) were isolatedas described by Navab et al. (1991) J. Clin. Invest. 88: 2039-2046.Microtitre plates were treated with 0.1% gelatin at 37° C. overnight.HASMC were added at a confluent density of 1×10⁵ cells/cm². Cells werecultured for two days, at which time they had covered the entire surfaceof the well and had produced a substantial amount of extracellularmatrix. HAEC were subsequently added at 2×10⁵ cells/cm² and were allowedto grow, forming a complete monolayer of confluent HAEC in two days. Inall experiments, HAEC and autologous HASMC (from the same donor) wereused at passage levels of four to six. Monocytes were isolated bloodfrom normal donors as described by Fogelman et al. (1988) J. Lipid Res.29: 1243-1247. The cocultures were treated with native LDL(250 μgprotein/ml) or presence of HDL (350 μg protein/ml) or peptides for 8 h.The cocultures were then washed and incubated with medium 199 for anadditional 8 h. The resulting coculture supernatants were assayed formonocyte chemotactic activity as described by Navab et al. (1997) J ClinInvest, 99: 2005-2019.

[0243] Results.

[0244] Analysis of the Peptides.

[0245] Table 6 shows the sequences of the various 18A analogues thatwere synthesized. The peptide Ac-18A-NH₂, which has two Phe residues atpositions 6 and 18 (close to the interfacial Lys residues) is referredto as 2F. Two 3F peptides were synthesized, 3F³ or 3F¹⁴, where Leu inposition 3 and 14 (both present at the center of the nonpolar face) isreplaced by Phe, respectively. Peptide 4F has two Phe residues at thecenter of the nonpolar face that is a result of substitution of twocentral Leu residues. The substitutions in the peptides (3F to 7F) areshown in Table 6. With an increase in the number of Phe residues thetheoretical hydrophobicity per residue on the nonpolar face increasesfrom 2.05 for the peptide, 2F, to 3.15 for 7F. TABLE 6 Modifications ofAc-18A-NH₂ to increase hydrophobicity Theoretical lipid affinity PeptideSequence¹ Hydrophobicity² (A)³ 2F Ac-18A-NH₂ 2.05 13.03 3F³Ac-[F³18A]-NH₂ 2.20 13.84 3F¹⁴ Ac-[F¹⁴18A]-NH₂ 2.20 13.79 4FAc-[F^(3, 14)18A]-NH₂ 2.35 14.59 5F Ac-[F^(11, 14, 17)18A]-NH₂ 2.8119.07 6F Ac-[F^(10, 11, 14, 17)18A]-NH₂ 2.96 19.87 7F Ac-[F^(3, 10, 11,)3.15 20.78 14, 1718A]-NH₂

[0246] The peptides were purified on a preparative Vydac C₄ column byreversed-phase (RP)-HPLC using water (with 0.1% trifluoroacetic acid)and acetonitrile (0.1% trifluoroacetic acid). The purity and theretention times of the peptides were determined on an analytical VydacC₁₈ column using a gradient of 25%-58% acetonitrile in water containing0.1% TFA. The purity of these peptides was also confirmed by massspectrometry. The mass was in agreement with the calculated molecularweight. The retention times of the peptides are listed in Table 7.Although both the 3F peptides and 4F have additional Phe residuescompared to 2F, the retention times of these peptides on the C₁₈ columnare not very different (˜22 min). A sudden increase in the retentiontime is apparent with 5F, 6F and 7F (˜26 min). With increasing number ofPhe residues, the solubility of these peptides in PBS decreases. As canbe seen from Table 7, the solubility of 2F, 3F³, 3F¹⁴ and 4F (1.25 to1.4 mg/ml) are significantly higher than those of 5F, 6F and 7F (0.03 to0.1 mg/ml). TABLE 7 Physical Properties of the F-peptides. MonolayerMolecular Retention Solubility Exclusion Peptide Weight¹ Time (mins)²(mg/ml)³ Pressure(π_(c))⁴ apo A-I 28000 28.0 >2.0 34 18A 2200 19.8 >2.030 37pA 4580 26.0 >2.0 41 2F 2242 22.5 >2.0 38 3F³ 2276 21.0 1.25 383F¹⁴ 2276 21.2 1.45 39 4F 2310 22.0 1.30 40 5F 2429 26.5 0.10 45 6F 246227.0 0.03 46 7F 2510 26.0 0.10 45

[0247] The self-association of these amphipathic peptides was examinedby non-denaturing polyacrylamide gel electrophoresis (PAGE). FIG. 14shows the mobility of 2F on both denaturing SDS (FIG. 14A) and onnon-denaturing (FIG. 14B) gels. The molecular weight of 2F is 2242 andit can be seen as a single band on the SDS gel (FIG. 14A) movingslightly lower than the lowest molecular weight standard (3.5-2.5 kDa).However, under non-denaturing conditions it forms aggregates in aconcentration dependent manner as seen in FIG. 14B. At lowerconcentrations (100 μg/ml) it forms aggregates of two sizes while at ahigher concentration (250 μg/ml) only the bigger aggregates are observed(FIG. 14B). All the other peptides studied also exhibited aggregationunder non-denaturing conditions suggesting that the peptides possess astrong tendency to self-associate.

[0248] Circular Dichroism.

[0249] The secondary structure of the peptides was determined bycircular dichroism spectroscopy. Table 8 shows the percent helicity ofthe peptides in PBS and in the presence of DMPC. In PBS, homologues 2F,4F, 5F, 6F and 7F have a higher percentage helicity than 3F³ and 3F¹⁴(Table 8). Since 5F, 6F and 7F were sparingly soluble in PBS, the CDstudies were carried out using 11 μM of the peptides (a concentration atwhich they were all soluble). Peptide 2F showed 55% helicity, comparableto 5F in solution. Both 6F and 7F were slightly more helical (67% and58% respectively) while 4F was slightly less (45%). Both the 3F peptideswere much less (≈20%) helical. However, binding to DMPC considerablyincreased the helicity of all the peptides except for 6F (Table 8). In alipid environment, 2F, 5F and 7F showed a high helical content (68% to76%). Although, the peptides 3F³ and 3F¹⁴ had a very small helicalcontent in PBS, there was a significant increase in helicity in a lipidenvironment, from about 22% to 42% for 3F³, and from 19% to 55% for3F¹⁴. The helicity of the peptides 6F and 4F did not change appreciablyin the presence of lipid. However, these peptides were still lesshelical than peptides 2F and 5F. The CD results suggest that there is nosystematic change in the helicities of the peptides with increasingsubstitution by Phe; peptides 2F and 5F exhibited maximum helicity insolution and in the presence of phospholipid. TABLE 8 Helicities of theF-peptides in aqueous and lipid environments Percent Helicity PeptidesPBS¹ DMPC¹ 2F 55 72 3F³ 22 42 3F¹⁴ 19 55 4F 45 44 5F 55 76 6F 67 50 7F58 68

[0250] DSC Studies with DMPC and DiPoPE.

[0251] The effect of these 18A analogues on the chain melting transitionof multilamellar vesicles of DMPC was studied by DSC using peptide-lipidmixtures at 100:1 lipid/peptide molar ratio. Table 9 shows thetransition temperatures and enthalpies of the chain melting transitionof DMPC in the presence and absence of peptides. The pure lipidundergoes a pretransition at 13° C. and a main chain melting transitionat 23° C. The addition of the peptides to DMPC resulted in a broadeningof the gel to liquid-crystalline transition and a lowering of thetransition enthalpy (Table 9). The pretransition was not seen in thepresence of any of the peptides. Among the peptides studied, 2F, 3F³,5F, and 6F reduced the transition enthalpy to the maximum extent (Table9). None of the peptides changed the transition temperature by more than0.2° C. TABLE 9 Effect of the F-peptides on the chain melting transitionparameters of DMPC ΔH_(CM) Peptide T_(CM)(° C.) (kcals/mol) Δ_(T1/2) (°C.) DMPC 23.1 6.4 0.2 2F 23.2 4.5 0.5 3F³ 23.2 4.9 0.4 3F¹⁴ 23.2 5.5 0.34F 23.2 5.3 0.4 5F 23.2 4.9 0.5 6F 23.1 4.0 0.5 7F 23.2 4.5 0.5

[0252] The shift in the bilayer to hexagonal phase transitiontemperature (T_(H)) has been used to evaluate the effects of peptides onthe intrinsic curvature properties of phospholipids (Epand (1998)Biochim. Biophys. Acta, 1376: 353-368). It was previously shown that 2Fraises T_(H) of DiPoPE (Tytler et al. (1993) J. Biol. Chem. 268:22112-22118). In the current study we prepared the peptide-lipidmixtures in two ways. One was by adding the peptide in organic solventto the lipid in organic solvent followed by depositing the material as afilm and subsequently hydrating with buffer. In the other method, thepeptide and lipid were mixed after each was hydrated separately. If themixture comes to equilibrium prior to the DSC analysis, it should notmatter how the peptide and lipid are originally mixed. However, membranesystems can equilibrate slowly, in which case there may be more peptidein the lipid when it was incorporated at high concentrations into thelipid film. In general the results from both methods of samplepreparation are similar (not shown) but the shift in T_(H) tends to belarger for samples in which peptide was incorporated into a filmcomposed of lipid and peptide. The variation of the T_(H) with molfraction of peptide is shown for the various peptides and apo A-I (FIG.15). A linear increase in T_(H) is observed for 2F and 5F while 4Fbehaves more like apo A-I in that a more rapid increase is observed atlower peptide concentrations. On the other hand, the two 3F analogues aswell as 6F and 7F do not significantly affect T_(H).

[0253] Interaction of Peptides with Phospholipid Monolayers.

[0254] The monolayer exclusion pressure, π_(c), is the surface pressureat which peptides are no longer able to penetrate a monolayer of EPC.The value of π_(c), reflects the theoretical lipid affinity of thepeptide. The exclusion pressure of the F peptides increased withincreasing number of Phe residues (Table 7). All the peptides studiedhere had higher exclusion pressures than apo A-I and the parent peptide18A. The value of π_(c), increased gradually from 2F to 4F (38 to 40dyn/cm). This is in the range seen for 37pA, a tandem repeat of 18Apunctuated by a proline. The exclusion pressure value increasessignificantly for 5F, 6F and 7F (40 to 45 dyn/cm). It is apparent thatthe 5F, 6F and 7F homologues possess a similar ability to interact withEPC monolayers, as determined by the exclusion pressure. It isinteresting that the HPLC retention times and monolayer exclusionpressures for the F-peptides listed in Table 7 show parallel trends,with an abrupt increase between 4F and 5F.

[0255] Right Angle Light Scattering.

[0256] As can be seen in FIG. 16, all the peptides were able to clarifyEPC MLVs, unlike apo A-I, which does not clarify EPC MLVs. The twohomologous 3F peptides were the least effective in clarifying the EPCMLVs. The homologous peptides 2F, 5F, 6F and 7F, all clarified the EPCMLVs to a similar extents. Peptide 4F was the most effective inclarifying EPC MLVs with activity similar to that of Triton X-100. Thetime for 50% clearance of the turbidity of EPC MLVs was also theshortest for the homologue 4F. Peptide 7F took the longest time toachieve 50% clearance; this was due to an initial lag period of ˜300secs (FIG. 16). This is probably due to the requirement forself-associated 7F molecules to dissociate before they can interact withEPC MLVs and solubilize them. The slower rates of clearance exhibited bythe homologues 2F, 5F and 6F may also be due to a higherself-association of these peptides.

[0257] Activation of the Plasma Enzyme LCAT.

[0258] The ability of these peptides to activate the plasma enzyme LCATwas determined by measuring the initial velocity of the LCAT reactionwith egg PC-cholesterol vesicles as substrate (FIG. 17). LCAT activationis expressed relative to that by apo A-I, which was considered to be100%. Activation of LCAT by 20 μg/ml of peptides and apo A-I is shown inFIG. 4. At this concentration, apo A-I activates LCAT better than any ofthe peptides. Among the peptides studied here, however, 5F is the bestactivator (80% of apo A-I). As far as LCAT activation is concerned, both3F³ and 3F¹⁴ have similar activating abilities. Therefore, they havebeen represented as one bar (FIG. 17).

[0259] LDL-Induced Monocyte Chemotactic Activity

[0260] When LDL is incubated with the human artery wall coculturesystem, it is trapped in the subendothelial space and gets oxidized toproduce biologically active lipids. These lipids induce monocytechemotaxis. Thus, coculture monocyte chemotaxis is a well-establishedassay for the formation of biologically active lipids. It has been shownthat inhibition of chemotaxis is directly correlated with the removal of“seeding molecules” that are responsible for the secretion of monocytechemotactic protein-1 (MCP-1) (Navab et al. (2000) J. Lipid Res. 41:1481-1494; Navab et al. (2000) J. Lipid Res. 41: 1495-1508) anddifferentiation factor macrophage colony-stimulating factor (M-CSF).FIG. 18 shows that LDL after incubation with peptides exhibited variedeffects with homologues 4F, 5F and 6F reducing the chemotacticproperties of LDL the most. Peptides 3F were not at all effectivecompared to 2F and 7F, which were less effective than the peptides 4F,5F and 6F.

[0261] Discussion.

[0262] Effect of Increasing Hydrophobicity of a Class A AmphipathicHelical Peptide Analogue on its Physical-Chemical and Lipid BindingProperties:

[0263] The peptides studied in this paper are homologues of the parentpeptide, 18A . The calculated hydrophobicity per residue (according tomodified GES scale (Palgunachari et al. (1996) Arterioscler. Thromb.Vasc. Biol. 16: 328-338)) on the nonpolar face increased as the numberof Phe residues increased. This increase in hydrophobicity (Table 6) isreflected in the theoretical lipid affinity, Λ (Ibid.). However, the Λvalue increases gradually from 2F to 4F (from 13.03 to 14.59) with asudden increase in the value from 14.59 (for 4F) to 19.07 for 5F. Agradual increase in Λ was again observed after 5F in the values for 6Fand 7F (Table 6). This is due to the substitution of Leu at positions 3and 14 in Ac-18A-NH₂ with Phe which results in a slight increase in thehydrophobicity of the nonpolar face and thus, a slight increase in Λvalues for the two 3F analogues and 4F. In homologues 5F, 6F and 7Fhowever, besides the Leu to Phe substitutions, Ala in positions 11 and17 are also substituted by Phe, resulting in a significant increase inthe Λ values (Table 6). Since Ala is less hydrophobic than Leu and Leuis less hydrophobic than Phe, the substitution of Ala to Phe causes agreater change in hydrophobicity and theoretical lipid affinity of theresulting peptide than a Leu to Phe substitution.

[0264] The retention time on a C₁₈ reversed phase HPLC column,solubility of these peptides and their ability to penetrate an EPCmonolayer, all exhibit a trend similar to that seen in the theoreticallipid affinity values (Table 7). The retention times of peptides 2F,3F³, 3F ¹⁴ and 4F are about the same (21-22 min. ) and significantlyless than those of 5F, 6F and 7F, which comprise a second group (26-27min). The peptides 2F to 4F have considerably higher aqueous solubilitythan homologues 5F to 7F, which are sparingly soluble (Table 7). Agradual increase in exclusion pressure was observed from 2F to 4F afterwhich there is an abrupt increase from 40 dyn/cm to 45 dyn/cm. Theexclusion pressures for the peptides 5F, 6F and 7F are not verydifferent from each other and are significantly higher than that of apoA-I (Table 7). The parent peptide 18A (30 dyn/cm) and even the dimer of18A, 37pA (40 dyn/cm) were also significantly less effective inpenetrating into an egg PC monolayer spread at the air-water interface.Based on the above physical properties, the F peptides can be separatedinto two groups; group I with 2F, 3F³, 3F¹⁴, 4F and group II withpeptides 5F, 6F and 7F.

[0265] The CD data (Table 8) indicate that the percent helicity value ofall the peptides increases in the presence of DMPC suggesting that allof the peptides associate with lipids. The binding of these peptides toDMPC appears to be similar as suggested by DSC (Table 9). However, theeffect of these peptides on the stabilization of the bilayer structureof DiPoPE is different. 4F and 5F seem to interact better with DiPoPEbecause they appear to be better stabilizers than the other peptides.

[0266] While apo A-I is not able to clarify EPC MLVs, all of the peptideanalogs are able to do so, but to different extents. Among the group Ipeptides that are easily soluble in aqueous buffer and exhibit amonolayer exclusion pressure value in the range 38-40 dyn/cm (2F, 3Fanalogs and 4F), 4F appears to be the most efficient and at thepeptide:lipid ratio under investigation, exhibits similar kinetics tothat of Triton X-100 (FIG. 16). While the monolayer exclusion pressuresof the peptides 2F and 3F are similar, the 3F homologues are the slowestin clarifying EPC MLVs. The reason for reduced EPC clarifying ability ofthe 3F homologues is not clear at this time. The group II peptides (5F,6F and 7F) that are not easily soluble in aqueous buffer and possesssurface pressure values 45 dyn/cm solubilize EPC MLVs relatively slowly.These results are consistent with peptide aggregates having todisassociate and then interact with EPC. The superior reactivity of 4Fcan be explained by the fact that its hydrophobicity is optimal so thathydrophobic peptide:peptide interactions favoring self-association donot prevent peptide:lipid interactions.

[0267] Effect of Increased Hydrophobicity on LCAT Activation:

[0268] Activation of LCAT is a complex process and is not only dependenton lipid affinity but also on the interaction of the amphipathic helicalprotein with the enzyme LCAT (Jonas (2000) Biochim. Biophys. Acta 1529:245-256). In agreement with this, the ability to activate LCAT was foundto be different for the homologous peptides. The peptide 5F showed themaximum LCAT-activating ability, in agreement with the physicalproperties studied in Table 7 wherein an abrupt increase was seen from4F to 5F, including exclusion pressure values at the egg PC-waterinterface. The fact that the peptides 6F and 7F are not as effective as5F could be explained by the increased peptide:peptide interaction (asreflected in the low aqueous solubility of these peptides) which doesnot allow for peptide:lipid or peptide:LCAT interaction. These resultsare in agreement with our earlier observations with the 18A dimerpeptides in which the enhanced self-association of the dimer 18A-18A(36A) peptide reduced its ability to interact with lipids compared to18A-Pro-18A peptide (Jonas (2000) Biochiin. Biophys. Acta 1529:245-256). Although LCAT activation by the peptides has been comparedwith that of apo A-I, it should be noted that apo A-I and the peptidesinteract differently with the substrate since they all have differentreactivities to EPC (FIG. 16). Similar observations were made by Chunget al who showed that a synthetic peptide 18A-Pro-18A and apo A-Iinteract differently with EPC (Chung et al. (1985) J. Biol. Chem. 260:10256-10262).

[0269] Effect of Increased Hydrophobicity of the Nonpolar Face onLDL-Induced Monocyte Chemotaxis:

[0270] Since removal of “seeding molecules” depends on theamphipathicity of the peptide as reported by us (Navab et al. (2000) J.Lipid Res. 41: 1481-1494; Navab et al. (2000) J. Lipid Res. 41:1495-1508), we examined the ability of these peptides to inhibitLDL-induced monocyte chemotaxis. In this assay, peptides 4F, 5F and 6Fat 100 μg/ml level, showed significant and similar inhibition ofLDL-induced chemotaxis based on one way analysis of variance. Althoughthe homologue 2F showed some inhibitory activity, for reasons that arenot clear, peptide analogs 3F showed no inhibition compared to LDLalone. These results were in agreement with the fact that the peptide 3Fwas not able to remove the lipid hydroperoxides (results not shown) andthe reduced ability to clarify EPC MLVs. Peptide 7F was significantlyless effective than peptides 4F, 5F and 6F (P<0.001). The reducedability of 7F can again be explained by increased sell-association ofthe peptide that decreased its ability to interact with the lipid asseen in EPC MLV clarification studies. These results again demonstratethat the delicate balance existing between the contributions of thehydrophobicity of the peptide to self association can critically affectapo A-I mimicking properties.

[0271] In vivo administration of peptide 5F, which possesses increasedLCAT-activating ability and increased ability to remove “seedingmolecules” protected mice from diet-induced atherosclerosis. Incontrast, administration of 2F, that is similar in LCAT-activatingability to 4F, but less effective than 4F and 5F in removing “seedingmolecules” from LDL, did not significantly inhibit diet-induced lesionformation in C57 BL6 mice (mean lesion area for control miceadministered with PBS 14.7±1.8 μm²×10⁻³ compared to 2F-administered mice13.2±1.7 μm²×10⁻³, n=15). It follows that in this mouse model,inhibition of LDL-induced monocyte chemotaxis is more anti-atherogenicthan LCAT activation. Since the peptides 2F and 4F are similar inactivating LCAT, and 4F and 5F are similar in removing “seedingmolecules” from LDL, the peptide 4F may serve as a reagent todistinguish between the importance of LCAT activation and the inhibitionof LDL-induced monocyte chemotaxis in differentatherosclerosis-sensitive mouse models. If the inhibition of LDL-inducedchemotaxis is more important than the LCAT-activating ability, then 4Fshould be better peptide to use as an inhibitor of atherosclerosis sincethis peptide is more soluble than the peptides 5F, 6F and 7F.

Example 4 Peptides D-4F Maintains Paroxynase Levels and Blocks OxidizedPhospholipid Production During an Acute Inflammatory Response

[0272] We have observed that intranasal instillation of the influenza Avirus in mice caused a time dependent loss in the anti-inflammatoryproperties of HDL reaches a maximum 7 to 9 days after inoculation. Thedose chosen was one that did not cause viremia and so the changes werenot due directly to the virus but were due to the inflammatory stateinduced by the host's systemic response to the viral infection. Thisresponse is part of the innate immune system and is known as the acutephase reaction or acute phase response.

[0273] One of the consequences was dimunition in paraoxonase andplatelet activating acetylhydrolase activity in the HDL of the miceafter the influenza infection. As a result of the loss of these HDLenzymatic activites and also as a result of the association ofpro-oxidant proteins with HDL during the acute phase response, HDL wasno longer able to prevent LDL, oxidation and was no longer able toprevent the LDL-induced production of monocyte chemotactic activity byendothelial cells. Normal HDL is able to prevent the LDL-inducedproduction of monocyte chemotactic activity by endothelial cells becausenormal HDL contains sufficient paraoxonase and platelet activatingacetylhydrolase activities to destroy the biologically active oxidizedphospholipids.

[0274] In this example, we demonstrate that early (two days) afterinfluenza A infection the livers of infected mice generated theseoxidized phospholipids (FIG. 19) and later (7 to 9 days after infection)these biologically active oxidized phospholipids appeared in the aortaof the mice. However, if the mice were injected with 20 micrograms ofD-4F daily after infection with the influenza A virus paraoxonase levelsdid not fall (FIG. 20) and the biologically active oxidizedphospholipids were not generated beyond background (FIG. 21).

[0275] These data indicate that D-4F (and/or other peptides of thisinvention) can be given either orally or by injection to patients withknown coronary artery disease during influenza infection or other eventsthat can generate an acute phase inflammatory response (e.g. due toviral infection, bacterial infection, trauma, transplant, variousautoimmune conditions, etc.) and thus we can prevent by this short termtreatment the increased incidence of heart attack and stroke associatedwith pathologies that generate such inflammatory states.

Example 5 Oral Administration of an Apo A-I Mimetic Peptide Synthesizedfrom D-Amino Acids Dramatically Reduces Atherosclerosis in Mice

[0276] Apo A-I mimetic peptides synthesized from either D or L aminoacids were effective in vitro in protecting low density lipoprotein(LDL) against oxidation by artery wall cells. However, when the peptideswere given orally to LDL receptor null mice and their HLD was isolatedand tested for its ability to protect LDL against oxidation in vitro,only the peptides synthesized from D-amino acids were effective. Thepeptide synthesized from D-amino acids was stable in the circulation andwas found in fractions associated with high density lipoproteins (HDL).The peptide synthesized from L amino acids was rapidly degraded andexcreted in the urine. When the peptide synthesized from D-amino acidsknown as D-4F was administered orally, twice daily, to LDL receptor nullmice on a Western diet, lesions decreased by 79%. When added to thedrinking water of apo E null mice, D-4F decreased lesions by more than84%. We conclude that orally administered apo A-I mimetic peptidessynthesized from D-amino acids are useful for the prevention andtreatment of atherosclerosis and other chronic inflammatory illnessesthat are caused by oxidized lipids.

[0277] Background.

[0278] HDL-cholesterol concentrations are inversely related to the riskfor atherosclerotic coronary artery disease (Miller and Miller (1975)Lancet, 1: 16-19). Infusion (Badimon et al. (1990) J Clin Invest, 85:1234-1241) or transgenic expression (Plump et al. (1994) Proc. Natl.Acad. Sci., USA, 91: 9607-9611) of apo A-I, the major apolipoprotein ofHDL, has been shown to protect against atherosclerosis in animal models.The mechanisms by which apo A-I protects against the development ofatherosclerosis have been postulated to include reverse cholesteroltransport (Shah et al. (2001) Circulation, 103: 3047-3050) and removalof low levels of oxidized lipids, “seeding molecules”, that are requiredto oxidize LDL (Navab et al. (2000) J Lipid Res. 41: 1481-1494; Navab etal. (2000) J Lipid Res, 41: 1495-1508; Navab et al. (2001) ArterioslcerThromb Vasc Biol. 21: 481-488). Class A amphipathic helical peptideanalogs have been shown to mimic several in vitro properties of apoA-Iincluding the removal of the “seeding molecules” that are required forLDL oxidation (Navab et al. (2000) J Lipid Res. 41: 1481-1494; Navab etal. (2000) J Lipid Res, 41: 1495-1508). Intraperitoneal administrationof a class A amphipathic helical peptide has recently been shown toprotect mice from diet induced atherosclerosis without changing theirplasma cholesterol levels (Garber et al. (2001) J Lipid Res, 42:545-552). The reduction in lesions was associated with a significantimprovement in the ability of HDL to inhibit LDL oxidation in vitro(Id.). Up to now, the major limitation for the use of apo A-I or apo A-Imimetic peptides as a pharmacological agent has been the need for aparenteral route of administration.

[0279] Mammalian enzymes such as proteases recognize peptides andproteins synthesized from L-amino acids but rarely recognize thosesynthesized from D-amino acids. We demonstrate here that specificformulations of apo A-I mimetic peptides synthesized from D-amino acidscan be administered orally and dramatically inhibit atherosclerosis inmice.

[0280] Methods

[0281] Mice.

[0282] Female LDL receptor null or apo E null mice on a C57BL/6Jbackground were purchased from the Jackson Laboratory, Bar Harbor, Me.The LDL receptor null mice were maintained on Purina chow diet (RalstonPurina Co.) until they were 4 weeks old when they were switched to aWestern diet (Teklad, Madison, Wis., diet # 88137) for 6 weeks. The apoE null mice were maintained on Purina chow diet throughout the study LDLreceptor null mice received the test peptide or a vehicle control bygastric gavage twice daily for the periods indicated in the figurelegends. At four weeks of age the test peptide was added to the drinkingwater of some of the apo E null mice at the concentrations indicated inthe figure legends and the apo E null mice were continued on the chowdiet.

[0283] Mice were bled under anesthesia from the retro-orbital venousplexus, in accordance with protocols approved by the UCLA AnimalResearch Committee. Atherosclerotic lesions were measured as previouslydescribed⁹.

[0284] Lipoproteins

[0285] LDL and HDL were isolated as described (Navab et al. (2000) JLipid Res. 41: 1481-1494, and examples herein) after obtaining informedconsent from human volunteers and from the blood of mice as noted above.

[0286] Cocultures, Cellular Oxidation of LDL, Monocyte Isolation, andMonocyte Chemotaxis Assay

[0287] Human aortic endothelial and smooth muscle cells were isolatedand cultured as previously described (Id.). Cellular oxidation of LDL inthe presence and absence of HDL was determined as described (Id.). Humanblood monocytes were isolated after obtaining informed consent andmonocyte chemotaxis was determined as described previously⁵.

[0288] Synthesis and Preparation of apo A-I Mimetic Peptides

[0289] Apo A-I mimetic peptides were synthesized as previouslydescribed¹¹ except that in some instances each amino acid in the peptidewas the D-stereoisomer of the amino acid. The peptides are based on thesequence Ac-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-NH₂ (SEQ ID NO: 1)(Ac-18A-NH₂ or 2F). 2F or an analog of 2F with the primary amino acidsequence Ac-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH₂ (SEQ ID NO: 5, alsodesignated 4F) was used in the studies reported here. Peptidessynthesized from the L-amino acids are designated with an L (e.g. L-4F)and peptides synthesized from D-amino acids are designated with a D(e.g. D-4F). In some cases the peptides were iodinated using IODO-BEADreagent (Pierce, Rockford, Ill.) according to the recommendations by themanufacturer. Liposomes made ofL-α-1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (Avanti PolarLipids, Alabaster, Ala.) with and without D-4F were made according tothe manufacturer's recommendations. Extraction and detection of intactpeptides from mouse plasma was performed as described by Garber et al.(1992) Arterioscler Thromb. 12: 886-894, using reverse phase HPLC.

[0290] Other Methods

[0291] Protein (Navab et al. (2000) J Lipid Res. 41: 1481-1494) andcholesterol (Van Lenten et al. (2001) Circulation, 103: 2283-2288)content of lipoproteins and statistical analyses were performed asdescribed by Van Lenten et al. (2001) Circulation, 103: 2283-2288, withsignificance defined as P<0.05.

[0292] Results

[0293] In vitro, both L-2F and D-2F were equally able to block LDLoxidation and LDL-induced monocyte chemotactic activity in human arterywall cell cocultures (data not shown). It, vivo however, as shown inFIG. 22A after oral administration, only D-4F remained intact in thecirculation and was able to enhance HDL protective capacity (FIG. 22B)and decrease LDL-induced monocyte chemotactic activity (FIG. 22C). Twohours after oral administration of ¹²⁵I-L-4F or, 125I -D-4F the urinefrom mice given L-4F had approximately 15 times more radioactivity thanwas the case for mice given D-4F (data not shown).

[0294]FIG. 23, demonstrates that twice-daily administration of D-4F bygavage reduced atherosclerotic lesions in LDL receptor null mice on aWestern diet by 79%. Total plasma cholesterol levels did notsignificantly differ in the LDL receptor null mice given D-4F and thosegiven liposomes alone or saline alone. Total cholesterol was 761±69mg/dl for the D-4F group, 677±52 mg/dl for the liposome group and 699±31for the saline group. The mean HDL-cholesterol level was slightly higherin the D-4F group, 73±8.7 mg/dl, compared to 65.9±9.2 for the liposomegroup and 67.1±6.3 for the saline group, but these differences did notreach statistical significance.

[0295]FIG. 24 demonstrates that the apo E null mice given D-4F in theirdrinking water had more than an 84% reduction in lesions and was notsignificantly different whether the mice were given 2.5 mg/day/mouse or5.0 mg/day/mouse. There was no significant difference in the amount ofwater consumed (2.5 ml/day/mouse) between the apo E null mice receivingno peptide, or those receiving 2.5 mg D-4F/mouse/day or 5.0 mgD-4F/mouse/day and there was no significant difference in body, heart,or liver weights of the apo E null mice in the three groups (data notshown). Furthermore, the plasma total cholesterol concentration was notsignificantly different in the apo E null mice that received D-4F(478±149 mg/dl for mice receiving water without peptide, 534±12.3 mg/dlfor mice receiving D-4F at 2.5 mg/mouse/day, and 579±4.6 mg/dl for micereceiving D-4F at 5.0 mg/mouse/day). Mean HDL-cholesterol was mildlyincreased in the mice receiving D-4F but the differences (lid not reachstatistical significance (32.2±7 mg/dl for mice receiving water withoutpeptide, 38.7±5 for mice receiving D-4F at 2.5 mg/mouse/day, and 43.4±6mg/dl for mice receiving D-4F at 5.0 mg/mouse/day).

[0296] Discussion

[0297] Up to this point in time, the use of apo A-I and apo A-I mimeticpeptides as pharmacological agents has been limited by the need forparenteral administration. The marked reduction in atheroscleroticlesions in the present study occurred despite the absence of significantchanges in total plasma cholesterol. Although, there was a trend towardslightly higher HDL-cholesterol levels in the mice receiving D-4F, thisdid not reach statistical significance. The studies presented heresuggest that orally administered apo A-I mimetic peptides synthesizedfrom D-amino acids may be useful for the prevention and treatment ofatherosclerosis and other chronic inflammatory illnesses that are causedby oxidized lipids.

[0298] It is understood that the examples and embodiments describedherein are for illustrative purposes only and that various modificationsor changes in light thereof will be suggested to persons skilled in theart and are to be included within the spirit and purview of thisapplication and scope of the appended claims. All publications, patents,and patent applications cited herein are hereby incorporated byreference in their entirety for all purposes.

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Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 16 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 16 Glu Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 17 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 17 Glu Trp Leu Lys Ala PheTyr Asp Lys Val Phe Glu Lys Phe Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 18 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 18 Glu Trp Leu Lys Ala PheTyr Asp Lys Val Phe Glu Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 19 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 19 Glu Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 20 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 20 Glu Trp Leu Lys Ala PheTyr Asp Lys Val Phe Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 21 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 21 Ala Phe Tyr Asp Lys ValAla Glu Lys Leu Lys Glu Ala Phe 1 5 10 <210> SEQ ID NO 22 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 22 Ala Phe Tyr Asp Lys Val Ala Glu Lys Phe Lys Glu Ala Phe 1 510 <210> SEQ ID NO 23 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 23 Ala Phe Tyr Asp Lys ValAla Glu Lys Phe Lys Glu Ala Phe 1 5 10 <210> SEQ ID NO 24 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 24 Ala Phe Tyr Asp Lys Phe Phe Glu Lys Phe Lys Glu Phe Phe 1 510 <210> SEQ ID NO 25 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 25 Ala Phe Tyr Asp Lys PhePhe Glu Lys Phe Lys Glu Phe Phe 1 5 10 <210> SEQ ID NO 26 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 26 Ala Phe Tyr Asp Lys Val Ala Glu Lys Phe Lys Glu Ala Phe 1 510 <210> SEQ ID NO 27 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 27 Ala Phe Tyr Asp Lys ValAla Glu Lys Leu Lys Glu Phe Phe 1 5 10 <210> SEQ ID NO 28 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 28 Ala Phe Tyr Asp Lys Val Phe Glu Lys Phe Lys Glu Ala Phe 1 510 <210> SEQ ID NO 29 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 29 Ala Phe Tyr Asp Lys ValPhe Glu Lys Leu Lys Glu Phe Phe 1 5 10 <210> SEQ ID NO 30 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 30 Ala Phe Tyr Asp Lys Val Ala Glu Lys Phe Lys Glu Phe Phe 1 510 <210> SEQ ID NO 31 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 31 Lys Ala Phe Tyr Asp LysVal Phe Glu Lys Phe Lys Glu Phe 1 5 10 <210> SEQ ID NO 32 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 32 Leu Phe Tyr Glu Lys Val Leu Glu Lys Phe Lys Glu Ala Phe 1 510 <210> SEQ ID NO 33 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 33 Ala Phe Tyr Asp Lys ValAla Glu Lys Phe Lys Glu Ala Phe 1 5 10 <210> SEQ ID NO 34 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 34 Ala Phe Tyr Asp Lys Val Ala Glu Lys Leu Lys Glu Phe Phe 1 510 <210> SEQ ID NO 35 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 35 Ala Phe Tyr Asp Lys ValPhe Glu Lys Phe Lys Glu Ala Phe 1 5 10 <210> SEQ ID NO 36 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 36 Ala Phe Tyr Asp Lys Val Phe Glu Lys Leu Lys Glu Phe Phe 1 510 <210> SEQ ID NO 37 <211> LENGTH: 14 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 37 Ala Phe Tyr Asp Lys ValAla Glu Lys Phe Lys Glu Phe Phe 1 5 10 <210> SEQ ID NO 38 <211> LENGTH:14 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:<223> OTHER INFORMATION: synthetic or recombinant class A peptide <400>SEQUENCE: 38 Ala Phe Tyr Asp Lys Val Phe Glu Lys Phe Lys Glu Phe Phe 1 510 <210> SEQ ID NO 39 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 39 Asp Trp Leu Lys Ala LeuTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Leu <210> SEQ IDNO 40 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 40 Asp Trp Phe Lys Ala PheTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 41 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 41 Asp Trp Phe Lys Ala PheTyr Glu Lys Phe Phe Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 42 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 42 Glu Trp Leu Lys Ala LeuTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Leu <210> SEQ IDNO 43 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 43 Glu Trp Leu Lys Ala PheTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 44 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 44 Glu Trp Phe Lys Ala PheTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 45 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 45 Glu Trp Leu Lys Ala PheTyr Glu Lys Val Phe Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 46 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 46 Glu Trp Leu Lys Ala PheTyr Glu Lys Phe Phe Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 47 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 47 Glu Trp Phe Lys Ala PheTyr Glu Lys Phe Phe Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 48 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 48 Asp Phe Leu Lys Ala TrpTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Trp <210> SEQ IDNO 49 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 49 Glu Phe Leu Lys Ala TrpTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Trp <210> SEQ IDNO 50 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 50 Asp Phe Trp Lys Ala TrpTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Trp Trp <210> SEQ IDNO 51 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 51 Glu Phe Trp Lys Ala TrpTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Trp Trp <210> SEQ IDNO 52 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 52 Asp Lys Leu Lys Ala PheTyr Asp Lys Val Phe Glu Trp Ala Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 53 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 53 Asp Lys Trp Lys Ala ValTyr Asp Lys Phe Ala Glu Ala Phe Lys Glu 1 5 10 15 Phe Leu <210> SEQ IDNO 54 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 54 Glu Lys Leu Lys Ala PheTyr Glu Lys Val Phe Glu Trp Ala Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 55 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 55 Glu Lys Trp Lys Ala ValTyr Glu Lys Phe Ala Glu Ala Phe Lys Glu 1 5 10 15 Phe Leu <210> SEQ IDNO 56 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 56 Asp Trp Leu Lys Ala PheVal Asp Lys Phe Ala Glu Lys Phe Lys Glu 1 5 10 15 Ala Tyr <210> SEQ IDNO 57 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 57 Glu Lys Trp Lys Ala ValTyr Glu Lys Phe Ala Glu Ala Phe Lys Glu 1 5 10 15 Phe Leu <210> SEQ IDNO 58 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 58 Asp Trp Leu Lys Ala PheVal Tyr Asp Lys Val Phe Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 59 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 59 Glu Trp Leu Lys Ala PheVal Tyr Glu Lys Val Phe Lys Leu Lys Glu 1 5 10 15 Phe Phe <210> SEQ IDNO 60 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 60 Asp Trp Leu Arg Ala PheTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 61 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 61 Glu Trp Leu Arg Ala PheTyr Glu Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 62 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 62 Asp Trp Leu Lys Ala PheTyr Asp Arg Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 63 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 63 Glu Trp Leu Lys Ala PheTyr Glu Arg Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 64 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 64 Asp Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 65 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 65 Glu Trp Leu Lys Ala PheTyr Glu Lys Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 66 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 66 Asp Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Lys Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 67 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 67 Glu Trp Leu Lys Ala PheTyr Glu Lys Val Ala Glu Lys Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 68 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 68 Asp Trp Leu Lys Ala PheTyr Asp Arg Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 69 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 69 Glu Trp Leu Lys Ala PheTyr Glu Arg Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 70 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 70 Asp Trp Leu Arg Ala PheTyr Asp Lys Val Ala Glu Lys Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 71 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 71 Glu Trp Leu Arg Ala PheTyr Glu Lys Val Ala Glu Lys Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 72 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 72 Asp Trp Leu Arg Ala PheTyr Asp Arg Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 73 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 73 Glu Trp Leu Arg Ala PheTyr Glu Arg Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 74 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 74 Asp Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Arg Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 75 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 75 Glu Trp Leu Lys Ala PheTyr Glu Lys Val Ala Glu Arg Leu Arg Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 76 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 76 Asp Trp Leu Arg Ala PheTyr Asp Lys Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 77 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 77 Glu Trp Leu Arg Ala PheTyr Glu Lys Val Ala Glu Arg Leu Lys Glu 1 5 10 15 Ala Phe <210> SEQ IDNO 78 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 78 Asp Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe Pro Asp TrpLeu Lys Ala Phe Tyr Asp Lys Val Ala Glu Lys 20 25 30 Leu Lys Glu Ala Phe35 <210> SEQ ID NO 79 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 79 Asp Trp Leu Lys Ala PheTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Phe Phe Pro Asp TrpLeu Lys Ala Phe Tyr Asp Lys Val Ala Glu Lys 20 25 30 Leu Lys Glu Phe Phe35 <210> SEQ ID NO 80 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 80 Asp Trp Phe Lys Ala PheTyr Asp Lys Val Ala Glu Lys Leu Lys Glu 1 5 10 15 Ala Phe Pro Asp TrpPhe Lys Ala Phe Tyr Asp Lys Val Ala Glu Lys 20 25 30 Leu Lys Glu Ala Phe35 <210> SEQ ID NO 81 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 81 Asp Lys Leu Lys Ala PheTyr Asp Lys Val Phe Glu Trp Ala Lys Glu 1 5 10 15 Ala Phe Pro Asp LysLeu Lys Ala Phe Tyr Asp Lys Val Phe Glu Trp 20 25 30 Leu Lys Glu Ala Phe35 <210> SEQ ID NO 82 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 82 Asp Lys Trp Lys Ala ValTyr Asp Lys Phe Ala Glu Ala Phe Lys Glu 1 5 10 15 Phe Leu Pro Asp LysTrp Lys Ala Val Tyr Asp Lys Phe Ala Glu Ala 20 25 30 Phe Lys Glu Phe Leu35 <210> SEQ ID NO 83 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 83 Asp Trp Phe Lys Ala PheTyr Asp Lys Val Ala Glu Lys Phe Lys Glu 1 5 10 15 Ala Phe Pro Asp TrpPhe Lys Ala Phe Tyr Asp Lys Val Ala Glu Lys 20 25 30 Phe Lys Glu Ala Phe35 <210> SEQ ID NO 84 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 84 Asp Trp Leu Lys Ala PheVal Tyr Asp Lys Val Phe Lys Leu Lys Glu 1 5 10 15 Phe Phe Pro Asp TrpLeu Lys Ala Phe Val Tyr Asp Lys Val Phe Lys 20 25 30 Leu Lys Glu Phe Phe35 <210> SEQ ID NO 85 <211> LENGTH: 37 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <400> SEQUENCE: 85 Asp Trp Leu Lys Ala PheTyr Asp Lys Phe Ala Glu Lys Phe Lys Glu 1 5 10 15 Phe Phe Pro Asp TrpLeu Lys Ala Phe Tyr Asp Lys Phe Ala Glu Lys 20 25 30 Phe Lys Glu Phe Phe35 <210> SEQ ID NO 86 <211> LENGTH: 18 <212> TYPE: PRT <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetic orrecombinant class A peptide <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: (1)..(1) <223> OTHER INFORMATION: Xaa is aspartic acidor glutamic acid, or homologues or analogues thereo <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (2)..(3) <223> OTHER INFORMATION:Xaa is tryptophan, phenylalanine, alanine, leucine, tyrosine, isoleucine, valine, a-naphthylalanine, or homologues or analogues thereo<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (4)..(4)<223> OTHER INFORMATION: Xaa is lysine or arginine <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (5)..(5) <223> OTHER INFORMATION:Xaa is serine, threonine, alanine, glycine, histidine, or homologues oranalogues thereof <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (6)..(7) <223> OTHER INFORMATION: Xaa is tryptophan,phenylalanine, alanine, leucine, tyrosine, iso leucine, valine,a-naphthylalanine, or homologues or analogues thereo <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: (8)..(8) <223> OTHERINFORMATION: Xaa is aspartic acid or glutamic acid, or homologues oranalogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (9)..(9) <223> OTHER INFORMATION: Xaa is lysine or arginine<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (10)..(11)<223> OTHER INFORMATION: Xaa is tryptophan, phenylalanine, alanine,leucine, tyrosine, iso leucine, valine, a-naphthylalanine, or homologuesor analogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (12)..(12) <223> OTHER INFORMATION: Xaa is aspartic acid orglutamic acid, or homologues or analogues thereo <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (13)..(13) <223> OTHERINFORMATION: Xaa is serine, threonine, alanine, glycine, histidine, orhomologues or analogues thereof <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: (14)..(14) <223> OTHER INFORMATION: Xaa istryptophan, phenylalanine, alanine, leucine, tyrosine, iso leucine,valine, a-naphthylalanine, or homologues or analogues thereo <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (15)..(15) <223>OTHER INFORMATION: Xaa is serine, threonine, alanine, glycine,histidine, or homologues or analogues thereof <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (16)..(16) <223> OTHERINFORMATION: Xaa is aspartic acid or glutamic acid, or homologues oranalogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (17)..(18) <223> OTHER INFORMATION: Xaa is tryptophan,phenylalanine, alanine, leucine, tyrosine, iso leucine, valine,a-naphthylalanine, or homologues or analogues thereo <400> SEQUENCE: 86Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 1015 Xaa Xaa <210> SEQ ID NO 87 <211> LENGTH: 18 <212> TYPE: PRT <213>ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION:synthetic or recombinant class A peptide <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: (1)..(1) <223> OTHER INFORMATION: Xaa isaspartic acid or glutamic acid, or homologues or analogues thereo <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (8)..(8) <223>OTHER INFORMATION: Xaa is aspartic acid or glutamic acid, or homologuesor analogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (12)..(12) <223> OTHER INFORMATION: Xaa is aspartic acid orglutamic acid, or homologues or analogues thereo <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (16)..(16) <223> OTHERINFORMATION: Xaa is aspartic acid or glutamic acid, or homologues oranalogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (2)..(3) <223> OTHER INFORMATION: Xaa is tryptophan,phenylalanine, alanine, leucine, tyrosine, iso leucine, valine oralpha-naphthylalanine, or homologues or analogues thereo <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: (6)..(7) <223> OTHERINFORMATION: Xaa is tryptophan, phenylalanine, alanine, leucine,tyrosine, iso leucine, valine or alpha-naphthylalanine, or homologues oranalogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (10)..(11) <223> OTHER INFORMATION: Xaa is tryptophan,phenylalanine, alanine, leucine, tyrosine, iso leucine, valine oralpha-naphthylalanine, or homologues or analogues thereo <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: (14)..(14) <223> OTHERINFORMATION: Xaa is tryptophan, phenylalanine, alanine, leucine,tyrosine, iso leucine, valine or alpha-naphthylalanine, or homologues oranalogues thereo <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (17)..(18) <223> OTHER INFORMATION: Xaa is tryptophan,phenylalanine, alanine, leucine, tyrosine, iso leucine, valine oralpha-naphthylalanine, or homologues or analogues thereo <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: (4)..(4) <223> OTHERINFORMATION: Xaa is lysine or arginine <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: (9)..(9) <223> OTHER INFORMATION: Xaa islysine or arginine <220> FEATURE: <221> NAME/KEY: misc_feature <222>LOCATION: (13)..(13) <223> OTHER INFORMATION: Xaa is lysine or arginine<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (15)..(15)<223> OTHER INFORMATION: Xaa is lysine or arginine <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: (5)..(5) <223> OTHER INFORMATION:Xaa is serine, threonine, alanine, glycine, histidine, or homologues oranalogues thereo <400> SEQUENCE: 87 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa XaaXaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa

What is claimed is:
 1. A peptide that ameliorates a symptom ofatherosclerosis, wherein said peptide comprises an amino acid sequencethat: ranges in length from about 10 to about 30 amino acids; comprisesat least one class A amphipathic helix; comprises at least one “D” aminoacid residue; protects a phospholipid against oxidation by an oxidizingagent; and is not the D-18A peptide.
 2. The peptide of claim 1, whereinsaid peptide further comprises a protecting group.
 3. The peptide ofclaim 1, wherein said peptide further comprises a protecting groupcoupled to the amino or carboxyl terminus.
 4. The peptide of claim 2,wherein said protecting group is a protecting group selected from thegroup consisting of acetyl, amide, 3 to 20 carbon alkyl groups, Fmoc,t-boc, 9-fluoreneacetyl group, 1-fluorenecaboxylic group,9-florenecarboxylic group, 9-fluorenone-1-carboxylic group,beuzyloxycarbonyl, Xanthyl (Xan), Trityl (Trt), 4-methyltrityl (Mtt),4-methoxytrityl (Mmt), 4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr),Mesitylene-2-sulfonyl (Mts), 4,4=-dimethoxybenzhydryl (Mbh),Tosyl (Tos),2,2,5,7,8-pentamethyl chroman-6-sulphonyl (Pmc), 4-methylbenzyl (MeBzl),4-methoxybenzyl (MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl), Benzoyl (Bz),3-nitro-2-pyridinesulphenyl (Npys),1-(4,4-dimentyl-2,6-diaxocyclohexylidene)ethyl (Dde), 2,6-dichlorobenzyl(2,6-DiCl Bzl), 2-chlorobenzyloxycarbonyl (2-Cl-Z),2-bromobenzyloxycarbonyl (2-Br-Z), Benzyloxymethyl (Bom),t-butoxycarbonyl (Boc), cyclohexyloxy (cHxO), t-butoxymethyl (Bum),t-butoxy (tBuO), t-Butyl (tBu), Acetyl (Ac), a benzoyl group, acarbobenzoxy group, a propyl group, a butyl group, a pentyl group, ahexyl group group, and Trifluoroacetyl (TFA).
 5. The peptide of claim 1,wherein said peptide further comprises a first protecting group coupledto the amino terminus and a second protecting group coupled to thecarboxyl terminus.
 6. The peptide of claim 1, wherein all enantiomericamino acids are “D” amino acids.
 7. The peptide of claim 1, wherein saidpeptide has greater than about 50% amino acid sequence identity withhuman or mouse apo A-1.
 8. The peptide of claim 1, wherein said peptideis mixed with a pharmacologically acceptable excipient.
 9. The peptideof claim 1, wherein said peptide is mixed with a pharmacologicallyacceptable excipient suitable for oral administration to a mammal. 10.The peptide of claim 1, wherein said peptide comprises a sequenceselected from the group consisting ofD-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 2),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID N0:3),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 4),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 5),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 6),D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 7),D-W-F-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 8),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 9),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 10),D-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 11),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 12),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 13),E-W-L-K-L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 14),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 15),E-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 16),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 17),E-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 18),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 19),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 20),A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 21),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 22),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 23),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 24),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 25),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 26),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 27),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 28),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 29),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 30),K-A-F-Y-D-K-V-F-E-K-F-K-E-F- (SEQ ID NO: 31),L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 32),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 33),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 34),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 35),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 36),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 37),A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 38),D-W-L-K-A-L-Y-D-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 39),D-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 40),D-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 41),E-W-L-K-A-L-Y-E-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 42),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 43),E-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 44),E-W-L-K-A-F-Y-E-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 45),E-W-L-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 46),E-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 47),D-F-L-K-A-W-Y-D-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 48),E-F-L-K-A-W-Y-E-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 49),D-F-W-K-A-W-Y-D-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 50),E-F-W-K-A-W-Y-E-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 51),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 52),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 53),E-K-L-K-A-F-Y-E-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 54),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 55),D-W-L-K-A-F-V-D-K-F-A-E-K-F-K-E-A-Y- (SEQ ID NO: 56),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 57),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 58),E-W-L-K-A-F-V-Y-E-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 59),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 60),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 61),D-W-L-K-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 62), E-W-L-KA-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 63),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 64),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 65),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 66),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 67),D-W-L-K-A-F-Y-D-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 68),E-W-L-K-A-F-Y-E-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 69),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 70),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 71),D-W-L-R-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 72),E-W-L-R-A-F-Y-E-R-V-A-E-K L-K E-A-F- (SEQ ID NO: 73),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 74),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 75),D-W-L-R-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 76),E-W-L-R-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 77),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 78),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F(SEQ ID NO: 79),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 80),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-P-D-K-L-K-A-F-Y-D-K-V-F-E-W-L-K-E-A-F(SEQ ID NO: 81),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-P-D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L(SEQ ID NO: 82),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F(SEQ ID NO: 83),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-P-D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F(SEQ ID NO: 84),D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F(SEQ ID NO: 85).
 11. The peptide of claim 1, wherein said peptidecomprises the amino acid sequence of SEQ ID NO: 5 (F4).
 12. The peptideof claims 10 or 11, wherein all enantiomeric amino acids are “D” aminoacids.
 13. The peptide of claims 10 or 11, wherein said peptide furthercomprises a protecting group coupled to the amino or carboxyl terminus.14. The peptide of claim 13, wherein said peptide comprises a protectinggroup coupled to the amino terminal and said amino terminal protectinggroup is a protecting group selected from the group consisting of abenzoyl group, an acetyl, a propeonyl, a carbobenzoxy, a propyl, abutyl, a pentyl, a hexyl, and a 3 to 20 carbon alkyl.
 15. The peptide ofclaim 13, wherein said peptide comprises a protecting group coupled tothe carboxyl terminal and said carboxyl terminal protecting group is anamide.
 16. The peptide of claim 10, wherein said peptide furthercomprises a first protecting group coupled to the amino terminus and asecond protecting group coupled to the carboxyl terminus.
 17. Thepeptide of claim 16, wherein said peptide further comprises: a firstprotecting group coupled to (he amino terminus wherein said protectinggroup is a protecting group selected from the group consisting of abenzoyl group, an acetyl, a propeonyl, a carbobenzoxy, a propyl, abutyl, a pentyl, a hexyl, a hexyl, and a 3 to 20 carbon alkyl and asecond protecting group coupled to the carboxyl terminal and saidcarboxyl terminal protecting group is an amide.
 18. The peptide of claim16, wherein all enantiomeric amino acids are “D” amino acids.
 19. Thepeptide of claim 1, wherein said oxidizing agent is selected from thegroup consisting of hydrogen peroxide, 13(S)-HPODE, 15(S)-HPETE, HPODE,HPETE, HODE, and HETE.
 20. The peptide of claim 1, wherein saidphospholipid is selected from the group consisting of1-palmitoyl-2-arachidonoyl-sn-glycero 3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine (SAPE). 21.A composition suitable for oral administration that ameliorates asymptom of atherosclerosis, wherein said composition comprises a peptidethat is a human apo A-I peptide or an analogue of a human apo A-Ipeptide wherein said peptide has a first protecting group attached to anamino terminal and a second protecting group attached to a carboxylterminal and further wherein said peptide comprises a plurality of Damino acid residues.
 22. The composition of claim 21, wherein said firstprotecting group and said second protecting group are independentlyselected from the group consisting of an acetyl, amide, 3 to 20 carbonalkyl groups, Fmoc, t-boc, 9-fluoreneacetyl group, 1-fluorenecarboxylicgroup, 9-florenecarboxylic group, 9-fluorenone-1-carboxylic group,benzyloxycarbonyl, Xanthyl (Xan), Trityl (Trt), 4-methyltrityl (Mtt),4-methoxytrityl (Mmt), 4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr),Mesitylene-2-sulphonyl (Mts), 4,4═-dimethoxybenzhydryl (Mbh),Tosyl(Tos), 2,2,5,7,9-pentamethyl chroman-6-sulphonyl (Pmc), 4-methylbenzyl(MeBzl), 4-methoxybenzyl (MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl),Benzoyl (Bz), 3-nitro-2-pyridinesulphenyl (Npys),1-(4,4-dimentyl-2,6-diaxocyclohexylidene)ethyl (Dde), 2,6-dichlorobenzyl(2,6-DiCl-Bzl), 2-chlorobenzyloxycarbonyl (2-Cl-Z),2-bromobenzyloxycarbonyl (2 -Br-Z), Benzyloxymethyl (Bom),t-butoxycarbonyl (Boc), cyclohexyloxy(cHxO), t-butoxymethyl (Bum),t-butoxy (tBuO), t-Butyl (tBu), Acetyl (Ac), a benzoyl group, acarbobenzoxy group, a propyl group, a butyl group, a pentyl group, ahexyl group group, and Trifluoroacetyl (TFA).
 23. The composition ofclaim 21, wherein said first protecting group is an acetyl.
 24. Thecomposition of claim 21, wherein said second protecting group is anamide.
 25. The composition of claim 21, wherein more than half of theenantiomeric amino acids comprising said peptide are D amino acids. 26.The composition of claim 21, wherein all enantiomeric amino acidscomprising said peptide are D amino acids.
 27. The composition of claim21, wherein said composition further comprises a pharmaceuticallyacceptable excipient.
 28. The composition of claim 27, wherein saidexcipient is an excipient suitable for oral administration.
 29. Thecomposition of claim 27, wherein said excipient is an excipient suitablefor injection.
 30. The composition of claim 21, wherein said peptideprotects a phospholipid from oxidization by an oxidizing agent.
 31. Thecomposition of claim 30, wherein said oxidizing agent is selected fromthe group consisting of hydrogen peroxide, 13(S)-HPODE, 15(S)-HPETE,HPODE, HPETE, HODE, and HETE.
 32. The composition of claim 30, whereinsaid phospholipid is selected from the group consisting of1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero 3 phosphorylethanolamine (SAPE). 33.A method of ameliorating a symptom of atherosclerosis in a mammal, saidmethod comprising administering to said mammal a peptide or a concatamerof a peptide that: ranges in length from about 10 to about 30 aminoacids; comprises at least one class A amphipathic helix; comprises atleast one “D” amino acid residue; protects a phospholipid againstoxidation by an oxidizing agent; and is not the D-18A peptide.
 34. Themethod of claim 33, wherein said administering comprises orallyadministering said peptide.
 35. The method of claim 33, wherein saidmammal is a mammal diagnosed as having one or more symptoms ofatherosclerosis.
 36. The method of claim 33, wherein said organism is amammal diagnosed as at risk for atherosclerosis.
 37. The method of claim33, wherein said mammal is a human.
 38. The method of claim 33, whereinsaid mammal is non-human mammal.
 39. The method of claim 33, whereinsaid peptide further comprises a protecting group coupled to the aminoor carboxyl terminus.
 40. The method of claim 39, wherein saidprotecting group is a protecting group selected from the groupconsisting of acetyl, amide, 3 to 20 carbon alkyl groups, Fmoc, t-boc,9-fluoreneacetyl group, 1-fluorenecarboxylic group, 9-florenecarboxylicgroup, 9-fluorenone-1-carboxylic group, benzyloxycarbonyl, Xanthyl(Xan), Trityl (Trt), 4-methyltrityl (Mtt), 4-methoxytrityl (Mmt),4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr), Mesitylene-2-sulphonyl(Mts), 4,4═-(dimethoxybenzhydryl (Mbh),Tosyl (Tos),2,2,5,7,8-pentamethyl chroman-6-sulphonyl (Pmc), 4-methylbenzyl (MeBzl),4-methoxybenzyl (MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl), Benzoyl (Bz),3-nitro-2-pyridinesulphenyl (Npys),1-(4,4-dimentyl-2,6-diaxocyclohexylidene)ethyl (Dde), 2,6-dichlorobenzyl(2,6-DiCl-Bzl), 2-chlorobenzyloxycarbonyl (2-Cl-Z),2-bromobenzyloxycarbonyl (2-Br-Z), Benzyloxymethyl (Bom),t-butoxycarbonyl (Boc), cyclohexyloxy (cHxO), t-butoxymethyl (Bum),t-butoxy (tBuO), t-Butyl (tBu), Acetyl (Ac), a benzoyl group, acarbobenzoxy group, a propyl group, a butyl group, a pentyl group, ahexyl group group, and Trifluoroacetyl (TFA).
 41. The method of claim33, wherein said peptide further comprises a first protecting groupcoupled to the amino terminus and a second protecting group coupled tothe carboxyl terminus.
 42. The method of claim 33, wherein said peptidehas greater than about 50% amino acid sequence identity with human ormouse apo A-1.
 43. The method of claim 33, wherein said peptide is mixedwith a pharmacological excipient.
 44. The method of claim 33, whereinsaid peptide is mixed with a pharmacological excipient suitable for oraladministration to a mammal.
 45. The method of claim 33, wherein saidpeptide comprises an amino acid sequence selected from the groupconsisting of D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 2),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 3),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 4),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-(SEQ ID NO: 5),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 6),D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 7),D-W-F-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 8),D-W-L-K-A-F-Y-D-K-V-A-E-K L-K-E-F-F- (SEQ ID NO: 9),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 10),D-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 11),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 12),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 13),E-W-L-K-L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 14),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 15),E-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 16),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 17),E-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 18),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 19),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 20),A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 21),A-F-Y-D-K-V-A-E-K-F-K-E-A-F-(SEQ ID NO: 22),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 23),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 24),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 25),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 26),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 27),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 28),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 29),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 30),K-A-F-Y-D-K-V-F-E-K-F-K-E-F- (SEQ ID NO: 31),L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 32),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 33),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 34),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 35),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 36),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 37),A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 38),D-W-L-K-A-L-Y-D-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 39),D-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 40),D-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 41),E-W-L-K-A-L-Y-E-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 42),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 43),E-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 44),E-W-L-K-A-F-Y-E-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 45),E-W-L-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 46),E-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 47),D-F-L-K-A-W-Y-D-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 48),E-F-L-K-A-W-Y-E-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 49),D-F-W-K-A-W-Y-D-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 50),E-F-W-K-A-W-Y-E-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 51),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 52),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 53),E-K-L-K-A-F-Y-E-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 54),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 55),D-W-L-K-A-F-V-D-K-F-A-E-K-F-K-E-A-Y- (SEQ ID NO: 56),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 57),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 58),E-W-L-K-A-F-V-Y-E-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 59),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 60),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 61),D-W-L-K-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 62),E-W-L-K-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 63),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 64),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 65),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 66),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 67),D-W-L-K-A-F-Y-D-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 68),E-W-L-K-A-F-Y-E-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 69),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 70),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 71),D-W-L-R-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 72),E-W-L-R-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 73),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 74),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 75),D-W-L-R-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 76),E-W-L-R-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 77),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 78),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F(SEQ ID NO: 79),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 80),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-P-D-K-L-K-A-F-Y-D-K-V-F-E-W-L-K-E-A-F(SEQ ID NO: 81),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-P-D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L(SEQ ID NO: 82),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F(SEQ ID NO: 83),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-P-D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F(SEQ ID NO: 84),D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F(SEQ ID NO: 85).
 46. The method of claim 45, wherein all enantiomericamino acids comprising the peptide are D amino acids.
 47. The method ofclaim 45, wherein said peptide further comprises a protecting groupcoupled to the amino or carboxyl terminus.
 48. The method of claim 47,wherein said protecting group is a protecting group selected from thegroup consisting of acetyl, CH₃—(CH₂)_(n)—CO— where n ranges from 1 to20, and an amide.
 49. The method of claim 47, wherein said peptidefurther comprises a first protecting group coupled to the amino terminusand a second protectin group coupled to the carboxyl terminus.
 50. Themethod of claim 33, wherein said oxidizing agents is selected from thegroup consisting of hydrogen peroxide, 13(S)-HPODE, 15(S)-HPETE, HPODE,HPETE, HODE, and HETE.
 51. The method of claim 33, wherein saidphospholipid is selected from the group said phospholipid is selectedfrom the group consisting of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine (SAPE). 52.A method of ameliorating a symptom of atherosclerosis, said methodcomprising orally administering to an organism a composition thatcomprises a peptide that is a human apo A-I peptide or an analogue of ahuman apo A-I peptide wherein said peptide has a first protecting groupattached to an amino terminal and a second protecting group attached toa carboxyl terminal and further wherein said peptide comprises aplurality of D amino acid residues.
 53. The method of claim 74, whereinsaid organism is an organism diagnosed as having one or more symptoms ofatherosclerosis.
 54. The method of claim 74, wherein said organism is anorganism diagnosed as at risk for atherosclerosis.
 55. The method ofclaim 74, wherein said organism is a human.
 56. The method of claim 74,wherein said organism is non-human mammal.
 57. The method otf claim 74,wherein said first protecting group and said second protecting group areindependently selected from the group consisting of an acetyl, aCH₃—(CH₂)_(n)—CO— where n ranges from 3 to 20, and an amide.
 58. Themethod of claim 74, wherein said first protectin, group is an acetyl.59. The method of claim 74, wherein said second protecting group is anamide.
 60. The method of claim 74, wherein said composition furthercomprises a pharmaceutically acceptable excipient.
 61. The method ofclaim 60, wherein said excipient is ail excipient suitable for oraladministration.
 62. The method of claim 74, wherein said peptideprotects a phospholipid from oxidization by an oxidizing agent.
 63. Themethod of claim 62, wherein said oxidizing agent is selected from thegroup consisting of hydrogen peroxide, 13(S)-HPODE, 15(S)-HPETE, HPODE,HPETE, HODE, and HETE.
 64. The method of claim 62, wherein saidphospholipid is selected from the group consisting of1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC),1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAPC)),1-stearoyl-2-arachidonyl-sn-glycero-3-phosphorylethanolamine (SAPE). 65.A kit for ameliorating a symptom of atherosclerosis, said kit comprisinga container containing a peptide that: ranges in length from about 10 toabout 30 amino acids; comprises at least one class A amphipathic helix;comprises at least one D amino acid residue; protects a phospholipidagainst oxidation by an oxidizing agent; and is not the 18-A peptide.66. The kit of claim 65, wherein said peptide comprises a peptide havingan amino acid sequence selected from the group consisting ofD-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 2),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 3),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 4),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 5),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 6),D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 7),D-W-F-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 8),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 9),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 10),D-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E F-F- (SEQ ID NO: 11),D-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 12),D-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 13),E-W-L-K-L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 14),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 15),E-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 16),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 17),E-W-L-K-A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 18),E-W-L-K-A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 19),E-W-L-K-A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 20),A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 21),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 22),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 23),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 24),A-F-Y-D-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 25),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 26),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 27),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 28),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 29),A-F-Y-D-K-V-A-E-K-F-K-E-F-F- (SEQ ID NO: 30),K-A-F-Y-D-K-V-F-E-K-F-K-E-F- (SEQ ID NO: 31),L-F-Y-E-K-V-L-E-K-F-K-E-A-F- (SEQ ID NO: 32),A-F-Y-D-K-V-A-E-K-F-K-E-A-F- (SEQ ID NO: 33),A-F-Y-D-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 34),A-F-Y-D-K-V-F-E-K-F-K-E-A-F- (SEQ ID NO: 35),A-F-Y-D-K-V-F-E-K-L-K-E-F-F- (SEQ ID NO: 36), A-F-Y-D-K-V-A-E-K-F-K-E-FF- (SEQ ID NO: 37), A-F-Y-D-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 38),D-W-L-K-A-L-Y-D-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 39),D-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 40),D-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 41),E-W-L-K-A-L-Y-E-K-V-A-E-K-L-K-E-A-L- (SEQ ID NO: 42),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 43),E-W-F-K-A-F-Y-E-K-V-A-E-K-L-K-E-F-F- (SEQ ID NO: 44),E-W-L-K-A-F-Y-E-K-V-F-E-K-F-K-E-F-F- (SEQ ID NO: 45),E-W-L-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 46),E-W-F-K-A-F-Y-E-K-F-F-E-K-F-K-E-F-F- (SEQ ID NO: 47),D-F-L-K-A-W-Y-D-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 48),E-F-L-K-A-W-Y-E-K-V-A-E-K-L-K-E-A-W- (SEQ ID NO: 49),D-F-W-K-A-W-Y-D-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 50),E-F-W-K-A-W-Y-E-K-V-A-E-K-L-K-E-W-W- (SEQ ID NO: 51),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 52),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 53),E-K-L-K-A-F-Y-E-K-V-F-E-W-A-K-E-A-F- (SEQ ID NO: 54),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 55),D-W-L-K-A-F-V-D-K-F-A-E-K-F-K-E-A-Y- (SEQ ID NO: 56),E-K-W-K-A-V-Y-E-K-F-A-E-A-F-K-E-F-L- (SEQ ID NO: 57),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F- (SEQ ID NO: 58),E-W-L-K-A-F-V-Y-E-K-V-F-K L-K-E-F-F- (SEQ ID NO: 59),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 60),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 61),D-W-L-K-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 62),E-W-L-K-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 63),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 64),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 65),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 66),E-W-L-K-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 67),D-W-L-K-A-F-Y-D-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 68),E-W-L-K-A-F-Y-E-R-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 69),D-W-L-R-A-F-Y-D-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 70),E-W-L-R-A-F-Y-E-K-V-A-E-K-L-R-E-A-F- (SEQ ID NO: 71),D-W-L-R-A-F-Y-D-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 72),E-W-L-R-A-F-Y-E-R-V-A-E-K-L-K-E-A-F- (SEQ ID NO: 73),D-W-L-K-A-F-Y-D-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 74),E-W-L-K-A-F-Y-E-K-V-A-E-R-L-R-E-A-F- (SEQ ID NO: 75),D-W-L-R-A-F-Y-D-K-V-A-E R-L-K-E-A-F- (SEQ ID NO: 76),E-W-L-R-A-F-Y-E-K-V-A-E-R-L-K-E-A-F- (SEQ ID NO: 77),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 78),D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-V-A-E-K-L-K-E-F-F(SEQ ID NO: 79),D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-L-K-E-A-F(SEQ ID NO: 80),D-K-L-K-A-F-Y-D-K-V-F-E-W-A-K-E-A-F-P-D-K-L-K-A-F-Y-D-K-V-F-E-W-L-K-E-A-F(SEQ ID NO: 81),D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L-P-D-K-W-K-A-V-Y-D-K-F-A-E-A-F-K-E-F-L(SEQ ID NO: 82),D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-P-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F(SEQ ID NO: 83),D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F-P-D-W-L-K-A-F-V-Y-D-K-V-F-K-L-K-E-F-F(SEQ ID NO: 84),D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F-P-D-W-L-K-A-F-Y-D-K-F-A-E-K-F-K-E-F-F(SEQ ID NO: 85).
 67. The kit of claim 65, wherein all enantiomeric aminoacids comprising said peptide are D amino acids.
 68. The kit of claim65, wherein said peptide is combined with a pharmaceutically acceptableexcipient in a unit dosage formulation.
 69. The kit of claim 68, whereinsaid unit dosage formulation is for oral administration.
 70. The kit ofclaim 65, further comprising instructional materials teaching the use ofsaid peptide for ameliorating one or more symptoms of atherosclerosis.71. A kit for ameliorating a symptom of atherosclerosis, said kitcomprising a container containing a composition suitable for oraladministration that ameliorates a symptom of atherosclerosis, whereinsaid composition comprises a peptide that is a human apo A-I peptide oran analogue of a human apo A-I peptide, wherein said peptide has a firstprotecting group attached to an amino terminal and a second protectinggroup attached to a carboxyl terminal and further wherein said peptidecomprises a plurality of D amino acid residues.
 72. The kit of claim 71,wherein said peptide is combined with a pharmaceutically acceptableexcipient in a unit dosage formulation.
 73. The kit of claim 71, furthercomprising instructional materials teaching the use of said peptide forameliorating one or more symptoms of atherosclerosis.
 74. A method ofmitigating or preventing a coronary complication associated with anacute phase response to an inflammation in a mammal, wherein saidcoronary complication is a symptom of atherosclerosis, said methodcomprising administering to a mammal having said acute phase response,or at risk for said acute phase response, a polypeptide of any one ofclaims 1 through
 20. 75. The method of claim 74, where saidadministration is by a route selected from the group consisting of oraladministration, nasal administration, rectal administration,intraperitoneal injection, and intravascular injection, subcutaneousinjection, transcutaneous administration, and intramuscular injection.76. The method of claim 74, wherein said polypeptide is administered incombination with an all L-form of the same polypeptide.
 77. The methodof claim 74, wherein said polypeptide is provided as a unit formulationin a pharmaceutically acceptable excipient.
 78. The method of claim 74,wherein said acute phase response is an inflammatory response associatedwith a recurrent inflammatory disease.
 79. The method of claim 75,wherein said acute phase response is associated with a disease selectedfrom the group consisting of leprosy, tuberculosis, systemic lupuserythematosus, polymyalgia rheumatic, polyarteritis nodosa, scleroderma,idiopathic pulmonary fibrosis, chronic obstructive pulmonary disease,Alzheimers Disease and AIDS, polymyalgia rheumatica, polyalteritisnodosa, scleroderma, idiopathic pulmonary fibrosis, chronic obstructivepulmonary disease, Alzheimers Disease, AIDS, coronary calcification,calcific aortic stenosis, osteoporosis, and rheumatoid arthritis. 80.The method of claim 74, wherein said acute phase response is aninflammatory response associated with a condition selected from thegroup consisting of a bacterial infection, a viral infection, a fungalinfection, an organ transplant, a wound, an implanted prosthesis,parasitic infection, sepsis, endotoxic shock syndrome, and biofilmformation.
 81. A method of mitigating or preventing a coronarycomplication associated with an acute phase response to an inflammationin a mammal, wherein said coronary complication is a symptom ofatherosclerosis, said method comprising: assaying said mammal for anacute phase protein (APP) level indicative of an acute phase response ora significant risk of an acute phase response; and administering to amammal showing an acute phase protein (APP) level indicative of an acutephase response a polypeptide of any one of claims 1 through
 20. 82. Themethod of claim 81, wherein said acute phase protein (APP) is a postiveAPR selected from the group consisting of serum amyloid A, c-reactiveprotein, serum amyloid P component, C2 complement protein, C3 complementprotein, C4 complement protein, C5 complement protein, C9 complementprotein, B complement protein, C1 inhibitor, C4 binding protein,fibrinogen, von Willebrand factor, α1-antitrypsin, α1-antichymotrypsin,α2 antiplasmin, heparin cofactor II, plasminogen activator inhibitor I,haptoglobin, haemopexin, ceruloplasmin, manganese superoxide dismutase,α1-acid glycoprotein, haeme oxygenase, mannose binding protein,leukocyte protein I, lipoprotein (a), and lipopolysaccharide bindingprotein.
 83. The method of claim 81, wherein said acute phase protein(APP) is a negative APR selected from the group consisting of albumin,prealbumin, transferin, apoAI, apoAII, α2-HS glycoprotein,inter-α-trypsin inhibitor, histidine rich glycoprotein.